Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,750,169 C→T 17.3% T228M (ACG→ATG)  yfjB → NAD kinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,750,1690CT17.3% 184.3 / 27.7 75T228M (ACG→ATG) yfjBNAD kinase
Reads supporting (aligned to +/- strand):  ref base C (39/23);  new base T (6/7);  total (45/30)
Fisher's exact test for biased strand distribution p-value = 3.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCC  >  W3110S.gb/2750100‑2750238
                                                                     |                                                                     
cGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCAc                                                                       >  1:2478758/1‑70 (MQ=255)
 gATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGa                                                                     <  1:149700/71‑1 (MQ=255)
  aTTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGAt                                                                    <  1:3247981/71‑1 (MQ=255)
    tACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATcc                                                                  >  1:3986637/1‑71 (MQ=255)
      cccTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGt                                                                >  1:2983380/1‑71 (MQ=255)
         tGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTg                                                             >  1:3275894/1‑71 (MQ=255)
         tGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTg                                                             >  1:4366739/1‑71 (MQ=255)
          ggTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGc                                                            >  1:883199/1‑71 (MQ=255)
          ggTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGc                                                            >  1:2802106/1‑71 (MQ=255)
          ggTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGc                                                            >  1:1742197/1‑71 (MQ=255)
          ggTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGc                                                            >  1:174642/1‑71 (MQ=255)
           gTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCg                                                           >  1:4497355/1‑71 (MQ=255)
           gTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCg                                                           <  1:2260113/71‑1 (MQ=255)
              cccATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGttt                                                        <  1:3269413/71‑1 (MQ=255)
              cccATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGttt                                                        >  1:2345695/1‑71 (MQ=255)
              cccATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGttt                                                        <  1:3027688/71‑1 (MQ=255)
              cccATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGttt                                                        <  1:4113184/71‑1 (MQ=255)
               ccATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGttt                                                        <  1:2317293/70‑1 (MQ=255)
                    ttCCCGCATGCGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGc                                                   <  1:1851691/70‑1 (MQ=255)
                    ttCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                                                  >  1:4210811/1‑71 (MQ=255)
                    ttCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                                                  >  1:2628405/1‑71 (MQ=255)
                    ttCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                                                  >  1:1133376/1‑71 (MQ=255)
                    ttCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCa                                                  >  1:3893255/1‑71 (MQ=255)
                      cccGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCAt                                                 <  1:1447999/70‑1 (MQ=255)
                      cccGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATcc                                                                  >  1:559123/1‑53 (MQ=255)
                       ccGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCg                                               <  1:3832906/71‑1 (MQ=255)
                       ccGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATc                                                <  1:111776/70‑1 (MQ=255)
                        cGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGc                                              >  1:3931352/1‑71 (MQ=255)
                          cATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATcc                                                                  <  1:1288478/49‑1 (MQ=255)
                          cATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                                            >  1:1333241/1‑71 (MQ=255)
                          cATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCg                                            >  1:1848343/1‑71 (MQ=255)
                           aTACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt                                           <  1:3661210/71‑1 (MQ=255)
                            tACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGt                                                                >  1:3296925/1‑49 (MQ=255)
                            tACGTTGTCAGAACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt                                           >  1:1602564/1‑70 (MQ=255)
                             aCGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa                                         >  1:3829882/1‑71 (MQ=255)
                             aCGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa                                         >  1:3854070/1‑71 (MQ=255)
                              cGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTAAc                                        >  1:3455780/1‑71 (MQ=255)
                               gTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa                                         <  1:3801223/69‑1 (MQ=255)
                               gTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACg                                       <  1:2505983/71‑1 (MQ=255)
                                ttGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGttttt                                                      <  1:2041716/55‑1 (MQ=255)
                                  gTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATc                                                                   >  1:3792925/1‑40 (MQ=255)
                                  gTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATc                                                                   >  1:2746386/1‑40 (MQ=255)
                                   tCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGttt                                                        <  1:3089499/50‑1 (MQ=255)
                                     aGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGt                                                                >  1:951532/1‑40 (MQ=255)
                                        aCGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGaaa                              >  1:3617872/1‑71 (MQ=255)
                                         cGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAt                             >  1:3255332/1‑71 (MQ=255)
                                          gACCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc                            <  1:3893662/71‑1 (MQ=255)
                                          gACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATc                            <  1:1742077/71‑1 (MQ=255)
                                           aCCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTaa                                         >  1:2219669/1‑57 (MQ=255)
                                           aCCACTGGTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTaa                                         >  1:2832427/1‑57 (MQ=255)
                                           aCCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAAt                             >  1:180873/1‑69 (MQ=255)
                                            ccACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAg                          <  1:1072315/71‑1 (MQ=255)
                                              aCTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                                                 >  1:3543001/1‑46 (MQ=255)
                                               cTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGc                                                   >  1:3674726/1‑43 (MQ=255)
                                               cTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACg                                       <  1:1839720/55‑1 (MQ=255)
                                                tGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACg                                       <  1:3318797/54‑1 (MQ=255)
                                                  gTCATAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGc                      <  1:3648338/69‑1 (MQ=255)
                                                   tCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGAc                   <  1:4275613/71‑1 (MQ=255)
                                                    cATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGa                    >  1:1091929/1‑69 (MQ=255)
                                                     aTAAACAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTg                                  >  1:2616107/1‑54 (MQ=255)
                                                     aTAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAg                 <  1:117937/71‑1 (MQ=255)
                                                      tAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCAt                                                 >  1:719580/1‑38 (MQ=255)
                                                      tAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGt                                           <  1:2106405/44‑1 (MQ=255)
                                                       aaaCAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTaa                                         >  1:2060792/1‑45 (MQ=255)
                                                       aaaCAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGc                >  1:3130925/1‑70 (MQ=255)
                                                        aaCAGCAGCAGCATGATCCGTCTGCGTTTTTCGCATCGCCGTAACGa                                      >  1:3113734/1‑47 (MQ=255)
                                                        aaCAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCt                                   >  1:533173/1‑50 (MQ=255)
                                                          cagcagcagCACGATCCGTCTGCGTTTTTCGCATCGcc                                             >  1:2810689/1‑38 (MQ=255)
                                                             cagcagcaCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAg         <  1:4119628/71‑1 (MQ=255)
                                                              agcagcaCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTgg                                 <  1:1509551/46‑1 (MQ=255)
                                                              agcagcaCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCa                           >  1:2117578/1‑52 (MQ=255)
                                                              agcagcaCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAg         <  1:941052/70‑1 (MQ=255)
                                                               gcagcaCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAg             <  1:1534562/65‑1 (MQ=255)
                                                                  gcaCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGa            >  1:3558363/1‑63 (MQ=255)
                                                                    aCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCAc      >  1:2709774/1‑67 (MQ=255)
                                                                    aCGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGcc  >  1:3700462/1‑71 (MQ=255)
                                                                     |                                                                     
CGATTACCCTGGTGCCCATGTTCCCGCATACGTTGTCAGCACGACCACTGGTCATAAACAGCAGCAGCACGATCCGTCTGCGTTTTTCGCATCGCCGTAACGACCTGGAAATCAGTTGCGACAGCCAGATAGCACTGCC  >  W3110S.gb/2750100‑2750238

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑