| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,629,497 | C→T | 9.4% | N172N (AAC→AAT) | yfgI → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,629,497 | 0 | C | T | 9.4% | 225.1 / 9.8 | 74 | N172N (AAC→AAT) | yfgI | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (30/37); new base T (3/4); total (33/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACC > W3110S.gb/2629430‑2629567 | aCGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACaa < 1:2839267/70‑1 (MQ=255)aCGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACaa < 1:1009770/70‑1 (MQ=255) ccAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCg < 1:1478916/71‑1 (MQ=255) cAACAGAAAGATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCg > 1:1142142/1‑70 (MQ=255) aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGa > 1:2868689/1‑70 (MQ=255) aCAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGAc < 1:2452267/71‑1 (MQ=255) gAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGc < 1:3762434/71‑1 (MQ=255) atGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGtt > 1:637918/1‑71 (MQ=255) tGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAATAAATCGGACAGCTGGttt < 1:4247396/71‑1 (MQ=255) cTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTttaat > 1:16082/1‑71 (MQ=255) gAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTttaat > 1:4132680/1‑69 (MQ=255) gAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTttaat > 1:3829110/1‑69 (MQ=255) aGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTttaa > 1:164788/1‑66 (MQ=255) gCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCg < 1:2213191/71‑1 (MQ=255) gCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCg < 1:1294193/71‑1 (MQ=255) cGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAATAAATCGGa > 1:2941100/1‑51 (MQ=255) cGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGa > 1:204669/1‑71 (MQ=255) gTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAAc < 1:4397065/41‑1 (MQ=255) gTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACaa < 1:1326284/43‑1 (MQ=255) gTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACaa < 1:684826/43‑1 (MQ=255) gTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACaa < 1:4548219/43‑1 (MQ=255) tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACa < 1:1847132/41‑1 (MQ=255) tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGttt < 1:4497864/58‑1 (MQ=255) tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGtt < 1:1485747/57‑1 (MQ=255) tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGtt < 1:3181186/57‑1 (MQ=255) tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGt > 1:1535753/1‑71 (MQ=255) tCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGt > 1:1530308/1‑71 (MQ=255) tCGCGATCTGATGAAAAGCGTGGCCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGt < 1:941386/71‑1 (MQ=255) cgcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAAt > 1:1137912/1‑43 (MQ=255) gcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGttt < 1:3550124/56‑1 (MQ=255) gcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGt > 1:2352059/1‑69 (MQ=255) gcgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGt > 1:1837105/1‑69 (MQ=255) cgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAg < 1:4222523/71‑1 (MQ=255) cgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAg < 1:1974301/71‑1 (MQ=255) cgATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAg < 1:3142892/71‑1 (MQ=255) gATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTttaa > 1:2789211/1‑56 (MQ=255) aTCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACaa < 1:1569960/37‑1 (MQ=255) tCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTaa > 1:206230/1‑71 (MQ=255) cTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGa > 1:2999771/1‑41 (MQ=255) cTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTttaat > 1:4388360/1‑54 (MQ=255) tgatgaAAAGCGTGGGCAAAGCAGAAGAGAATAAATCGGACAGCTGGTTTAATTAATCGATGTTa > 1:1507665/1‑65 (MQ=255) gatgaAAAGCGTGGGCAAAGCAGAAGAGAATAAATCGGACAGCTGGTTTAATTAATc < 1:706843/57‑1 (MQ=255) atgaAAAGCGTTGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGt < 1:1522200/65‑1 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGa > 1:3534813/1‑38 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCt > 1:715114/1‑43 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGt < 1:1517979/65‑1 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTACCtt > 1:577729/1‑70 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACtt < 1:4012061/70‑1 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACtt > 1:550785/1‑70 (MQ=255) atgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACtt < 1:3302197/70‑1 (MQ=255) tgaAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATc < 1:3435095/55‑1 (MQ=255) aaaaGCGTGGGCAAAGCAGAAGAGAATAAATCGGACa < 1:269230/37‑1 (MQ=255) aaaaGCGTGGGCAAAGCAGAAGAGAATAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAAt > 1:476946/1‑71 (MQ=255) aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGa > 1:328409/1‑35 (MQ=255) aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAAc > 1:2321397/1‑65 (MQ=255) aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCaa < 1:4055153/70‑1 (MQ=255) aaaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCaa > 1:2040592/1‑70 (MQ=255) aaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATc < 1:4346635/71‑1 (MQ=255) aaaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATc < 1:3417471/71‑1 (MQ=255) aaGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATcc < 1:3777839/71‑1 (MQ=255) cGTGGGCAAAGCAGAAGAGAATAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCtat < 1:3339121/71‑1 (MQ=255) cGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATACtat < 1:4476067/71‑1 (MQ=255) aaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATc < 1:981849/40‑1 (MQ=255) aaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATc < 1:1499798/40‑1 (MQ=255) aaGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGt < 1:3296134/45‑1 (MQ=255) gCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTg > 1:1463795/1‑69 (MQ=255) agaagaGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTg > 1:3655352/1‑67 (MQ=255) gaagaGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAg > 1:2710856/1‑69 (MQ=255) aagaGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGa > 1:345449/1‑69 (MQ=255) agagAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATaa < 1:3535314/71‑1 (MQ=255) agagAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATaa < 1:924621/71‑1 (MQ=255) gagaACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATaa > 1:4041762/1‑70 (MQ=255) aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTa < 1:4335826/39‑1 (MQ=255) aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATaaaaaa < 1:2573745/71‑1 (MQ=255) aaCAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATaaaaaa > 1:754188/1‑71 (MQ=255) cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAAc < 1:4290982/39‑1 (MQ=255) cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCt > 1:4526106/1‑48 (MQ=255) cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAAcc < 1:2123048/71‑1 (MQ=255) cAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAAcc < 1:2058302/71‑1 (MQ=255) | ACGCCAACAGAAATATGACTGAAGGCGGTCGCGATCTGATGAAAAGCGTGGGCAAAGCAGAAGAGAACAAATCGGACAGCTGGTTTAATTAATCGATGTTAGTAACTTCAATCCTATAATTCTTGAAGATAAAAAACC > W3110S.gb/2629430‑2629567 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |