| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,703,823 | T→A | 52.7% | K48I (AAA→ATA) | lepB ← | leader peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,703,823 | 0 | T | A | 52.7% | ‑1.8 / 51.9 | 36 | K48I (AAA→ATA) | lepB | leader peptidase |
| Reads supporting (aligned to +/- strand): ref base T (11/6); new base A (15/4); total (26/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
CCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAA > W3110S.gb/2703758‑2703888 | ccGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATc < 1:1938224/70‑1 (MQ=255) gAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGt < 1:335402/71‑1 (MQ=255) gAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGt < 1:2628105/71‑1 (MQ=255) aGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTccc > 1:2866524/1‑71 (MQ=255) ccGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTAt > 1:173024/1‑52 (MQ=255) tttCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAgccgcc < 1:2577520/71‑1 (MQ=255) ccagccagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAg > 1:2642587/1‑45 (MQ=255) ccagccagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTg > 1:461772/1‑66 (MQ=255) cagccagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGa > 1:3025732/1‑47 (MQ=255) cagGCTTCGGCGCAACCTTTTTCAACGTTGCTTTa > 1:2057034/1‑35 (MQ=255) gCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGa > 1:3376315/1‑71 (MQ=255) ttCGGCGCAACCTTTTTCAACGTTGCTTTATCCAg > 1:1716668/1‑35 (MQ=255) ttCGGCGCAACCTTTTTCAACGTTGCTTTATCCAg > 1:2314694/1‑35 (MQ=255) cAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCccgc > 1:405055/1‑71 (MQ=255) aCCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGcgcc < 1:1211798/51‑1 (MQ=255) tttCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTa < 1:2213933/70‑1 (MQ=255) tatcaACGGTGGTATATCCAGTGAtttttttctccatttt < 1:3576562‑M1/38‑17 (MQ=255) tatcaACGGTGGTATATCCAGTGAtttttttctccatttt < 1:1725138‑M1/38‑17 (MQ=255) atcAACGGTGGTATATCCAGTGAtttttttctccatt > 1:4402812‑M1/2‑23 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccattttag > 1:745773‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccattttag > 1:3767928‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccattttag > 1:3593813‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccattttag > 1:2926478‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccattttag > 1:284293‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccatttta > 1:235712‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccatttta > 1:514671‑M1/1‑22 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccatttt < 1:2693876‑M1/38‑17 (MQ=255) tCAACGGTGGTATATCCAGTGAtttttttctccattt > 1:2142266‑M1/1‑22 (MQ=255) cAACGGTGGTATATCCAGTGAtttttttctccatttt < 1:1758723‑M1/37‑17 (MQ=255) cAACGGTGGTATATCCAGTGAtttttttctccattt > 1:380552‑M1/1‑21 (MQ=255) cAACGGTGGTATATCCAGTGAtttttttctccattt > 1:4551515‑M1/1‑21 (MQ=255) cAACGGTGGTATATCCAGTGAtttttttctccattt > 1:4556899‑M1/1‑21 (MQ=255) aaCGGTGGTATATCCAGTGAtttttttctccatttt > 1:3218658‑M1/1‑20 (MQ=255) aaCGGTGGTATATCCAGTGAtttttttctccatttt > 1:2134357‑M1/1‑20 (MQ=255) aaCGGTGGTATATCCAGTGAtttttttctccatttt > 1:2547131‑M1/1‑20 (MQ=255) gCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGaaa > 1:3805914/1‑69 (MQ=255) | CCGGAAAAACAGAAGCACCGGTTTCCAGCCAGCCAGGCTTCGGCGCAACCTTTTTCAACGTTGCTTTATCCAGTGAGTCCCCGGCAGCCGCCTGCGCCGCTGCCTGACGTTCCCGCCGTTTAGGTGCGAAA > W3110S.gb/2703758‑2703888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |