| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,516,776 | G→A | 13.8% | intergenic (+3/+138) | ypeC → / ← mntH | conserved hypothetical protein/manganese/divalent cation transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,516,776 | 0 | G | A | 13.8% | 155.1 / 14.5 | 58 | intergenic (+3/+138) | ypeC/mntH | conserved hypothetical protein/manganese/divalent cation transporter |
| Reads supporting (aligned to +/- strand): ref base G (22/28); new base A (6/2); total (28/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
ATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTT > W3110S.gb/2516706‑2516846 | aTCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAAcg > 1:588796/1‑71 (MQ=255) tCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAAcgc > 1:1069414/1‑71 (MQ=255) gTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGt > 1:1076381/1‑71 (MQ=255) gAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTcc > 1:1631023/1‑71 (MQ=255) gAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTcc > 1:4053825/1‑71 (MQ=255) aaGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACACGTCCg > 1:4443963/1‑71 (MQ=255) ggCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGc > 1:2467499/1‑70 (MQ=255) ggCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACACGTCCGcc < 1:4092260/71‑1 (MQ=255) tGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCTTCCGcc < 1:573813/68‑1 (MQ=255) cgcgATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAAcgc > 1:2823886/1‑58 (MQ=255) gATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGcc < 1:3277397/62‑1 (MQ=255) aTCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGcac < 1:4643228/71‑1 (MQ=255) aTCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGcac < 1:3243188/71‑1 (MQ=255) aTCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGcac < 1:4438703/71‑1 (MQ=255) tCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTc > 1:4220098/1‑56 (MQ=255) gTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAAt < 1:1112407/71‑1 (MQ=255) cGATCATCGCCGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCt < 1:3587348/71‑1 (MQ=255) gATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTg < 1:3363612/71‑1 (MQ=255) atcGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACACGTCCGCCAATGGAGCACAATGCCTGATGc > 1:2435361/1‑71 (MQ=255) tcGCGGAAAAGGCCTGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATg < 1:2972143/52‑1 (MQ=255) cgcgGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATg < 1:2371377/51‑1 (MQ=255) cgcgGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGa > 1:3660009/1‑71 (MQ=255) gcgGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGAc > 1:100106/1‑71 (MQ=255) ggAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGc < 1:3491309/71‑1 (MQ=255) gAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCt < 1:3081964/71‑1 (MQ=255) aaaaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGcac < 1:765699/52‑1 (MQ=255) aaaGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACaa > 1:3892487/1‑53 (MQ=255) aaaGGCCGGGGTCATGGGCACCGCCATTAAACACGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAg > 1:2744278/1‑71 (MQ=255) ccGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGcat < 1:867860/46‑2 (MQ=255) gggTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCt < 1:785522/70‑1 (MQ=255) gggTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCt < 1:4433411/70‑1 (MQ=255) tCATGGGCACCGCCATTAAACGCGTCCGCCAATgg < 1:868066/35‑1 (MQ=255) tCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCt < 1:4446665/47‑1 (MQ=255) cATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACaa > 1:3703473/1‑41 (MQ=255) aTGGGGACCGCCATTAAACGCGTCCGCCAATTGAGCACAATGCCTGATGCGACGCTAGTGCGTc < 1:4038207/64‑1 (MQ=255) aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCt < 1:3401015/56‑1 (MQ=255) aTGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAg > 1:3171881/1‑71 (MQ=255) aTGGGCACCGCCATTAAACACGTCCGCCAATGGAGCACaa > 1:3512848/1‑40 (MQ=255) tGGGCACCGCCATTAAACACGTCCGCCAATGGAGCACAATGCCTGATGCGACGCt < 1:1618370/55‑1 (MQ=255) gggCACCGCCATTAAACACGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGc > 1:746714/1‑71 (MQ=255) ggCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGa > 1:2591503/1‑49 (MQ=255) ggCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCt > 1:4572077/1‑62 (MQ=255) ggCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGcc < 1:3391778/71‑1 (MQ=255) cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCt < 1:3110334/48‑1 (MQ=255) cGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACaa < 1:878171/71‑1 (MQ=255) gCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGa > 1:2073494/1‑43 (MQ=255) ccATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTAt < 1:889468/58‑1 (MQ=255) ccATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAAc > 1:4406983/1‑71 (MQ=255) ccATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAAc > 1:3665393/1‑71 (MQ=255) aTTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCg < 1:2427792/71‑1 (MQ=255) ttAAACGCGTCCGCCAATGGAGCACAATGCCTGATGc < 1:3558583/37‑1 (MQ=255) ttAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGt < 1:1461624/50‑1 (MQ=255) aaaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGa > 1:1482026/1‑37 (MQ=255) aaaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCt > 1:2267177/1‑41 (MQ=255) aaaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGc > 1:943519/1‑58 (MQ=255) aaaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCgg > 1:1379404/1‑69 (MQ=255) aaaCGCGTCCGCAAATGGAGCACAATGCCTGATGCGa > 1:2086087/1‑37 (MQ=255) aaaCACGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCtt > 1:973841/1‑51 (MQ=255) aaCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTATTGCGTCTTATCAGGCCTACAAACCGGctc < 1:1008851/71‑1 (MQ=255) aCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTa > 1:2030879/1‑50 (MQ=255) aCGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTa > 1:1206258/1‑50 (MQ=255) gcgTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCttt < 1:3465027/71‑1 (MQ=255) gcgTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCttt < 1:1113481/71‑1 (MQ=255) gcgTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCtt > 1:2882728/1‑70 (MQ=255) | ATCGTGAAGGCTGGCGCGATCGTGACGATCATCGCGGAAAAGGCCGGGGTCATGGGCACCGCCATTAAACGCGTCCGCCAATGGAGCACAATGCCTGATGCGACGCTAGTGCGTCTTATCAGGCCTACAAACCGGCTCTTT > W3110S.gb/2516706‑2516846 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |