| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,655,114 | C→T | 34.6% | intergenic (‑100/+78) | pepB ← / ← yfhJ | aminopeptidase B/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,655,114 | 0 | C | T | 34.6% | 25.5 / 20.1 | 26 | intergenic (‑100/+78) | pepB/yfhJ | aminopeptidase B/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (9/8); new base T (3/6); total (12/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTG > W3110S.gb/2655051‑2655175 | gCAGCCCCAGGATTTTTCAGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGc < 1:1739042/71‑1 (MQ=255) ccAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAt < 1:891202/71‑1 (MQ=255) ccAGGACTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCa > 1:10001/1‑60 (MQ=255) ttttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCa > 1:4115541/1‑53 (MQ=255) ttttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAg > 1:4305273/1‑54 (MQ=255) tttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAtgt > 1:4164401/1‑71 (MQ=255) ttCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTAtgt < 1:4113747/70‑1 (MQ=255) tCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCAt > 1:2314761/1‑57 (MQ=255) ccGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTATGTGAc > 1:3986958/1‑71 (MQ=255) gCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGa < 1:1168820/50‑1 (MQ=255) gCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGa < 1:3568518/50‑1 (MQ=255) tAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGAt < 1:1882740/56‑1 (MQ=255) aaaTGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTgggg > 1:2326471/1‑71 (MQ=255) aaTGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTATGTGACTgggg > 1:1863704/1‑70 (MQ=255) tGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGt > 1:295079/1‑69 (MQ=255) cgcgTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAg > 1:1460776/1‑37 (MQ=255) gTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgcgc < 1:2026915/71‑1 (MQ=255) gTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAgcgc < 1:730254/71‑1 (MQ=255) tGCAGGAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGATAACTATGTGACTGGGGTGAACGGGCGCAg > 1:4437513/1‑71 (MQ=255) cAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGcc < 1:192596/71‑1 (MQ=255) aGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGcc < 1:187328/70‑1 (MQ=255) gAAGGCGGCAAGTGAGTGAAGCCTCAGGAGCATAGAt < 1:3481228/37‑1 (MQ=255) gAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTAtgt < 1:4147158/45‑1 (MQ=255) aaGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTgggg > 1:3615400/1‑52 (MQ=255) ggcggcAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGc < 1:2774157/64‑1 (MQ=255) agtgaAGCCTCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTg < 1:526524/71‑1 (MQ=255) | GCAGCCCCAGGATTTTTCCGCTAAATGATTCGCGTTGCAGGAAGGCGGCAAGTGAGTGAAGCCCCAGGAGCATAGATAACTATGTGACTGGGGTGAACGAGCGCAGCCAACGCATCTGCGGCGTG > W3110S.gb/2655051‑2655175 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |