| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,508,088 | C→A | 23.5% | G615V (GGC→GTC) | ypdD ← | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,508,088 | 0 | C | A | 23.5% | 139.3 / 21.1 | 55 | G615V (GGC→GTC) | ypdD | fused predicted PTS enzymes Hpr component, enzyme I component, and enzyme IIA component |
| Reads supporting (aligned to +/- strand): ref base C (27/15); new base A (7/6); total (34/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCG > W3110S.gb/2508023‑2508158 | ggCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGt > 1:3500630/1‑71 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCg > 1:4498549/1‑70 (MQ=255) catactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGttacc > 1:3244432/1‑65 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:2012922/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCa > 1:2018249/1‑66 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCAcc > 1:2831667/1‑68 (MQ=255) atactcaGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGc < 1:417601/70‑1 (MQ=255) aGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGcc > 1:4137061/1‑71 (MQ=255) aGCTCATCAAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCaa > 1:781101/1‑66 (MQ=255) tCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCaaa > 1:4503226/1‑64 (MQ=255) cATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTAcc > 1:987755/1‑71 (MQ=255) cATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAg > 1:4213323/1‑52 (MQ=255) tCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCa < 1:4525653/59‑1 (MQ=255) ccAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCaaa > 1:937576/1‑60 (MQ=255) ccAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATg > 1:162272/1‑62 (MQ=255) ccAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTAc > 1:4505914/1‑67 (MQ=255) ccAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCAt > 1:1201818/1‑71 (MQ=255) cAGGCCCAGCCCAAGCAGTAGCGGCATATAACGGCTTTCACCGACCAGTTCACCGCa < 1:3995013/57‑1 (MQ=255) ccagcccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGcc > 1:3877366/1‑58 (MQ=255) ccagcccaAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCaaa > 1:323351/1‑54 (MQ=255) gcccaAGCAGTAGCGGCAGATAACGGCTTTCACCGcc < 1:1132791/37‑1 (MQ=255) cccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2555127/71‑1 (MQ=255) ccaAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTg < 1:3062116/71‑1 (MQ=255) caAGCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCaaa > 1:3728082/1‑48 (MQ=255) gCAGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2814217/66‑1 (MQ=255) aGTAGCGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATgcgc < 1:4523455/71‑1 (MQ=255) gTAGCGGCAGATAAGGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCAGCTGATGCGCa < 1:3178810/71‑1 (MQ=255) tAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAg > 1:841079/1‑71 (MQ=255) cGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTAccc < 1:2852069/47‑1 (MQ=255) cGGCAGATAACGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTgg < 1:89602/71‑1 (MQ=255) gCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGt > 1:3765581/1‑70 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa > 1:992983/1‑71 (MQ=255) aTAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTa > 1:1464028/1‑71 (MQ=255) tAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAt < 1:2861335/71‑1 (MQ=255) tAACGGCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAt > 1:2329399/1‑71 (MQ=255) aaCGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCAtt > 1:1519614/1‑42 (MQ=255) aaCGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATgcg > 1:598859/1‑57 (MQ=255) aCGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCa > 1:1766849/1‑58 (MQ=255) aCGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAtt < 1:3730444/70‑1 (MQ=255) aCGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAtt < 1:748707/70‑1 (MQ=255) aCGGCTTTCACCGACCAGTTCACCGCAAATGCCTTCCCATTTGCCCCGCt < 1:3313116/50‑1 (MQ=255) cGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtg < 1:2655117/71‑1 (MQ=255) ggCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCa > 1:3988327/1‑56 (MQ=255) ggCTTTCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGAt > 1:2321157/1‑51 (MQ=255) cTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGa > 1:3858910/1‑48 (MQ=255) cTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGa > 1:3853674/1‑48 (MQ=255) tttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCt < 1:2007578/45‑1 (MQ=255) ttCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTtgctgc > 1:623726/1‑70 (MQ=255) tCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAtt > 1:707730/1‑63 (MQ=255) tCACCGACCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACt > 1:4168716/1‑60 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACt > 1:53677/1‑59 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:3191622/70‑1 (MQ=255) cACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCaa < 1:3085012/70‑1 (MQ=255) aCCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTAt < 1:507000/60‑1 (MQ=255) aCCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAAc < 1:2813402/70‑1 (MQ=255) gCCCAGTTCACC‑CAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATgcg < 1:2173346/71‑1 (MQ=255) cccAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATgcg < 1:3666101/71‑1 (MQ=255) | GGCTACTCATACTCAGCTCATCCAGGCCCAGCCCAAGCAGTAGCGGCAGATAACGGCTTTCACCGCCCAGTTCACCGCAAATGCCTACCCATTTGCCCCGCTGATGCGCAGTGGTAACTATTTGCTGCAACATGCG > W3110S.gb/2508023‑2508158 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |