| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,635,048 | T→C | 12.7% | K322R (AAG→AGG) | der ← | predicted GTP‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,635,048 | 0 | T | C | 12.7% | 137.6 / 12.5 | 55 | K322R (AAG→AGG) | der | predicted GTP‑binding protein |
| Reads supporting (aligned to +/- strand): ref base T (22/26); new base C (3/4); total (25/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.71e-01 | |||||||||||
AAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCC > W3110S.gb/2634982‑2635110 | aaGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTAt < 1:2289395/70‑1 (MQ=255) cAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTcacca > 1:759702/1‑71 (MQ=255) cAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTcacca > 1:465543/1‑71 (MQ=255) aCGGAAGTCCAGCGTTTCTTTCTCCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTcac < 1:2967077/66‑1 (MQ=255) ggAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTc > 1:3164296/1‑62 (MQ=255) ggAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAAt > 1:4590405/1‑70 (MQ=255) ggAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCACACTTATTcacca > 1:3103268/1‑66 (MQ=255) gTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTca > 1:1647615/1‑59 (MQ=255) tCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTAtt < 1:2692969/56‑1 (MQ=255) tCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACa < 1:3061988/69‑1 (MQ=255) cAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTcac < 1:4252692/57‑1 (MQ=255) cAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAg > 1:3633966/1‑69 (MQ=255) aGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTcac < 1:2339578/56‑1 (MQ=255) cGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAgc > 1:3566482/1‑71 (MQ=255) cGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACCTATTCACCACAATGACAAGTGAgc < 1:3585961/71‑1 (MQ=255) tctttcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGcc > 1:3356089/1‑70 (MQ=255) tcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACt > 1:1658046/1‑39 (MQ=255) tcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAg > 1:3924586/1‑58 (MQ=255) tcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCAc < 1:3949299/69‑1 (MQ=255) tcACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTa > 1:2160467/1‑71 (MQ=255) aCCTGCTCTTTCACTTCCTGACTTAGGCCATCCCACCTATTCACCACAATGACAAGTGAGCGCCCACTAt < 1:4217957/70‑1 (MQ=255) aCCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTc > 1:3665232/1‑42 (MQ=255) ccTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTg < 1:1572500/71‑1 (MQ=255) cTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACa < 1:4599610/52‑1 (MQ=255) ctctTTCACTTCCTGACTCAGGCCATCCCACTTAtt > 1:4133758/1‑36 (MQ=255) ctctTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGaa > 1:639925/1‑71 (MQ=255) ctctTTCACTTCCTGACTCAGGCCATCCCACCTATTCACCACaa > 1:1380122/1‑44 (MQ=255) ctctTTCACTTCCTGACTCAGGCCATCCCACCTATTCACCACAATGACAAGTGAGCGCCCACTATTgag < 1:4079043/69‑1 (MQ=255) tctTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCAc > 1:2571072/1‑61 (MQ=255) tctTTCACTTCCTGACTCAGGCCATCCCACCTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGaa > 1:4117581/1‑70 (MQ=255) tttCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAAt < 1:4303641/42‑1 (MQ=255) tttCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAAt < 1:282322/69‑1 (MQ=255) tCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAg > 1:3240276/1‑46 (MQ=255) tCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGt > 1:1712017/1‑47 (MQ=255) tCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATaa < 1:638556/69‑1 (MQ=255) cACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGc > 1:4141213/1‑71 (MQ=255) aCTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGACCGCCCACTATTGAGAATAAAGc < 1:2163872/70‑1 (MQ=255) cTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGAc < 1:1015679/40‑1 (MQ=255) cTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGAc < 1:79371/40‑1 (MQ=255) cTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGccc < 1:1652776/71‑1 (MQ=255) tCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAg < 1:4547325/41‑1 (MQ=255) tCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCcag > 1:3512894/1‑71 (MQ=255) ccTGACTCAGGCCATCCCACTTATTCACCACAATGACAAg > 1:941837/1‑40 (MQ=255) ccTGACTCAGGCCATCCCACTTATTCACCACAATGACAAg > 1:4508690/1‑40 (MQ=255) cTGACTCAGGCCATCCCACTTATTCACCACAATGACa < 1:158725/37‑1 (MQ=255) cTGACTCAGGCCATCCCACTTATTCACCACAATGACa < 1:1007645/37‑1 (MQ=255) cTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCga < 1:1315898/71‑1 (MQ=255) cAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCgaga < 1:3045325/71‑1 (MQ=255) aGGCCATCCCACCTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCgag < 1:2269928/69‑1 (MQ=255) ccATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTgaga < 1:3045011/48‑1 (MQ=255) ccATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTgaga < 1:587272/48‑1 (MQ=255) cATCCCACCTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATcc > 1:3207751/1‑71 (MQ=255) aTCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAAt > 1:2870837/1‑48 (MQ=255) ccACTTATTCACCACAATGACAAGTGAGCGCCCACt < 1:159358/36‑1 (MQ=255) cACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGaa < 1:4362080/43‑1 (MQ=255) aCTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCgaga < 1:1175275/60‑1 (MQ=255) ttATTCACCACAATGACAAGTGAGCGCCCACTATTGAGaa < 1:4291332/40‑1 (MQ=255) ttATTCACCACAATGACAAGTGAGCGCCCACTATAGAGAATAAAg > 1:2988420/1‑45 (MQ=255) | AAGCCCAGACGGAAGTCCAGCGTTTCTTTCACCTGCTCTTTCACTTCCTGACTCAGGCCATCCCACTTATTCACCACAATGACAAGTGAGCGCCCACTATTGAGAATAAAGCCCAGCAGCGAGAGATCC > W3110S.gb/2634982‑2635110 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |