| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 131,249 | A→G | 24.4% | T4T (ACT→ACC) | yacH ← | hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 131,249 | 0 | A | G | 24.4% | 70.8 / 23.3 | 41 | T4T (ACT→ACC) | yacH | hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/11); new base G (6/4); total (26/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01 | |||||||||||
CCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCGCAGGTGCTG > W3110S.gb/131183‑131315 | cccGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAGGTCa < 1:1983813/71‑1 (MQ=255) gTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCa < 1:2409453/67‑1 (MQ=255) gTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAtttt > 1:1608871/1‑71 (MQ=255) gTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAttt > 1:1501443/1‑70 (MQ=255) ggcggcACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATaa > 1:3309593/1‑71 (MQ=255) cacaAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAt > 1:457083/1‑58 (MQ=255) caAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGTTTTAAACGGCAAAGTCATTTTCATaa < 1:2600967/64‑1 (MQ=255) caAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGAt > 1:3162813/1‑71 (MQ=255) caAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGAt > 1:4253214/1‑71 (MQ=255) aaGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGAtct > 1:1988736/1‑71 (MQ=255) gCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGaa < 1:3485493/71‑1 (MQ=255) cTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCAttt < 1:3124116/46‑1 (MQ=255) cAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAGGTCATTTTCATAATTCGGATCTCAAGGAAATCGc > 1:2340560/1‑70 (MQ=255) cAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGc < 1:1830143/70‑1 (MQ=255) aaTTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAAt > 1:1005966/1‑71 (MQ=255) aTTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGc < 1:1424958/67‑1 (MQ=255) ttAGCGCCAGCACATGGGGTTTAAACGGCAAAGTc < 1:1280593/35‑1 (MQ=255) ttAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAAt > 1:2872647/1‑63 (MQ=255) ccAGCACATGGGGTTTAAACGGCAAGGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCg < 1:2550556/71‑1 (MQ=255) ccAGCACATGGGGTTTAAACGGCAAGGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCg < 1:2898785/71‑1 (MQ=255) ccAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCg < 1:1595108/71‑1 (MQ=255) aGCACATGGGGTTTAAACGGCAAGGTCATTTTCATAATTCGGATCTCAAGGAAAt > 1:4541870/1‑55 (MQ=255) gCACATGGGGTTTAAACGGCAAAGTCATTTTCATAAt > 1:3296664/1‑37 (MQ=255) caTGGGGTTTAAACGGCAAGGTCATTTTCATAATTCGGATCTCAAg < 1:3821693/46‑1 (MQ=255) caTGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCg < 1:4549031/65‑1 (MQ=255) gggTTTAAACGGCAAAGTCATTTTCATAATTCGGAt > 1:1178078/1‑36 (MQ=255) ttAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGaaa > 1:2098117/1‑42 (MQ=255) tAAACGGCAAGGTCATTTTCATAATTCGGATCTCAAGGaaa > 1:4078345/1‑41 (MQ=255) tAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGaa > 1:927817/1‑40 (MQ=255) tAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCGCAg > 1:3674618/1‑71 (MQ=255) aaaaCGGCAAAGTCATTTTCATAATTCGGATCTCAAGGaa > 1:1207365/2‑40 (MQ=255) aaCGGCAAGGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCGCAGGt > 1:3679806/1‑71 (MQ=255) aaCGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGt > 1:3396890/1‑49 (MQ=255) aaCGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTg > 1:1570882/1‑59 (MQ=255) aaCGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCGCAGGt < 1:73985/71‑1 (MQ=255) cGGCAAGGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCAcc > 1:4118489/1‑62 (MQ=255) cGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCg < 1:3508797/64‑1 (MQ=255) gCAAGGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAAc > 1:3980954/1‑53 (MQ=255) gCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCGCAGGTGCtg > 1:1184970/1‑71 (MQ=255) cAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGaa > 1:2804852/1‑51 (MQ=255) cAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGaa > 1:1400138/1‑51 (MQ=255) | CCCGGTAGAGGCGGCACAAAGCCCGGCACTGCAAATTAGCGCCAGCACATGGGGTTTAAACGGCAAAGTCATTTTCATAATTCGGATCTCAAGGAAATCGCAATGGTCGGCGAACTGCCACCCGCAGGTGCTG > W3110S.gb/131183‑131315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |