Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,682,462 C→G 5.9% A315G (GCC→GGC)  yphH → predicted DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,682,4620CG 5.9% 197.4 / 2.2 67A315G (GCC→GGC) yphHpredicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (33/30);  new base G (2/2);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.61e-01

GCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGT  >  W3110S.gb/2682394‑2682532
                                                                    |                                                                      
gcAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCa                                                                      >  1:1526326/1‑71 (MQ=255)
    ttAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCAt                                                                     <  1:3374771/68‑1 (MQ=255)
     tAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGt                                                                 >  1:1007325/1‑71 (MQ=255)
      aaTCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTTGGTTGATCGCTCTGCCAATGCCATTGGtt                                                                >  1:1900256/1‑71 (MQ=255)
      aaTCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGtt                                                                >  1:593830/1‑71 (MQ=255)
      aaTCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGt                                                                 >  1:78372/1‑70 (MQ=255)
        tCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGttt                                                               >  1:4222055/1‑70 (MQ=255)
          gCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAg                                                            >  1:2190544/1‑71 (MQ=255)
            tGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGc                                                                        >  1:1017743/1‑57 (MQ=255)
            tGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGc                                                                        >  1:831855/1‑57 (MQ=255)
            tGCCTGGCAAAGTGGAGAACCGTGGATCACCACCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGt                                                           <  1:1040125/70‑1 (MQ=255)
              ccTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTg                                                        <  1:4542860/71‑1 (MQ=255)
              ccTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTg                                                        <  1:444437/71‑1 (MQ=255)
              ccTGGCAAAGTGGAGAACCGTGGATCACCAGCTCGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTc                                                          <  1:3646909/69‑1 (MQ=255)
               cTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTg                                                        >  1:1494808/1‑70 (MQ=255)
               cTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGt                                                       <  1:1039690/71‑1 (MQ=255)
                  gCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGtat                                                     <  1:3983928/70‑1 (MQ=255)
                  gCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGtat                                                     >  1:1774324/1‑70 (MQ=255)
                  gCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGtat                                                     >  1:1853172/1‑70 (MQ=255)
                     aaGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACt                                                 >  1:1322982/1‑71 (MQ=255)
                      aGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCAt                                                                     <  1:693923/50‑1 (MQ=255)
                        tGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAg                                                            >  1:869177/1‑57 (MQ=255)
                        tGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTcc                                              >  1:1081753/1‑71 (MQ=255)
                        tGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTACCAATGGCa                                                                      <  1:4526743/47‑1 (MQ=255)
                        tGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTACCAATGGCa                                                                      <  1:2802444/47‑1 (MQ=255)
                          gagaACCGTGGATCACCAGCTGGGTTGATCGCTCTACCAATGGCAtt                                                                    >  1:1919928/1‑47 (MQ=255)
                          gagaACCGTGGATCACCAGCTGGGTTGATCGCTCTACCAATGGCAtt                                                                    >  1:227599/1‑47 (MQ=255)
                               ccGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATTCCATTGGTTTGAGTCTGTATAACTTCCTCAACAt                                       >  1:3109617/1‑71 (MQ=255)
                                  tGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCAtt                                                                    <  1:3134629/39‑1 (MQ=255)
                                  tGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCAtt                                                                    <  1:2863361/39‑1 (MQ=255)
                                   ggATCACCAGCTGGGTTGATCGCTCTGCCAATGCCAtt                                                                    <  1:3207992/38‑1 (MQ=255)
                                   ggATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGa                                                             <  1:3004391/45‑1 (MQ=255)
                                     aTCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTc                                   <  1:2508804/69‑1 (MQ=255)
                                     aTCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTc                                   <  1:1367804/69‑1 (MQ=255)
                                      tCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGttt                                                               <  1:4145909/40‑1 (MQ=255)
                                      tCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGtat                                                     >  1:1088316/1‑50 (MQ=255)
                                      tCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCaa                                 >  1:4541186/1‑70 (MQ=255)
                                       cACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGtt                                                                >  1:1744072/1‑38 (MQ=255)
                                        aCCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCaa                                          >  1:4644083/1‑59 (MQ=255)
                                        aCCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCaa                                          >  1:3487526/1‑59 (MQ=255)
                                        aCCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCaa                                          >  1:75525/1‑59 (MQ=255)
                                         ccAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTTAGTCTGTATAACTTCCTCAACATCCTCaa                                 <  1:668387/67‑1 (MQ=255)
                                         ccAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCaa                                 <  1:4524502/67‑1 (MQ=255)
                                         ccAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAata                               >  1:2019885/1‑69 (MQ=255)
                                         ccAGCTGGGTTGATCACTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTc                                            <  1:635222/56‑1 (MQ=255)
                                          cAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAata                               >  1:448108/1‑68 (MQ=255)
                                           aGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTaa                           >  1:37235/1‑71 (MQ=255)
                                              tGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGt                                                       <  1:4093324/40‑1 (MQ=255)
                                              tGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGt                                                       <  1:1396846/40‑1 (MQ=255)
                                              tGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGt                                                       <  1:2934914/40‑1 (MQ=255)
                                              tGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATaa                                                   >  1:1010485/1‑44 (MQ=255)
                                                  ttGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGAtt                    <  1:3706572/71‑1 (MQ=255)
                                                   tGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCt                                    >  1:1447453/1‑54 (MQ=255)
                                                   tGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCt                                    >  1:3391490/1‑54 (MQ=255)
                                                     aTCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTcc                                              <  1:51645/42‑1 (MQ=255)
                                                       cGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTc                                            >  1:370929/1‑42 (MQ=255)
                                                       cGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAg                       <  1:1595187/63‑1 (MQ=255)
                                                       cGCTCTGCCAATGCCATTGGTTTGAGTATGTATAACTTCCTc                                            >  1:2266268/1‑42 (MQ=255)
                                                        gCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTg              <  1:1262795/71‑1 (MQ=255)
                                                         ctctGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGt             <  1:3958584/71‑1 (MQ=255)
                                                             gCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACgg         >  1:1168965/1‑71 (MQ=255)
                                                              ccAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGt        >  1:2138006/1‑71 (MQ=255)
                                                               cAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTc       >  1:650210/1‑71 (MQ=255)
                                                                aaTGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCa                                  <  1:355238/43‑1 (MQ=255)
                                                                 aTGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTc                                   <  1:4578494/41‑1 (MQ=255)
                                                                 aTGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTc                                   <  1:334154/41‑1 (MQ=255)
                                                                  tGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGa                      <  1:266373/53‑1 (MQ=255)
                                                                   gCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGt                >  1:82138/1‑58 (MQ=255)
                                                                   gCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAg   >  1:2096876/1‑71 (MQ=255)
                                                                    ccATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTc                                   <  1:1277179/38‑1 (MQ=255)
                                                                    ccATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGt  >  1:575863/1‑71 (MQ=255)
                                                                    ccATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGt  <  1:3505121/71‑1 (MQ=255)
                                                                    ccATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGt  <  1:3664823/71‑1 (MQ=255)
                                                                    |                                                                      
GCAGTTAATCGCTGCCTGGCAAAGTGGAGAACCGTGGATCACCAGCTGGGTTGATCGCTCTGCCAATGCCATTGGTTTGAGTCTGTATAACTTCCTCAACATCCTCAATATTAATCAGATTTGGTTGTACGGTCGCAGT  >  W3110S.gb/2682394‑2682532

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: