| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,720,043 | A→G | 11.5% | T479A (ACG→GCG) | yfiQ → | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,720,043 | 0 | A | G | 11.5% | 154.1 / 12.2 | 61 | T479A (ACG→GCG) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (22/32); new base G (4/3); total (26/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.98e-01 | |||||||||||
GCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACC > W3110S.gb/2719973‑2720108 | gCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTa > 1:1684385/1‑71 (MQ=255) cccAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTAc < 1:1910750/71‑1 (MQ=255) cccAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTAc < 1:3731281/71‑1 (MQ=255) cAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGc > 1:2043424/1‑71 (MQ=255) aTCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTc < 1:3805407/71‑1 (MQ=255) cTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCCCGa > 1:2274165/1‑71 (MQ=255) tGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTc < 1:744347/68‑1 (MQ=255) tGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCACGa > 1:422337/1‑70 (MQ=255) gACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGa < 1:2301279/69‑1 (MQ=255) ttCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTc < 1:2673944/64‑1 (MQ=255) ccAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCg > 1:4648525/1‑63 (MQ=255) cAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGa > 1:1008324/1‑63 (MQ=255) cAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGAt > 1:3810550/1‑64 (MQ=255) cAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATg < 1:2973801/71‑1 (MQ=255) tACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTAc < 1:3688472/51‑1 (MQ=255) tACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAg > 1:568335/1‑71 (MQ=255) aCCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGt > 1:4240193/1‑71 (MQ=255) aCCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGt > 1:4354997/1‑71 (MQ=255) ccGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGAt > 1:38924/1‑59 (MQ=255) ccGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGtt > 1:4490216/1‑71 (MQ=255) gCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCa < 1:3907805/71‑1 (MQ=255) gCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCa < 1:1349805/71‑1 (MQ=255) cAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTGCGTCGCTCGATACCCATGAAGTTCAg > 1:536098/1‑71 (MQ=255) tcttctGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATAc < 1:4642788/48‑1 (MQ=255) cttctGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCGGCCCATCCTGc > 1:3844814/1‑70 (MQ=255) tctGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAg < 1:1553098/58‑1 (MQ=255) ctGTTGCAACAGGCGATTGCCGAAGGGGCTGCGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGc < 1:1807382/67‑1 (MQ=255) ctGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGc > 1:2552475/1‑71 (MQ=255) gTTGCAACAGGCGATTGCCGAAGGGGCTGCGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCg > 1:1284406/1‑70 (MQ=255) ttGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATg < 1:2176277/46‑1 (MQ=255) ttGCAACAGGCGATTCCCGAAGGGGCTACGTCGCTCGATACCCATg < 1:3295797/46‑1 (MQ=255) gCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGaa > 1:311571/1‑46 (MQ=255) gCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATcc > 1:1363767/1‑59 (MQ=255) cAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATg < 1:2268093/70‑1 (MQ=255) cAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATg < 1:3464279/70‑1 (MQ=255) aaCAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGc < 1:3619103/71‑1 (MQ=255) aCAGGCGATTGCCGAAGGGGCTACATCGCTCGATACCCATGAAGtt < 1:2518618/46‑1 (MQ=255) cAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCg < 1:4587959/63‑1 (MQ=255) aGGCGATTGCCGAAGGGGCTGCGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCAt > 1:3163640/1‑70 (MQ=255) aGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCa < 1:4204678/46‑1 (MQ=255) aGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATg < 1:3390237/71‑1 (MQ=255) aGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATg < 1:2129389/71‑1 (MQ=255) ggCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATg < 1:1097476/38‑1 (MQ=255) ggCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAGGCGTATGGCATg > 1:3903197/1‑70 (MQ=255) ggCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGa < 1:622255/71‑1 (MQ=255) gATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTa < 1:3198758/60‑1 (MQ=255) gATTGCCAAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAaca < 1:2807802/71‑1 (MQ=255) aTTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCATCCCATCCTGCAAGCGTATGGCATGAacac < 1:1358034/71‑1 (MQ=255) ttGCCGAAGGGGCTGCGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACg > 1:1341782/1‑71 (MQ=255) ttGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCg < 1:2695554/56‑1 (MQ=255) ttGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACg > 1:1048023/1‑71 (MQ=255) tGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTa > 1:481275/1‑57 (MQ=255) aaGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATc > 1:1772812/1‑41 (MQ=255) aaGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCt < 1:1660949/71‑1 (MQ=255) gggCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATg < 1:3473173/56‑1 (MQ=255) gggCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTAcc > 1:3431056/1‑71 (MQ=255) gggCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTAcc > 1:3425497/1‑71 (MQ=255) gCTGCGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATgg < 1:1588925/50‑1 (MQ=255) gCTGCGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATgg < 1:784820/50‑1 (MQ=255) gCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGt < 1:3918522/46‑1 (MQ=255) gCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCa > 1:3080124/1‑52 (MQ=255) tACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAac < 1:3007492/55‑1 (MQ=255) | GCCCAGCAATCTGACTTCCAATACCGCAGAAGCGCATCTTCTGTTGCAACAGGCGATTGCCGAAGGGGCTACGTCGCTCGATACCCATGAAGTTCAGCCCATCCTGCAAGCGTATGGCATGAACACGCTCCCTACC > W3110S.gb/2719973‑2720108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |