| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,702,821 | A→G | 29.8% | intergenic (‑102/+170) | rnc ← / ← lepB | RNase III/leader peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,702,821 | 0 | A | G | 29.8% | 62.5 / 35.5 | 47 | intergenic (‑102/+170) | rnc/lepB | RNase III/leader peptidase |
| Reads supporting (aligned to +/- strand): ref base A (17/16); new base G (8/6); total (25/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAAT > W3110S.gb/2702751‑2702887 | cAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGtata > 1:2652385/1‑71 (MQ=255) acacGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATggg < 1:3210263/71‑1 (MQ=255) cacGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATggg > 1:3826318/1‑70 (MQ=255) acGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGa > 1:19888/1‑71 (MQ=255) cGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGAc > 1:120067/1‑71 (MQ=255) cTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTggg < 1:1234197/58‑1 (MQ=255) gATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGa > 1:1126656/1‑58 (MQ=255) taCATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCgtgt > 1:2684904/1‑71 (MQ=255) taCATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCgtgt > 1:401977/1‑71 (MQ=255) tGGTTCTGCTAACTGCTTCGCTACAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCg < 1:3007267/71‑1 (MQ=255) ggTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGt > 1:1431326/1‑71 (MQ=255) gTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGa > 1:4087169/1‑48 (MQ=255) ttCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCgtgt < 1:674453/64‑1 (MQ=255) ttCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTgg > 1:3412103/1‑71 (MQ=255) ttCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTg > 1:3410470/1‑70 (MQ=255) cTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGtata > 1:3870222/1‑39 (MQ=255) cTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGtata > 1:3864909/1‑39 (MQ=255) gCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGa > 1:659473/1‑43 (MQ=255) cTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCa < 1:2180566/68‑1 (MQ=255) cTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCa < 1:4631246/68‑1 (MQ=255) cTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAAc < 1:3263725/70‑1 (MQ=255) tAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGAc > 1:2130889/1‑42 (MQ=255) aaCTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGc > 1:4234329/1‑47 (MQ=255) aaCTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGc > 1:3669142/1‑47 (MQ=255) aCTGCTTCGCTGCAGTTTCAGTTTACCGGTATGTGGGGACCAACGCTg > 1:3743457/1‑48 (MQ=255) aCTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCa < 1:3344772/65‑1 (MQ=255) aCTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTgg < 1:19579/71‑1 (MQ=255) gCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCaa > 1:2122629/1‑40 (MQ=255) cTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGt < 1:3154774/71‑1 (MQ=255) ttCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCa < 1:2462217/60‑1 (MQ=255) ttCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTg > 1:1938250/1‑71 (MQ=255) cGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACg > 1:1593209/1‑38 (MQ=255) gCTGCAGTTTCAGTTTACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGcc < 1:1928866/70‑1 (MQ=255) gCTGCAGTTTCAGTTTACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGcc < 1:4283138/70‑1 (MQ=255) cTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCaa < 1:1200201/71‑1 (MQ=255) cagtttcagtttACCGGTATGTGGGGACCACCGCTg < 1:4191401/36‑1 (MQ=37) gtttcagtttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTa < 1:3874543/71‑1 (MQ=255) gtttcagtttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTa < 1:1668151/71‑1 (MQ=255) tttcagtttACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTa > 1:1226574/1‑70 (MQ=255) tttcagtttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAAc > 1:3770044/1‑52 (MQ=255) tcagtttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAAc > 1:459094/1‑71 (MQ=255) agtttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCa < 1:3892941/46‑1 (MQ=255) tttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGt < 1:3760177/40‑1 (MQ=255) tttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGt < 1:418210/40‑1 (MQ=255) tttACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTgg < 1:4628024/50‑1 (MQ=255) ttACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAAc > 1:3324606/1‑45 (MQ=255) ttACCGGTATGTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAAc > 1:1991735/1‑45 (MQ=255) tACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAAc > 1:1775020/1‑44 (MQ=255) tACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGa > 1:90305/1‑49 (MQ=255) gTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAat < 1:2623887/71‑1 (MQ=255) tatgTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAat > 1:4618800/1‑70 (MQ=255) | CAGCACACGAAACAGACCTGATATACATGGTTCTGCTAACTGCTTCGCTGCAGTTTCAGTTTACCGGTATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAAT > W3110S.gb/2702751‑2702887 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |