| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,764,566 | T→A | 8.3% | intergenic (‑134/‑8) | yfjM ← / → yfjN | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,764,566 | 0 | T | A | 8.3% | 160.0 / 5.1 | 59 | intergenic (‑134/‑8) | yfjM/yfjN | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (28/26); new base A (2/3); total (30/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
AAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATC > W3110S.gb/2764499‑2764632 | aaaTTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc > 1:864360/1‑69 (MQ=255) aTTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAg > 1:348441/1‑70 (MQ=255) aTTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGAACCAgg < 1:1152222/71‑1 (MQ=255) ttAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGa < 1:3932157/70‑1 (MQ=255) cAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGa < 1:1330101/71‑1 (MQ=255) aaaTCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGAc < 1:349625/71‑1 (MQ=255) aTACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAg < 1:3324485/57‑1 (MQ=255) aTACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg < 1:3947942/71‑1 (MQ=255) acacTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg > 1:3369940/1‑69 (MQ=255) acTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGt < 1:547738/71‑1 (MQ=255) ggggTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaaaa > 1:3947698/1‑71 (MQ=255) tattatTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTa < 1:2618766/71‑1 (MQ=255) ttattGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg > 1:2469553/1‑53 (MQ=255) ttattGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGTAg < 1:3319068/56‑1 (MQ=255) ttattGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaa < 1:12627/63‑1 (MQ=255) ttattGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAAc > 1:1433198/1‑66 (MQ=255) ttattGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAAc > 1:4174494/1‑66 (MQ=255) ttattGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaa < 1:1164771/70‑1 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGaaa > 1:2313313/1‑41 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt > 1:1580071/1‑48 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAAt > 1:3974638/1‑48 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg > 1:979994/1‑51 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg < 1:347621/51‑1 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAg > 1:2035024/1‑51 (MQ=255) attGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGa > 1:1222450/1‑53 (MQ=255) attGTAGAGTTTCCCCATATCTTTCTATGGGATCCAGGAAATGAc > 1:1104552/1‑45 (MQ=255) gTAGAGTTTCCCCATATGTTTCTATGGGAACCAGGAAATGACAATCAGGAGTTACAAAAACTTaaa > 1:578290/1‑66 (MQ=255) tAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt < 1:4513440/71‑1 (MQ=255) tAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt < 1:4408794/71‑1 (MQ=255) agagTTTCCCCATATGTTTCTATGGGAACCAGGAAATGACa > 1:2412382/1‑41 (MQ=255) gagTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt > 1:1952995/1‑69 (MQ=255) gagTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGt > 1:812205/1‑69 (MQ=255) tttCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTa < 1:787460/48‑1 (MQ=255) tttCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaa < 1:4130907/52‑1 (MQ=255) tttCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaa < 1:3795336/59‑1 (MQ=255) tCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc > 1:4544275/1‑70 (MQ=255) tCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGc > 1:2293240/1‑70 (MQ=255) ccATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCaaa > 1:3385572/1‑70 (MQ=255) cATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAg > 1:1813652/1‑39 (MQ=255) atatGTTTCTATGTGATCCAGGAAATGACAATCAGGAGTTACaaa < 1:368846/45‑1 (MQ=255) atatGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAatat > 1:2851014/1‑71 (MQ=255) tatGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaa < 1:4608556/44‑1 (MQ=255) tatGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCa < 1:2737262/65‑1 (MQ=255) tatGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATc < 1:2831346/71‑1 (MQ=255) atGTTTCTATGGGAACCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATc < 1:2924340/53‑1 (MQ=255) tGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt < 1:3816455/46‑1 (MQ=255) tGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATc > 1:2959119/1‑69 (MQ=255) tGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATc > 1:2447009/1‑69 (MQ=255) tGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATc > 1:2306919/1‑69 (MQ=255) gTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACt < 1:4062275/45‑1 (MQ=255) tttCTATGGGAACCAGGAAATGACAATCAGGAGTTACAAAAACTTaa < 1:2406130/47‑1 (MQ=255) ttCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAAt > 1:3896845/1‑48 (MQ=255) tCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaa < 1:3753236/45‑1 (MQ=255) tCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCa > 1:1208576/1‑54 (MQ=255) tCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCa > 1:2447735/1‑54 (MQ=255) tCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACt > 1:2159394/1‑71 (MQ=255) tGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTg < 1:674810/68‑1 (MQ=255) ggATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATc < 1:1673269/70‑1 (MQ=255) gATCCAGGAAATGACAATCAGGAGTTACAAAAACTTaa < 1:2672/38‑1 (MQ=255) | AAATTTAGCAAATCAATACACTTCAGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAAAACTTAAATCTGGTCAGGGCAAATATCGAGACTGAATC > W3110S.gb/2764499‑2764632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |