| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,485,132 | G→A | 10.9% | S162N (AGC→AAC) | dsdA → | D‑serine ammonia‑lyase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,485,132 | 0 | G | A | 10.9% | 131.1 / 6.4 | 46 | S162N (AGC→AAC) | dsdA | D‑serine ammonia‑lyase |
| Reads supporting (aligned to +/- strand): ref base G (21/20); new base A (2/3); total (23/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.26e-01 | |||||||||||
TTGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCTTTAAG > W3110S.gb/2485062‑2485202 | ttGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg < 1:987611/71‑1 (MQ=255)ttGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg > 1:3151985/1‑71 (MQ=255)ttGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg > 1:2665428/1‑71 (MQ=255)ttGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg > 1:3952364/1‑71 (MQ=255)ttGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg > 1:913349/1‑71 (MQ=255)ttGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg > 1:1655860/1‑71 (MQ=255) gCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCa > 1:766106/1‑71 (MQ=255) aCGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGc < 1:2225095/71‑1 (MQ=255) gCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCt < 1:1801639/70‑1 (MQ=255) ttGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCtgtg > 1:3114615/1‑71 (MQ=255) tgatgatgaCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCa > 1:862725/1‑62 (MQ=255) atgatgaCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg < 1:2033114/58‑1 (MQ=255) atgatgaCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg < 1:401862/58‑1 (MQ=255) tgatgaCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAACATTGCTGTGGGCt > 1:4196449/1‑71 (MQ=255) atgaCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCAt < 1:379603/58‑1 (MQ=255) gaCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTg > 1:614353/1‑58 (MQ=255) aCTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAAc < 1:1920243/70‑1 (MQ=255) cAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTg > 1:1965635/1‑53 (MQ=255) cAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATc > 1:275045/1‑71 (MQ=255) aaCTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTg < 1:227018/71‑1 (MQ=255) aaCTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTg > 1:1469735/1‑71 (MQ=255) cTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAg > 1:1152068/1‑41 (MQ=255) cTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTggg > 1:3762314/1‑71 (MQ=255) gCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGt > 1:4177902/1‑71 (MQ=255) ttCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCa < 1:942827/38‑1 (MQ=255) ttCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCa < 1:3643112/38‑1 (MQ=255) ctcCGGAGTTTAAACAGTTCTTTAGCCAATACAGCAtt > 1:4433835/1‑38 (MQ=255) tcCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCaa > 1:3189336/1‑71 (MQ=255) ggAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATc > 1:2789368/1‑56 (MQ=255) ggAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCg > 1:2820167/1‑71 (MQ=255) gAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAAt > 1:3607524/1‑68 (MQ=255) tttAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAAcc < 1:3521514/45‑1 (MQ=255) tttAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAAcc < 1:4257098/45‑1 (MQ=255) tttAAACAGTTCTTTAGCCAATACAACATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCAt < 1:304573/71‑1 (MQ=255) tAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCATCCGGAAATCTGGGGTTATCAATCGGCAtt < 1:3533510/70‑1 (MQ=255) aaaCAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGTTTATCAATCGGCATTa < 1:2388262/70‑1 (MQ=255) aaCAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTAt > 1:100669/1‑70 (MQ=255) aaCAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATg < 1:3107404/71‑1 (MQ=255) gTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATc < 1:3112138/54‑1 (MQ=255) ttCTTTAGCCAATACAACATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAgc < 1:3345663/69‑1 (MQ=255) tCTTTAGCCAATACAACATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAgcgc < 1:3492752/70‑1 (MQ=255) cTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGccc > 1:3570480/1‑71 (MQ=255) cTTTAGCCAATACAACATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGa > 1:3491810/1‑65 (MQ=255) tAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCg < 1:3368862/69‑1 (MQ=255) tAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGc < 1:3283479/70‑1 (MQ=255) aGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCa < 1:2360754/70‑1 (MQ=255) aaTACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATc > 1:4368998/1‑43 (MQ=255) ttcAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTa < 1:1923213/50‑1 (MQ=255) aCAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCa < 1:2973821/41‑1 (MQ=255) aCAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCAtt > 1:2521557/1‑50 (MQ=255) cAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCttt > 1:3548403/1‑70 (MQ=255) cAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCttt > 1:2591785/1‑70 (MQ=255) cAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCttt < 1:1133098/70‑1 (MQ=255) cAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCTTTa < 1:3150159/71‑1 (MQ=255) aGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCaa > 1:671633/1‑40 (MQ=255) gCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCTTTAAg > 1:3808677/1‑71 (MQ=255) | TTGCTGACGCTTGATGATGACTACAGCAAACTGCTTTCTCCGGAGTTTAAACAGTTCTTTAGCCAATACAGCATTGCTGTGGGCTCAACCGGAAATCTGGGGTTATCAATCGGCATTATGAGCGCCCGCATTGGCTTTAAG > W3110S.gb/2485062‑2485202 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |