Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 2,829,103 T→C 7.5% V212A (GTG→GCG)  gutQ → predicted phosphosugar‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,829,1030TC 7.5% 175.8 / 6.1 67V212A (GTG→GCG) gutQpredicted phosphosugar‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (36/26);  new base C (2/3);  total (38/29)
Fisher's exact test for biased strand distribution p-value = 6.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.40e-01

GGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTA  >  W3110S.gb/2829034‑2829173
                                                                     |                                                                      
gggCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTg                                                                       <  1:3266824/71‑1 (MQ=255)
 ggCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTgg                                                                      >  1:2885209/1‑71 (MQ=255)
 ggCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTgg                                                                      <  1:3807638/71‑1 (MQ=255)
 ggCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTg                                                                       >  1:327546/1‑70 (MQ=255)
      tGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGtt                                                                  >  1:833568/1‑70 (MQ=255)
       gggCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTaa                                                                >  1:4297322/1‑71 (MQ=255)
       gggCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTa                                                                 >  1:2424416/1‑70 (MQ=255)
        ggCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTa                                                                 <  1:76346/69‑1 (MQ=255)
         gcgcTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAAcc                                                              >  1:4487908/1‑71 (MQ=255)
           gctcgctTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGc                                                            <  1:2811447/71‑1 (MQ=255)
           gctcgctTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGc                                                            <  1:1082614/71‑1 (MQ=255)
             tcgctTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTa                                                                 <  1:3625197/64‑1 (MQ=255)
             tcgctTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGc                                                            >  1:585329/1‑69 (MQ=255)
              cgctTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAg                                                         >  1:2752376/1‑71 (MQ=255)
               gctTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGc                                                        >  1:3235935/1‑71 (MQ=255)
                   gCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGt                                                      <  1:3854077/69‑1 (MQ=255)
                   gCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGt                                                      <  1:1324851/69‑1 (MQ=255)
                    cTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTgat                                                   <  1:1216089/71‑1 (MQ=255)
                        aTAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGt                                                                   >  1:4008626/1‑51 (MQ=255)
                        aTAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGt                                                                   >  1:3350173/1‑51 (MQ=255)
                         tAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTga                                                    >  1:770605/1‑65 (MQ=255)
                          aaaGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGCGGCGTTAACCGCCAGCGTGATGgatgc                                             >  1:427738/1‑71 (MQ=255)
                            aGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGCGGCGTTAACCGc                                                            >  1:2741373/1‑54 (MQ=255)
                             gTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTgatgga                                                >  1:1976045/1‑65 (MQ=255)
                                catcatCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCt                                       <  1:57854/71‑1 (MQ=255)
                                  tcatcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTa                                                                 <  1:3122352/43‑1 (MQ=255)
                                  tcatcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTg                                      >  1:2344149/1‑70 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCg                                                                    >  1:2747687/1‑38 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAAcc                                                              >  1:394/1‑44 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAAcc                                                              >  1:3951345/1‑44 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAAc                                                               >  1:2136185/1‑43 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGt                                                      <  1:1751122/52‑1 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTgatgg                                                 >  1:2711574/1‑57 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTgatgg                                                 >  1:4192919/1‑57 (MQ=255)
                                    atcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGaa                                   >  1:3632851/1‑71 (MQ=255)
                                     tcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGtt                                                                  <  1:3922208/39‑1 (MQ=255)
                                     tcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGtt                                                                  <  1:2854086/39‑1 (MQ=255)
                                     tcTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAAc                                  >  1:3079609/1‑71 (MQ=255)
                                      cTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTaa                                                                >  1:1859222/1‑40 (MQ=255)
                                       tGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCGGCGTgat                                                   >  1:3652008/1‑52 (MQ=255)
                                       tGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACt                                 >  1:2313813/1‑70 (MQ=255)
                                         aTGCGCCGTTACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGGGATGGATGCGATGCTGGAACTCAg                              <  1:3630970/71‑1 (MQ=255)
                                         aTGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCa                               <  1:3896247/70‑1 (MQ=255)
                                            cgcCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAAc                                  <  1:3098905/64‑1 (MQ=255)
                                            cgcCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAAc                                  <  1:1379180/64‑1 (MQ=255)
                                            cgcCGTGACGATGCCATCCCACAGGCGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGcc                            <  1:352480/70‑1 (MQ=255)
                                                 tGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCg                      <  1:1324297/71‑1 (MQ=255)
                                                   aCGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCgg                     >  1:4561563/1‑70 (MQ=255)
                                                   aCGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGt                    >  1:3833675/1‑71 (MQ=255)
                                                   aCGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGt                    >  1:4499956/1‑71 (MQ=255)
                                                   aCGATGACATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGt                    >  1:8771/1‑71 (MQ=255)
                                                     gATGCCATCCCACAGGCGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCt                  <  1:2522530/71‑1 (MQ=255)
                                                      aTGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTg                 <  1:880982/71‑1 (MQ=255)
                                                       tGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTgg                >  1:963566/1‑71 (MQ=255)
                                                       tGCCATCCCACAGGCGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCt                  <  1:1031417/69‑1 (MQ=255)
                                                           aTCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCt            <  1:3055783/71‑1 (MQ=255)
                                                             cccACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAAc                                  >  1:3860749/1‑47 (MQ=255)
                                                             cccACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCtg           >  1:2954332/1‑70 (MQ=255)
                                                              ccACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGc                             >  1:4389059/1‑51 (MQ=255)
                                                                 caGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGc      >  1:1837444/1‑71 (MQ=255)
                                                                   ggTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGaa                                   >  1:4627831/1‑40 (MQ=255)
                                                                   ggTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTc                   >  1:3584058/1‑56 (MQ=255)
                                                                    gTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCt                  <  1:4450338/56‑1 (MQ=255)
                                                                    gTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCgg    <  1:584460/70‑1 (MQ=255)
                                                                    gTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGt   <  1:3103462/71‑1 (MQ=255)
                                                                    gTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGt   <  1:3572035/71‑1 (MQ=255)
                                                                    gTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGGCt                  <  1:2917068/56‑1 (MQ=255)
                                                                     tGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTa  <  1:2744356/71‑1 (MQ=255)
                                                                     |                                                                      
GGGCACTGGGCGCTCGCTTGCTGAATAAAGTGCATCATCTGATGCGCCGTGACGATGCCATCCCACAGGTGGCGTTAACCGCCAGCGTGATGGATGCGATGCTGGAACTCAGCCGCACCGGTCTGGGGCTGGTGGCGGTA  >  W3110S.gb/2829034‑2829173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: