| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,611,108 | T→C | 13.0% | F185L (TTC→CTC) | hyfR → | DNA‑binding transcriptional activator, formate sensing |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,611,108 | 0 | T | C | 13.0% | 258.5 / 28.1 | 100 | F185L (TTC→CTC) | hyfR | DNA‑binding transcriptional activator, formate sensing |
| Reads supporting (aligned to +/- strand): ref base T (46/41); new base C (6/7); total (52/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.69e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.10e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
ATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTG > W3110S.gb/2611040‑2611175 | aTGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCAtttttt < 1:2384361/70‑1 (MQ=255)aTGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTc > 1:4486573/1‑71 (MQ=255)aTGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTc < 1:2785649/71‑1 (MQ=255) tGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCg < 1:171003/71‑1 (MQ=255) cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCgg > 1:532853/1‑70 (MQ=255) cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCgg < 1:2827039/70‑1 (MQ=255) cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGt > 1:3990527/1‑71 (MQ=255) cAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGt < 1:378757/71‑1 (MQ=255) gTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCt < 1:4376955/71‑1 (MQ=255) gTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGt < 1:48497/69‑1 (MQ=255) cTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTc > 1:1592840/1‑67 (MQ=255) cTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTgg > 1:2033971/1‑70 (MQ=255) cTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTgg < 1:3380963/70‑1 (MQ=255) gTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt > 1:1140568/1‑71 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:321594/70‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:1513970/70‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:3346596/70‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:3758642/70‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:1287853/70‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCtt < 1:4595205/70‑1 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCtt > 1:3068494/1‑70 (MQ=255) tCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCt < 1:1096813/69‑1 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCAttttt > 1:1806512/1‑56 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGt > 1:4458401/1‑61 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag > 1:1378662/1‑71 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag > 1:1302330/1‑71 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag < 1:1237081/71‑1 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag < 1:4477032/71‑1 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag < 1:4471677/71‑1 (MQ=255) acaTCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcag < 1:4036523/71‑1 (MQ=255) ttGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGt > 1:2516881/1‑56 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:3246725/68‑1 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:3684943/68‑1 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTcagtcagt < 1:3636893/69‑1 (MQ=255) gATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTa < 1:1470591/70‑1 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGt > 1:1585442/1‑53 (MQ=255) aTCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTAt < 1:2571540/70‑1 (MQ=255) tcGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc < 1:2710340/55‑1 (MQ=255) gacgaTCTGATCGCTGCCGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACt > 1:3112764/1‑71 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCAtttttt > 1:4274483/1‑44 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATgg > 1:2712712/1‑67 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt < 1:4048359/68‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt < 1:863050/68‑1 (MQ=255) gacgaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACt > 1:2726128/1‑71 (MQ=255) gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGc < 1:477593/50‑1 (MQ=255) gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca > 1:2206104/1‑58 (MQ=255) gaTCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca > 1:3026753/1‑58 (MQ=255) aTCTGATCGCTGACGTCGCTCGTGAGATCCATCAtttttt < 1:617997/40‑1 (MQ=255) aTCTGATCGCTGACGTCGCTCGTGAGATCCATCAtttttt < 1:3420027/40‑1 (MQ=255) tCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGc > 1:2515667/1‑70 (MQ=255) cTGATCGCTGACGTCTCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtca > 1:1883378/1‑51 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtca > 1:4375239/1‑51 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTg > 1:930972/1‑66 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTg > 1:1915734/1‑66 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa > 1:509681/1‑71 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa > 1:2631525/1‑71 (MQ=255) cTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGa > 1:2776700/1‑71 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTa > 1:1275774/1‑55 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatca > 1:1919418/1‑71 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat < 1:365643/69‑1 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat > 1:237182/1‑69 (MQ=255) aTCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtc < 1:2050631/47‑1 (MQ=255) tCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtcag < 1:1244292/52‑1 (MQ=255) tCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGt > 1:3480080/1‑58 (MQ=255) tCGCTGACGTCGCTCGTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGatcat > 1:226397/1‑71 (MQ=255) gACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtca > 1:599238/1‑42 (MQ=255) gACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtca > 1:12786/1‑42 (MQ=255) gACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc > 1:1212118/1‑55 (MQ=255) aCGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGa > 1:4258498/1‑68 (MQ=255) aCGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAg < 1:1761381/71‑1 (MQ=255) aCGTCGCTCGTGAGATCAATCATTTTCTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAg < 1:2042432/71‑1 (MQ=255) cGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGa < 1:3398848/71‑1 (MQ=255) gCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca > 1:4561932/1‑40 (MQ=255) gCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca > 1:21188/1‑40 (MQ=255) gCTCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca > 1:1314160/1‑40 (MQ=255) gCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa > 1:675441/1‑48 (MQ=255) gCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTAc > 1:930699/1‑49 (MQ=255) gCTCGTGAGATCCATCATTTTCTCGGTCTGGCTtca > 1:90265/1‑36 (MQ=255) cTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgag < 1:2251146/71‑1 (MQ=255) tCGTGAGATCCATCATTTTTTCGGTCTGGCTtcagtca < 1:3486561/38‑1 (MQ=255) gTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACgag < 1:3158210/68‑1 (MQ=255) gTGAGATCCATCATTTTCTCGGTCTGGCTTCAGTCAGTa > 1:4215709/1‑39 (MQ=255) gagaTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTa < 1:3416505/42‑1 (MQ=255) aTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGaa > 1:1159679/1‑59 (MQ=255) aTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGt < 1:652574/67‑1 (MQ=255) aTCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAg > 1:862079/1‑71 (MQ=255) ccATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGat < 1:689400/46‑1 (MQ=255) ccATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAAc > 1:4511917/1‑58 (MQ=255) acatcatTTTCTCGGTCTGGCTTCAGACAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCac > 1:3155111/2‑68 (MQ=255) catcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGcc < 1:208221/70‑1 (MQ=255) atcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtg < 1:4502441/71‑1 (MQ=255) atcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtg < 1:2457631/71‑1 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGa < 1:2009281/53‑1 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGa < 1:170702/53‑1 (MQ=255) tcatTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgt < 1:4197913/71‑1 (MQ=255) catTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgtg > 1:2172104/1‑71 (MQ=255) catTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCtgtg > 1:2912328/1‑71 (MQ=255) atTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtgg > 1:3037342/1‑71 (MQ=255) ttttCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtggtg > 1:3463742/1‑70 (MQ=255) ttttCCGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTgtggtg < 1:225623/70‑1 (MQ=255) | ATGCAGTGCTGTCACATCTTGATCTCGACGATCTGATCGCTGACGTCGCTCGTGAGATCCATCATTTTTTCGGTCTGGCTTCAGTCAGTATGGTACTGGGCGATCATCGAAAGAACGAGAAGTTCAGCCTGTGGTG > W3110S.gb/2611040‑2611175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |