| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,866,110 | T→A | 100% | intergenic (‑20/+160) | rpoS ← / ← nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,866,110 | 0 | T | A | 100.0% | 156.0 / NA | 54 | intergenic (‑20/+160) | rpoS/nlpD | RNA polymerase, sigma S (sigma 38) factor/predicted outer membrane lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (28/26); total (28/26) | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTA > W3110S.gb/2866046‑2866176 | cacacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta < 1:4386990/71‑1 (MQ=255)cacacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:3727517/1‑71 (MQ=255) acacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctac > 1:3150776/1‑71 (MQ=255) cacGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:148773/71‑1 (MQ=255) tgtgGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:1374172/60‑1 (MQ=255) gCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCtt < 1:2629769/68‑1 (MQ=255) gCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTc < 1:3219777/71‑1 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctacta < 1:3351216/55‑1 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:4218476/54‑1 (MQ=255) tGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa > 1:1185318/1‑70 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTc > 1:3468156/1‑49 (MQ=255) gCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaa > 1:3253846/1‑70 (MQ=255) gCGATAACAGTTCCTCGTCGGCCAAATCGTTATCACTAGGTTCCAATTc > 1:4034559/1‑49 (MQ=255) aTAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAAcc > 1:2089359/1‑71 (MQ=255) tAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTctact < 1:2917717/49‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:3390754/70‑1 (MQ=255) aCAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTc < 1:3394268/70‑1 (MQ=255) gTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTc < 1:3976268/58‑1 (MQ=255) ttCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAg < 1:2882982/46‑1 (MQ=255) ttCCTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCa < 1:1735439/58‑1 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTTCTAAGGCCTTTTCGTCAAAAACCTCaa > 1:328929/1‑66 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTaa > 1:1191654/1‑43 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTc > 1:588506/1‑52 (MQ=255) ccTCTTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTc > 1:2550076/1‑52 (MQ=255) ctctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCg < 1:4335350/70‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:3818000/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTc < 1:1295438/35‑1 (MQ=255) tctTCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGt > 1:2661158/1‑70 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:4355800/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:1063110/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:3265572/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:1397964/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:1988448/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:2097882/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTcta > 1:2300062/1‑35 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaaaaa > 1:521559/1‑56 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGtt < 1:2212885/69‑1 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct > 1:739225/1‑71 (MQ=255) ttCGGCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct > 1:762110/1‑71 (MQ=255) ggCCAAATCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCtca < 1:79084/70‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCaa < 1:3737492/46‑1 (MQ=255) aaaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCaaa < 1:514463/71‑1 (MQ=255) aaTCGTTATCACTGGGTTCCAATTCTACTAAGGCCttt > 1:2201992/1‑38 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCtttt > 1:4577305/1‑37 (MQ=255) tCGTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTc > 1:3333592/1‑41 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCg < 1:2699986/71‑1 (MQ=255) gTTATCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAAATCCg < 1:603880/71‑1 (MQ=255) aTCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCa < 1:2381811/70‑1 (MQ=255) tCACTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATc < 1:1318436/71‑1 (MQ=255) aCTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct > 1:990097/1‑53 (MQ=255) aCTGGGTTCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCtt < 1:999380/71‑1 (MQ=255) aCTGGGTTCAAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTtct > 1:3849279/1‑53 (MQ=255) gggTTCCAATTCTACTAAGTCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCAt > 1:2759216/1‑71 (MQ=255) ttCCAATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTa < 1:1184674/71‑1 (MQ=255) | CACACGCTGTGTGGCTCCCTGCGATAACAGTTCCTCTTCGGCCAAATCGTTATCACTGGGTTCCTATTCTACTAAGGCCTTTTCGTCAAAAACCTCAACTCCGTTCTCATCAAATTCCGCATCTTCATTTA > W3110S.gb/2866046‑2866176 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |