| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 86,762 | T→C | 10.0% | I378T (ATC→ACC) | ilvI → | acetolactate synthase III, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 86,762 | 0 | T | C | 10.0% | 159.3 / 9.0 | 60 | I378T (ATC→ACC) | ilvI | acetolactate synthase III, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (30/24); new base C (4/2); total (34/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
CAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAG > W3110S.gb/86695‑86826 | cAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCga < 1:905038/71‑1 (MQ=255) tGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGCCCGAGAc > 1:1885611/1‑71 (MQ=255) gcgcTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGActct < 1:3960877/70‑1 (MQ=255) cTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCg < 1:1544859/60‑1 (MQ=255) cTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCtt > 1:888559/1‑68 (MQ=255) tGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGAc < 1:692661/71‑1 (MQ=255) tGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGACCga < 1:333654/53‑1 (MQ=255) ccTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg > 1:3845591/1‑70 (MQ=255) ccTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACg > 1:3099897/1‑70 (MQ=255) ccTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGa < 1:2335644/71‑1 (MQ=255) tGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCga < 1:1893005/49‑1 (MQ=255) gAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAgg > 1:399546/1‑71 (MQ=255) gAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAgg > 1:2581203/1‑71 (MQ=255) aaaTATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGaa < 1:1033630/68‑1 (MQ=255) aaaTATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAgg > 1:4081490/1‑70 (MQ=255) aaTATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGga < 1:2429025/71‑1 (MQ=255) aaTATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGc > 1:686020/1‑57 (MQ=255) atatGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGga < 1:162135/70‑1 (MQ=255) tatGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCgaga > 1:2846089/1‑46 (MQ=255) tGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACg < 1:677392/71‑1 (MQ=255) tCACAGTGAAAAGATTAAACCGCAGGCGGTGATCga > 1:2089330/1‑36 (MQ=255) tCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACg > 1:3731500/1‑71 (MQ=255) ctcaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGAc < 1:1661309/61‑1 (MQ=255) cacaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAg > 1:2153262/1‑57 (MQ=255) cacaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGt > 1:558604/1‑71 (MQ=255) cacaGTGAAAAGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGt > 1:4279902/1‑71 (MQ=255) acaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCtt < 1:2667632/66‑1 (MQ=255) acaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGt > 1:1471331/1‑70 (MQ=255) acaGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGt < 1:249770/70‑1 (MQ=255) gAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTgg > 1:808339/1‑39 (MQ=255) gAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGa < 1:3626745/49‑1 (MQ=255) gAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTc > 1:4501945/1‑71 (MQ=255) aaaaGATTAAACCGCAGGCGGTGACCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCt < 1:2881226/59‑1 (MQ=255) aaaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGAc < 1:1944929/45‑1 (MQ=255) aaaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCg < 1:1327390/71‑1 (MQ=255) aaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGa > 1:3249315/1‑71 (MQ=255) aaGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGa > 1:3686821/1‑71 (MQ=255) aTTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGa > 1:2282115/1‑40 (MQ=255) aTTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCt < 1:3310711/54‑1 (MQ=255) aTTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTg > 1:405573/1‑60 (MQ=255) aTTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGt > 1:1124678/1‑71 (MQ=255) aTTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGt < 1:4191738/71‑1 (MQ=255) tAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGga > 1:1539705/1‑46 (MQ=255) tAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTc > 1:2221503/1‑70 (MQ=255) tAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCg > 1:1840703/1‑71 (MQ=255) aaaCCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGAc > 1:1454346/1‑48 (MQ=255) ccGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGa > 1:922646/1‑55 (MQ=255) ccGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCggg > 1:3545146/1‑69 (MQ=255) gCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGgcag > 1:1276664/1‑70 (MQ=255) cAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGgaga > 1:4611207/1‑41 (MQ=255) gCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGt < 1:358433/47‑1 (MQ=255) gCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCAcc < 1:1030961/70‑1 (MQ=255) cGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAg < 1:3096904/71‑1 (MQ=255) ggTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGa > 1:2993262/1‑47 (MQ=255) ggTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGa > 1:820178/1‑47 (MQ=255) gTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGc < 1:227987/38‑1 (MQ=255) gTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGc < 1:393582/38‑1 (MQ=255) gTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTAc > 1:4556857/1‑42 (MQ=255) gTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTAc > 1:3190441/1‑42 (MQ=255) gTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGt < 1:1316461/56‑1 (MQ=255) | CAGTGGCGCGCTCGTCAGTGCCTGAAATATGACACTCACAGTGAAAAGATTAAACCGCAGGCGGTGATCGAGACTCTTTGGCGGTTGACGAAGGGAGACGCTTACGTGACGTCCGATGTCGGGCAGCACCAG > W3110S.gb/86695‑86826 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |