| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,729,684 | T→C | 32.5% | noncoding (130/1542 nt) | rrsG ← | 16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,729,684 | 0 | T | C | 32.5% | 44.7 / 33.8 | 40 | noncoding (130/1542 nt) | rrsG | 16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base T (18/9); new base C (5/8); total (23/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.05e-01 | |||||||||||
CTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAC > W3110S.gb/2729614‑2729753 | ctctTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCt > 1:2406617/1‑71 (MQ=38)ctctTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCt > 1:1731388/1‑71 (MQ=38) tttGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTcc > 1:3181789/1‑71 (MQ=39) tttGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTcc > 1:3296132/1‑71 (MQ=39) ttGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTcc > 1:757569/1‑70 (MQ=40) gTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCa < 1:3533410/69‑1 (MQ=255) cTTGCGACATTATGAGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACa > 1:1376172/1‑71 (MQ=255) tGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACAt > 1:2246404/1‑70 (MQ=40) gCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACAt < 1:1047445/69‑1 (MQ=40) gCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCAGACAtt < 1:3022790/70‑1 (MQ=255) gACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACt < 1:2149117/71‑1 (MQ=39) gACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCAGACATTACt > 1:2389305/1‑71 (MQ=255) ttATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTc < 1:2796347/68‑1 (MQ=35) aTGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCACCCg > 1:3016093/1‑71 (MQ=21) gTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGa < 1:4333395/53‑1 (MQ=35) ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGa < 1:4625322/50‑1 (MQ=38) ttAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCa < 1:3923559/70‑1 (MQ=25) tACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGt > 1:465173/1‑70 (MQ=35) tCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAag > 1:885734/1‑71 (MQ=35) tagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca < 1:4445537/71‑1 (MQ=39) tagtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca < 1:4061582/71‑1 (MQ=39) gtTATCCCCCTCCATCAGTCAGCCTCCCAGACATTAc < 1:106945/37‑1 (MQ=14) gtTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg > 1:1772905/1‑71 (MQ=39) ttATCCCCCTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgc < 1:1653190/71‑1 (MQ=255) aTCCCCCTCCATCAGGCAGGCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgct < 1:1609869/70‑1 (MQ=255) aTCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgct > 1:3494825/1‑70 (MQ=40) cccccTCCATCAGGCAGCCTCCCAGACATTACTCAcc > 1:1916750/1‑37 (MQ=255) cccccTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAg > 1:1911320/1‑54 (MQ=255) cccccTCCATCAGGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAgctgct > 1:1167596/1‑71 (MQ=255) cTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACt > 1:2219382/1‑44 (MQ=38) cTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCt < 1:3309452/71‑1 (MQ=255) tCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGt > 1:4391175/1‑46 (MQ=38) tCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAgcagca > 1:2152946/1‑59 (MQ=255) aTCAGGCAGCTTCCCAGACATTACTCACCCGTCCg > 1:3082053/1‑35 (MQ=40) tCATGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGt < 1:4207818/68‑1 (MQ=255) tCATGCAGCCTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGt < 1:4212974/68‑1 (MQ=255) tCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCGACTCGTCAGCGAAGCAGCAAg > 1:2920237/1‑57 (MQ=255) tCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGt > 1:1649721/1‑68 (MQ=255) aGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCg > 1:3343567/1‑71 (MQ=255) ggCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAg > 1:2926429/1‑54 (MQ=255) aGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTg > 1:3315868/1‑61 (MQ=255) cTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAc < 1:751431/71‑1 (MQ=40) | CTCTTTGGTCTTGCGACATTATGCGGTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCAGCTTCCCAGACATTACTCACCCGTCCGCCACTCGTCAGCGAAGCAGCAAGCTGCTTCCTGTTACCGTTCGAC > W3110S.gb/2729614‑2729753 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |