| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,720,297 | G→A | 27.5% | W563* (TGG→TGA) | yfiQ → | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,720,297 | 0 | G | A | 27.5% | 62.2 / 25.5 | 40 | W563* (TGG→TGA) | yfiQ | fused predicted acyl‑CoA synthetase NAD(P)‑binding subunit and ATP‑binding subunit |
| Reads supporting (aligned to +/- strand): ref base G (14/15); new base A (4/7); total (18/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01 | |||||||||||
CAGCCAATGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAGGAGTT > W3110S.gb/2720239‑2720362 | cAGCCAATGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCgg > 1:4206058/1‑71 (MQ=255)cAGCCAATGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCgg > 1:4076739/1‑71 (MQ=255)cAGCCAATGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCgg > 1:2486088/1‑71 (MQ=255) cAATGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTc < 1:368351/70‑1 (MQ=255) aaTGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCa < 1:3554483/71‑1 (MQ=255) tGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACg > 1:3577514/1‑71 (MQ=255) gTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCt > 1:2668376/1‑71 (MQ=255) tCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCACGGCCTg < 1:2928439/71‑1 (MQ=255) cAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGtt > 1:107346/1‑71 (MQ=255) cAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGtt > 1:3267634/1‑71 (MQ=255) cAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGt < 1:3813934/70‑1 (MQ=255) gCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTgg < 1:2236812/71‑1 (MQ=255) gCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCACGGCCTGt > 1:3471797/1‑68 (MQ=255) cAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTgg < 1:1185610/70‑1 (MQ=255) cAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGt > 1:776345/1‑71 (MQ=255) gcggcgAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCa < 1:894989/71‑1 (MQ=255) gcgAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCTCGGGTCCACGGCCTGTTGGTGCAAAg < 1:4217985/71‑1 (MQ=255) cgAACGCTATTTTCGATCGCGTAAAAAtggcctggcc < 1:1595188/37‑1 (MQ=255) cgAACGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCa > 1:4008453/1‑52 (MQ=255) cgAACGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCa > 1:4003175/1‑52 (MQ=255) aaCGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTAt < 1:936169/71‑1 (MQ=255) aaCGCTATTTTCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTAt < 1:4084293/71‑1 (MQ=255) cTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTc < 1:3905658/44‑1 (MQ=255) tATTTTCGATCGCGTAAAAATGGCCTGGGCACAGGCGCGGGTCCACGGCCTGTTGGTGCaa > 1:345216/1‑61 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACgg > 1:2246197/1‑46 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCaa < 1:1133937/59‑1 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTa > 1:3370274/1‑63 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTaa < 1:3218885/70‑1 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTa < 1:705581/69‑1 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAAc > 1:1907304/1‑71 (MQ=255) ttttCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCACGGCCTGTTg < 1:4339248/53‑1 (MQ=255) ttCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTc < 1:942176/39‑1 (MQ=255) ttCGATCGCGTAAAAATGGCCTGACCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTAt < 1:2767395/62‑1 (MQ=255) tCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCa > 1:2690862/1‑40 (MQ=255) aTCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCg < 1:67572/67‑1 (MQ=255) tCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTg > 1:3259818/1‑71 (MQ=255) tAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCCAACCGTGCTGGCGCt < 1:2484327/71‑1 (MQ=255) aaTGGCCTGACCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAg > 1:1166135/1‑70 (MQ=255) aTGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAgg < 1:346156/70‑1 (MQ=255) aTGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAgg < 1:3018697/70‑1 (MQ=255) aTGGCCTGACCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAg < 1:4630329/44‑1 (MQ=255) tggcctggccACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAGGa > 1:3208071/1‑70 (MQ=255) gcctggccACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAGGAGtt > 1:828550/1‑71 (MQ=255) | CAGCCAATGAAGTCCAGCAAGCGGCGAACGCTATTTTCGATCGCGTAAAAATGGCCTGGCCACAGGCGCGGGTCCACGGCCTGTTGGTGCAAAGTATGGCTAACCGTGCTGGCGCTCAGGAGTT > W3110S.gb/2720239‑2720362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |