| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,818,985 | A→G | 30.0% | R561R (CGT→CGC) | alaS ← | alanyl‑tRNA synthetase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,818,985 | 0 | A | G | 30.0% | 50.4 / 30.4 | 40 | R561R (CGT→CGC) | alaS | alanyl‑tRNA synthetase |
| Reads supporting (aligned to +/- strand): ref base A (13/15); new base G (7/5); total (20/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.31e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.10e-01 | |||||||||||
CGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCGCA > W3110S.gb/2818919‑2819054 | cGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGaa < 1:805349/71‑1 (MQ=255) aTACATGAGTACCCAGAACCTGTCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGGCGAATa < 1:331719/71‑1 (MQ=255) aGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGGGGAGTGATTCAGGCGAATACGGgcgc > 1:4476612/1‑71 (MQ=255) gTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGgcgc > 1:585487/1‑70 (MQ=255) cccAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACg > 1:285144/1‑62 (MQ=255) cccAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGcgac > 1:2494472/1‑70 (MQ=255) ccAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGcgacga < 1:2144154/71‑1 (MQ=255) aGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGGCGAATACGGGCGCGACGAGc > 1:246359/1‑71 (MQ=255) aaCCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGcgacga < 1:3983205/67‑1 (MQ=255) aaCCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGcgacga < 1:2069321/67‑1 (MQ=255) aCCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGcgacga < 1:2069323/66‑1 (MQ=255) aCCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCt > 1:3574699/1‑70 (MQ=255) aCCTGGCGCAGCGAAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCt > 1:3718385/1‑70 (MQ=255) ggCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCtcatc > 1:2419570/1‑70 (MQ=255) gcagcgcagcGTGCATCAGGTGCGTTGCGGAGTGATTCAGGCGaa < 1:4587971/45‑1 (MQ=255) gcagcgcagcGTGCATCAGGTGCGTTGCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCAACa < 1:4263694/71‑1 (MQ=255) gcagcgTGCATCAGGTGCGTTGCGGAGTGATTCAGACGaa > 1:3799821/1‑40 (MQ=255) cagcgTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCt > 1:78570/1‑57 (MQ=255) gcgTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGcgcg < 1:2925573/47‑1 (MQ=255) tCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGcgacga < 1:3317600/44‑1 (MQ=255) gCGTTGCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGcc > 1:2687533/1‑70 (MQ=255) cGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCt < 1:2740858/41‑1 (MQ=255) cGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCa < 1:1294016/71‑1 (MQ=255) ttGCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCaa < 1:1829795/45‑1 (MQ=255) ttGCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCaa < 1:4349939/45‑1 (MQ=255) tGCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCAACATCAGcc > 1:2863259/1‑52 (MQ=255) gCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCAACATCAGCCt > 1:4428995/1‑52 (MQ=255) gCGGAGTGATTCAGGCGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACttt > 1:1161909/1‑71 (MQ=255) gCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACttt > 1:4081193/1‑71 (MQ=255) gCGGAGTGATTCAGACGAATACGGGAGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACttt > 1:1656318/1‑71 (MQ=255) cGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTc < 1:1735841/71‑1 (MQ=255) aGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGcc > 1:1843428/1‑47 (MQ=255) gTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCagag < 1:4137387/71‑1 (MQ=255) tGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAgaga < 1:4128247/71‑1 (MQ=255) aTTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAAc > 1:3910492/1‑71 (MQ=255) ttCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACcgc < 1:16924/50‑1 (MQ=255) cAGGCGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCg > 1:3635389/1‑71 (MQ=255) cAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGcc > 1:2348865/1‑40 (MQ=255) aGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCg < 1:4576075/70‑1 (MQ=255) gACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCagag < 1:2807897/63‑1 (MQ=255) aCGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCgca < 1:476829/70‑1 (MQ=255) | CGATACATGAGTACCCAGAACCTGGCGCAGCGCAGCGTGCATCAGGTGCGTTGCGGAGTGATTCAGACGAATACGGGCGCGACGAGCCTCATCAACATCAGCCTGCACCGCGTCGCCCACTTTCAGAGAACCCGCA > W3110S.gb/2818919‑2819054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |