| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 123,736 | A→G | 11.4% | K240K (AAA→AAG) | aceE → | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 123,736 | 0 | A | G | 11.4% | 184.1 / 13.8 | 70 | K240K (AAA→AAG) | aceE | pyruvate dehydrogenase, decarboxylase component E1, thiamin‑binding |
| Reads supporting (aligned to +/- strand): ref base A (25/37); new base G (3/5); total (28/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AAAGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTGTAACCTGCAG > W3110S.gb/123668‑123805 | aaaGATACCTCTAAACAAACCGTTTACGCTTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCaaa < 1:1013646/69‑1 (MQ=255)aaaGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAggg > 1:3109039/1‑71 (MQ=255)aaaGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAg > 1:244701/1‑70 (MQ=255) aaGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGt > 1:2778123/1‑71 (MQ=255) aaGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGt < 1:2081780/71‑1 (MQ=255) aGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAGGGTg > 1:1005488/1‑71 (MQ=255) aCCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGAt > 1:2052017/1‑71 (MQ=255) aCCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGAt > 1:4057726/1‑71 (MQ=255) ccTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATc < 1:2995223/71‑1 (MQ=255) tctAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATc < 1:470738/69‑1 (MQ=255) ctAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAGGGTGCGATCAc > 1:4328051/1‑70 (MQ=255) tAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCAc < 1:1546259/69‑1 (MQ=255) tAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCa > 1:1739516/1‑71 (MQ=255) aacaaacCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATc > 1:1086761/1‑71 (MQ=255) caaacCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAGGGTGCGATCACCATCGc < 1:1811893/71‑1 (MQ=255) aaacCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCt < 1:3153064/71‑1 (MQ=255) aaacCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCt < 1:3032568/71‑1 (MQ=255) aacCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCt < 1:1503845/70‑1 (MQ=255) acCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCAc > 1:1577792/1‑62 (MQ=255) acCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCAc > 1:2507260/1‑62 (MQ=255) tttACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGc < 1:522985/52‑1 (MQ=255) tttACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGc < 1:3387661/52‑1 (MQ=255) tttACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGc > 1:621040/1‑64 (MQ=255) tACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTa > 1:4592906/1‑64 (MQ=255) tACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTa > 1:4598140/1‑64 (MQ=255) tACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTg > 1:2786449/1‑70 (MQ=255) tACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTg > 1:3745838/1‑70 (MQ=255) tACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTg > 1:1156878/1‑70 (MQ=255) gcgTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaaa < 1:2190087/71‑1 (MQ=255) ttCCTCGGTGAAGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaa < 1:140809/67‑1 (MQ=255) tCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTg > 1:2327332/1‑71 (MQ=255) cTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGa < 1:2279770/71‑1 (MQ=255) cTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGa < 1:3961642/71‑1 (MQ=255) cTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGa < 1:4411599/71‑1 (MQ=255) tCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTg < 1:758976/68‑1 (MQ=255) cggtgacggtgaAATGGACGAACCGGAATCCAAGGGTGCGATCACCATCGCTACCCGTGaaaaa < 1:3281382/64‑1 (MQ=255) cggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCAcc > 1:444078/1‑46 (MQ=255) cggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaaaa > 1:3966040/1‑64 (MQ=255) cggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaaaa > 1:3752146/1‑64 (MQ=255) ggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCAc > 1:574842/1‑44 (MQ=255) ggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTa > 1:3502890/1‑52 (MQ=255) ggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTa > 1:3973886/1‑52 (MQ=255) ggtgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATa < 1:2950962/70‑1 (MQ=255) tgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAAcc < 1:2387152/71‑1 (MQ=255) tgacggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAAcc < 1:1334078/71‑1 (MQ=255) acggtgaAATGGACGAACCGGAATCCAAAGGTGCGATCa < 1:713460/39‑1 (MQ=255) cggtgaAATGGACGAACCGGAATCCAAGGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTgg < 1:1940013/71‑1 (MQ=255) ggtgaAATGGACGAACCGGAATCCAAGGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGt < 1:1293938/71‑1 (MQ=255) gtgaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATc < 1:2035888/41‑1 (MQ=255) gaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTa > 1:284402/1‑43 (MQ=255) gaAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCtt > 1:4048998/1‑71 (MQ=255) aaaTGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCtt < 1:4508824/70‑1 (MQ=255) aaaTGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCtt < 1:3535088/70‑1 (MQ=255) aTGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAAcc < 1:3885796/61‑1 (MQ=255) aTGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAAc > 1:236252/1‑60 (MQ=255) tGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaa < 1:1485584/48‑1 (MQ=255) tGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaa < 1:1836610/48‑1 (MQ=255) tGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaa < 1:4145782/48‑1 (MQ=255) ggACGAACCGGAATCCAAGGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTa > 1:117240/1‑71 (MQ=255) ggACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGtt < 1:4249742/70‑1 (MQ=255) gACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaaa < 1:62160/47‑1 (MQ=255) gACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGaaaa < 1:2270709/47‑1 (MQ=255) aCGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATc < 1:2529011/71‑1 (MQ=255) cGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATa < 1:3217874/53‑1 (MQ=255) ccGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATc < 1:4107183/66‑1 (MQ=255) cGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTGt < 1:1582987/71‑1 (MQ=255) ggAATCCAAGGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTGTa < 1:2452800/71‑1 (MQ=255) aaTCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTg < 1:3002680/50‑1 (MQ=255) tCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTGTAACCt < 1:1224313/71‑1 (MQ=255) ccAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATa < 1:3710403/42‑1 (MQ=255) ccAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTg < 1:1556551/47‑1 (MQ=255) cAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGt > 1:3544853/1‑54 (MQ=255) aaGGTGCGATCACCATCGCTACCCGTGAAAAACTg < 1:4336009/35‑1 (MQ=255) aaGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTGTAACCTGCAg > 1:3436771/1‑71 (MQ=255) | AAAGATACCTCTAAACAAACCGTTTACGCGTTCCTCGGTGACGGTGAAATGGACGAACCGGAATCCAAAGGTGCGATCACCATCGCTACCCGTGAAAAACTGGATAACCTGGTCTTCGTTATCAACTGTAACCTGCAG > W3110S.gb/123668‑123805 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |