| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,637,539 | A→G | 13.6% | V134A (GTT→GCT) | yfgM ← | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,637,539 | 0 | A | G | 13.6% | 107.8 / 10.1 | 44 | V134A (GTT→GCT) | yfgM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/18); new base G (3/3); total (23/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
CACCCTTCACCTTTGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGCCCCTGTTGTA > W3110S.gb/2637473‑2637608 | cACCCTTCACCTTTGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAAcgcg < 1:2380774/71‑1 (MQ=255) ccTTCACCTTTGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAGCGCGAGc < 1:511418/71‑1 (MQ=255) cACCTTTGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCaa < 1:3183910/69‑1 (MQ=255) cTTTGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACg > 1:3416495/1‑70 (MQ=255) tGATGTTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCa < 1:3169696/69‑1 (MQ=255) tGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAg < 1:788553/70‑1 (MQ=255) aTGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAtt < 1:4396575/71‑1 (MQ=255) tGGTATCAAGGGTTTTCAGCGCGGCATCAGCGTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAttt < 1:1215011/71‑1 (MQ=255) tGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAttt < 1:1452671/71‑1 (MQ=255) tGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAttt < 1:216254/71‑1 (MQ=255) gTATCAAGGGTTTTCAGCGCGTCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTAt < 1:4220830/71‑1 (MQ=255) tCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCaa < 1:1190301/55‑1 (MQ=255) cAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGa > 1:662162/1‑56 (MQ=255) cAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGa > 1:1854469/1‑56 (MQ=255) cAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGa > 1:4550408/1‑56 (MQ=255) aaGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAGCGCGAGCAAGACGCAGATTTATCACg > 1:1524662/1‑70 (MQ=255) aaGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACgg < 1:980298/71‑1 (MQ=255) gggTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCt > 1:4642408/1‑71 (MQ=255) ggCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAtt > 1:1181066/1‑48 (MQ=255) aTCAGCCTGCTTGAGCTGTACCTGTACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGAttt < 1:630182/64‑1 (MQ=255) aTCAGCCTGCTTGAGCTGTACCTGAACGCGAGCaa > 1:4645605/1‑35 (MQ=255) aTCAGCCTGCTAGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCg > 1:3481961/1‑70 (MQ=255) aGCCTGCTTGAGCTGTACCTGCACGCGAGCAAGACGCAGATTTATCa > 1:3783472/1‑47 (MQ=255) aGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAtt > 1:1147457/1‑42 (MQ=255) cTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCgtgt > 1:420127/1‑71 (MQ=255) tGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGAtt < 1:4064443/38‑1 (MQ=255) tGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCa > 1:1912843/1‑43 (MQ=255) tGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTc > 1:3964827/1‑71 (MQ=255) gCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTga < 1:1489544/51‑1 (MQ=255) gCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCt > 1:3103621/1‑71 (MQ=255) cTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTa > 1:726027/1‑38 (MQ=255) cTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTa > 1:2306140/1‑38 (MQ=255) cTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTg > 1:2842935/1‑71 (MQ=255) tGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCt > 1:2080684/1‑68 (MQ=255) gAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGc < 1:2246451/69‑1 (MQ=255) gTACCTGAGCGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGccc > 1:302814/1‑70 (MQ=255) gTACCTGAGCGCGAGCAAGACGCAGATTTATCACGGCTTTAAGATTTTCATCGCTCGTGTCTGCCAGccc > 1:1666310/1‑70 (MQ=255) gTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTga < 1:324709/42‑1 (MQ=255) tACCTGAGCGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGcccc < 1:188992/70‑1 (MQ=255) tACCTGAGCGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGcccc < 1:2499157/70‑1 (MQ=255) tACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCa > 1:87227/1‑49 (MQ=255) tACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGcccc < 1:469388/70‑1 (MQ=255) tACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGcccc < 1:483601/70‑1 (MQ=255) ccTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGCCCCtgt > 1:1107811/1‑71 (MQ=255) aaCGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGCCCCtgttgt < 1:735821/70‑1 (MQ=255) aCGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGCCCCTGTTGTa < 1:4530641/70‑1 (MQ=255) | CACCCTTCACCTTTGATGGTATCAAGGGTTTTCAGCGCGGCATCAGCCTGCTTGAGCTGTACCTGAACGCGAGCAAGACGCAGATTTATCACGGCTTTGAGATTTTCATCGCTCGTGTCTGCCAGCCCCTGTTGTA > W3110S.gb/2637473‑2637608 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |