| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,629,792 | T→C | 27.6% | D467G (GAC→GGC) | guaA ← | GMP synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,629,792 | 0 | T | C | 27.6% | 115.4 / 56.3 | 76 | D467G (GAC→GGC) | guaA | GMP synthetase |
| Reads supporting (aligned to +/- strand): ref base T (27/28); new base C (8/13); total (35/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.78e-01 | |||||||||||
GTACGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGAC > W3110S.gb/2629722‑2629862 | gTACGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGt < 1:2738335/71‑1 (MQ=255)gTACGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGCTTTCGACAGCACCCAGAGAGACAACCCAGt > 1:4315257/1‑71 (MQ=255) cGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCa < 1:2867101/70‑1 (MQ=255) cGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCAt < 1:3527571/71‑1 (MQ=255) cGGCAGATGCGCCCAGTGTGCGGTCATAAAGCCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCAt < 1:2841793/71‑1 (MQ=255) aGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACtt < 1:1511436/71‑1 (MQ=255) aGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACt > 1:1135949/1‑70 (MQ=255) aTGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTAc < 1:4174217/71‑1 (MQ=255) tGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTAc > 1:2945542/1‑70 (MQ=255) tGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTAc > 1:138968/1‑70 (MQ=255) cgcCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGAc > 1:2164192/1‑71 (MQ=255) gcCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGAcc < 1:153947/71‑1 (MQ=255) ccAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCAt < 1:1269018/59‑1 (MQ=255) ccAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCAt < 1:385507/59‑1 (MQ=255) gtgtGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGc < 1:1883674/71‑1 (MQ=255) tgCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACt > 1:51094/1‑56 (MQ=255) cGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCAt < 1:3052416/70‑1 (MQ=255) cGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCAt > 1:1089939/1‑70 (MQ=255) cGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCAt < 1:1193715/70‑1 (MQ=255) gTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATAc > 1:2428000/1‑51 (MQ=255) gTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTAc > 1:1879649/1‑71 (MQ=255) tCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACt < 1:4187055/51‑1 (MQ=255) tCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCAt < 1:2590486/67‑1 (MQ=255) cATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTa > 1:1966915/1‑68 (MQ=255) cATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGc > 1:3115664/1‑71 (MQ=255) aTAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACt < 1:3473253/49‑1 (MQ=255) aTAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGc > 1:2469799/1‑70 (MQ=255) aaGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAAc > 1:4090520/1‑71 (MQ=255) aaGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAAc > 1:4132649/1‑71 (MQ=255) aaGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAAc > 1:4138044/1‑71 (MQ=255) gTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACgg < 1:3665922/71‑1 (MQ=255) tCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACgg > 1:574413/1‑70 (MQ=255) tCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGAcc < 1:3484535/50‑1 (MQ=255) tCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGAcc < 1:3807205/50‑1 (MQ=255) tCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCAt > 1:2621322/1‑52 (MQ=255) cGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTAc > 1:3851775/1‑45 (MQ=255) cGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTAc > 1:841780/1‑45 (MQ=255) cGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTAc > 1:3265287/1‑45 (MQ=255) tGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGt < 1:3102453/71‑1 (MQ=255) tGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGt < 1:97328/71‑1 (MQ=255) tGGTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGt < 1:916742/71‑1 (MQ=255) ggTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTa > 1:913363/1‑71 (MQ=255) ggTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTa > 1:2389551/1‑71 (MQ=255) gTTTCGACAGCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGt < 1:3179208/69‑1 (MQ=255) ttCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCAt > 1:2329795/1‑51 (MQ=255) ttCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGc > 1:4264128/1‑56 (MQ=255) aGCACGCAGAGAGACAACCCAGTCATACTTACGATCATCGCCCAt < 1:4334498/45‑1 (MQ=255) gCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGaa < 1:641004/71‑1 (MQ=255) gCACGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGaa < 1:2471501/71‑1 (MQ=255) aCGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca > 1:1562796/1‑71 (MQ=255) cGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAacac < 1:2581379/71‑1 (MQ=255) cGCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAacac < 1:2376701/71‑1 (MQ=255) gCAGAGAGACAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca > 1:55500/1‑69 (MQ=255) agagagACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACgg < 1:579704/49‑1 (MQ=255) agagagACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACgg < 1:1727893/49‑1 (MQ=255) gagagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGaa < 1:93684/64‑1 (MQ=255) gagagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTg < 1:3579251/71‑1 (MQ=255) gagagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTg < 1:242127/71‑1 (MQ=255) gagagaCAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACTGAACGTACCGGCAGGAacac < 1:38508/67‑1 (MQ=255) agagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGaa > 1:4525429/1‑49 (MQ=255) agagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGa < 1:1468373/71‑1 (MQ=255) gagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAc > 1:2792638/1‑53 (MQ=255) gagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAc > 1:2269893/1‑53 (MQ=255) gagaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca > 1:1340829/1‑64 (MQ=255) agaCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAAc < 1:3723011/71‑1 (MQ=255) aCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACTGAACGTACCGGCAGGAaca < 1:404214/61‑1 (MQ=255) aCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAacac < 1:3384239/62‑1 (MQ=255) aCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca < 1:449620/61‑1 (MQ=255) aCAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca < 1:901057/61‑1 (MQ=255) aCAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGaa > 1:3435116/1‑59 (MQ=255) aCAACCCAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAaca < 1:3308536/61‑1 (MQ=255) cAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACTcc < 1:857711/71‑1 (MQ=255) cAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAAcaca > 1:1556534/1‑57 (MQ=255) cAGCCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCt > 1:1538906/1‑71 (MQ=255) aGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTAc > 1:2495943/1‑43 (MQ=255) aGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCg < 1:3996180/45‑1 (MQ=255) gTCATACTTACGACCATCGCCCATTACGCCAACGGAAc > 1:480715/1‑38 (MQ=255) gTCATACTTACGACCATCGCCCATTACGCCAACGGAAc > 1:2218138/1‑38 (MQ=255) tCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGc > 1:340575/1‑45 (MQ=255) tCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGAc < 1:90140/71‑1 (MQ=255) tCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGAc < 1:1803434/71‑1 (MQ=255) | GTACGGCAGATGCGCCCAGTGTGCGGTCATAAAGTCGATGGTTTCGACAGCACGCAGAGAGACAACCCAGTCATACTTACGACCATCGCCCATTACGCCAACGGAACGTACCGGCAGGAACACAGTGAACGCCTGGCTGAC > W3110S.gb/2629722‑2629862 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |