| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,863,108 | T→C | 13.2% | L73L (TTA→CTA) | ygbM → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,863,108 | 0 | T | C | 13.2% | 128.8 / 12.6 | 53 | L73L (TTA→CTA) | ygbM | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (20/26); new base C (3/4); total (23/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
GAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGG > W3110S.gb/2863039‑2863177 | gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt > 1:3720210/1‑70 (MQ=255)gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt > 1:4334056/1‑70 (MQ=255)gAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt > 1:4602708/1‑70 (MQ=255) cAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt < 1:2615247/67‑1 (MQ=255) aaaCCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCg > 1:525366/1‑71 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGAt > 1:898868/1‑57 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTccc > 1:79603/1‑71 (MQ=255) tGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTccc > 1:2489754/1‑71 (MQ=255) cTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACg < 1:2645697/71‑1 (MQ=255) ggCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGcc > 1:508512/1‑58 (MQ=255) gcgcTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCgg > 1:1456660/1‑65 (MQ=255) gcgcTGTTCAATAACGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGt > 1:2141616/1‑69 (MQ=255) gcTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGc < 1:1474603/71‑1 (MQ=255) gTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATcc > 1:574321/1‑49 (MQ=255) gTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTccc > 1:1332902/1‑58 (MQ=255) ttCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCAc < 1:3936181/69‑1 (MQ=255) ttCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACg < 1:4200199/70‑1 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTAt > 1:2842914/1‑44 (MQ=255) cAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGaa < 1:1073119/70‑1 (MQ=255) aaTACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAg < 1:3052732/70‑1 (MQ=255) tACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTc > 1:671103/1‑50 (MQ=255) cGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:2631205/67‑1 (MQ=255) cGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGca < 1:3724524/67‑1 (MQ=255) tCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:397795/70‑1 (MQ=255) ccGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGAc < 1:2379464/71‑1 (MQ=255) ccGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTT‑‑CGGACGTGAGCACg < 1:1598022/55‑1 (MQ=255) ggAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACAt < 1:4235408/71‑1 (MQ=255) gagaTATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATc < 1:344467/71‑1 (MQ=255) gagaTATTAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATc < 1:1683855/71‑1 (MQ=255) agaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATc > 1:2449771/1‑70 (MQ=255) agaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:3915736/71‑1 (MQ=255) gaTATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGa < 1:662941/71‑1 (MQ=255) tatTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGaa > 1:4127546/1‑52 (MQ=255) atTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCt > 1:3106308/1‑71 (MQ=255) ttAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:2005664/59‑1 (MQ=255) ttAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTg > 1:4193419/1‑71 (MQ=255) ttAATGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCg < 1:2895901/66‑1 (MQ=255) aaTGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGa > 1:1409262/1‑65 (MQ=255) aaTGCGGGGGAATGGGGACTATCCGCCCTTCCCGGACGTGAGCACGAAGca > 1:3445802/1‑51 (MQ=255) aTGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGc < 1:1509555/70‑1 (MQ=255) aTGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGc < 1:100954/70‑1 (MQ=255) aTGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCa < 1:2392377/71‑1 (MQ=255) aTGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCa < 1:4164582/71‑1 (MQ=255) gCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACt > 1:3027187/1‑71 (MQ=255) cGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCa < 1:543946/68‑1 (MQ=255) ggggAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTgg < 1:558594/70‑1 (MQ=255) gggAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGaa < 1:4041143/71‑1 (MQ=255) ggAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:2737609/49‑1 (MQ=255) aTGGGGATTATCCTCCCTTCCCGGACGTGAGCACGAAGCACACGCa < 1:4134870/46‑1 (MQ=255) tGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGc < 1:3358334/38‑1 (MQ=255) ggggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGc < 1:1893420/70‑1 (MQ=255) ggggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCa > 1:1741096/1‑71 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGAc > 1:2550465/1‑52 (MQ=255) gggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCAt > 1:846101/1‑71 (MQ=255) ggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACa > 1:4270148/1‑46 (MQ=255) ggATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACAt > 1:420398/1‑47 (MQ=255) gATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTg < 1:2838344/71‑1 (MQ=255) aTTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTgg > 1:3259327/1‑71 (MQ=255) | GAGCAAAACCATCTGACACTGGCGCTGTTCAATACCGCTCCCGGAGATATTAATGCGGGGGAATGGGGATTATCCGCCCTTCCCGGACGTGAGCACGAAGCACACGCAGACATCGACCTGGCACTGGAATATGCATTGG > W3110S.gb/2863039‑2863177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |