| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,813,784 | C→T | 8.8% | A438T (GCC→ACC) | gshA ← | gamma‑glutamate‑cysteine ligase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,813,784 | 0 | C | T | 8.8% | 208.4 / 8.4 | 68 | A438T (GCC→ACC) | gshA | gamma‑glutamate‑cysteine ligase |
| Reads supporting (aligned to +/- strand): ref base C (29/33); new base T (4/2); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.21e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATACCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGC > W3110S.gb/2813717‑2813847 | aTACCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAAc > 1:1232688/1‑71 (MQ=255) aCCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAAc > 1:3480821/1‑69 (MQ=255) aCCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAAc > 1:3700910/1‑69 (MQ=255) aGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAg > 1:499535/1‑69 (MQ=255) gTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAAc < 1:4113313/65‑1 (MQ=255) tATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtca < 1:1393388/71‑1 (MQ=255) tcaatcaTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGTAACCAGTtcat > 1:2410408/1‑70 (MQ=255) tcaatcaTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtcat < 1:2936062/70‑1 (MQ=255) tcaatcaTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtcat < 1:4534969/70‑1 (MQ=255) tcaTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtcat < 1:3491466/66‑1 (MQ=255) tcaTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtcat < 1:1350680/66‑1 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGTAACCAGTTCATcacaca < 1:4118083/70‑1 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGtt > 1:2253505/1‑61 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtcatca > 1:898482/1‑66 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCacacac > 1:1571799/1‑71 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCacacac > 1:400307/1‑71 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCacacac > 1:909754/1‑71 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCacacac > 1:915008/1‑71 (MQ=255) aTAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCacacac > 1:3083066/1‑71 (MQ=255) tAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACt < 1:3265008/71‑1 (MQ=255) cTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtca < 1:4134527/57‑1 (MQ=255) aaGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtca > 1:976642/1‑54 (MQ=255) aaGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGa < 1:2840441/69‑1 (MQ=255) aaGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGa < 1:843049/69‑1 (MQ=255) aaGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATa > 1:1335520/1‑71 (MQ=255) aGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATa < 1:354498/70‑1 (MQ=255) tACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAg > 1:1259528/1‑46 (MQ=255) tACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATAACACACTTTCTGATACGCt < 1:3655464/71‑1 (MQ=255) aCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCtt > 1:4462133/1‑71 (MQ=255) aCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCtt > 1:1320935/1‑71 (MQ=255) aCGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCtt > 1:3242440/1‑71 (MQ=255) cGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTc < 1:4398829/71‑1 (MQ=255) cAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTcgccg > 1:951558/1‑71 (MQ=255) cAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCCGATACGCTTcgccg > 1:3348277/1‑71 (MQ=255) agagAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATcac < 1:465419/47‑1 (MQ=255) gagaAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTcgcc < 1:1189033/68‑1 (MQ=255) agaAAGTCAGATCGGGATTATCGAAGCAGGCAACCa > 1:1760953/1‑36 (MQ=255) agaAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGtt < 1:1164829/39‑1 (MQ=255) agaAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGtt < 1:547020/39‑1 (MQ=255) agaAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGtt < 1:2843124/39‑1 (MQ=255) gaAAGTCAGATCGGGATTATCGAAGCAGGTAACCAGTTCATCACACACTTTCTGATACTCTTCGCCGCCGt < 1:1751594/71‑1 (MQ=255) aaaGTCAGATCGGGATTATCGAAGCAGGCAACCAGtt < 1:1350372/37‑1 (MQ=255) aaaGTCAGATCGGGATTATCGAAGCAGGCAACCAGTtc < 1:3764365/38‑1 (MQ=255) aaaGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTTTGATACGCTTCGCCGCCGt > 1:3771038/1‑70 (MQ=255) aaaGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATa > 1:4076003/1‑56 (MQ=255) aaaGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGtt < 1:2515395/71‑1 (MQ=255) aGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTc < 1:2138887/49‑1 (MQ=255) gTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAAt < 1:3579454/71‑1 (MQ=255) tCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATa > 1:869917/1‑71 (MQ=255) aGATCGGGATTATCGAAGCAGGTAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATAc > 1:380225/1‑70 (MQ=255) aGATCGGGATTATCGAAGCAGGTAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATAc > 1:3059866/1‑70 (MQ=255) gATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCt < 1:1305139/45‑1 (MQ=255) tCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGa < 1:218267/45‑1 (MQ=255) tCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTaa < 1:1523383/64‑1 (MQ=255) cGGGATTATCGAAGCAGGTAACCAGTTCATCACACACTTTCTGATACGc > 1:2842030/1‑49 (MQ=255) cGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATAc < 1:454538/47‑1 (MQ=255) ttATCGAAGCAGGCAACCAGTTCATCACACACttt < 1:2430396/35‑1 (MQ=255) ttATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGt > 1:2209902/1‑71 (MQ=255) tATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGtt > 1:1518235/1‑71 (MQ=255) tATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGtt > 1:1512807/1‑71 (MQ=255) aTCGAAGCAGGCAACCAGTTCATCACACACTTTCTGa > 1:4230142/1‑37 (MQ=255) aTCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGttt > 1:3847420/1‑71 (MQ=255) tCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTg < 1:3417979/71‑1 (MQ=255) gAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTa > 1:3822886/1‑52 (MQ=255) aaGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTgcgc < 1:817653/71‑1 (MQ=255) aaGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTgcgc < 1:2716216/71‑1 (MQ=255) aaGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTgcgc < 1:3216633/71‑1 (MQ=255) gCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGtt < 1:593844/44‑1 (MQ=255) | ATACCAGTATCAATCATAGACCTTAAGATACGGGCAGAGAAAGTCAGATCGGGATTATCGAAGCAGGCAACCAGTTCATCACACACTTTCTGATACGCTTCGCCGCCGTTAATACTATCCAGCGTTTGCGC > W3110S.gb/2813717‑2813847 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |