| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,886,205 | A→G | 17.4% | intergenic (‑330/+29) | ygcB ← / ← cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,886,205 | 0 | A | G | 17.4% | 45.9 / 5.4 | 23 | intergenic (‑330/+29) | ygcB/cysH | conserved hypothetical protein, member of DEAD box family/3'‑phosphoadenosine 5'‑phosphosulfate reductase |
| Reads supporting (aligned to +/- strand): ref base A (14/5); new base G (2/2); total (16/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
CTTCCCGCGGCGTTCACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGA > W3110S.gb/2886136‑2886274 | cttcCCGCGGCGTTCACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGa > 1:454966/1‑70 (MQ=255) cGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTg > 1:1867762/1‑71 (MQ=255) tttAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTc > 1:21804/1‑71 (MQ=255) ttAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTcc > 1:496601/1‑71 (MQ=255) aTCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGGCAGAGGCGTTTAATTTGTCCGGCaa < 1:3842202/71‑1 (MQ=255) gAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGa > 1:4014199/1‑41 (MQ=255) ctgctCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCt > 1:196715/1‑71 (MQ=255) tCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTc < 1:4542581/69‑1 (MQ=255) aaaaTAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAat < 1:2472644/54‑1 (MQ=255) aTAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCa > 1:1469177/1‑49 (MQ=255) gCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAat < 1:2467253/43‑1 (MQ=255) cccATCATTTCTAACAGAGGCGTTTAATTTGTCCGGCAATAttt < 1:3944949/44‑1 (MQ=255) catcatTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTAc > 1:717058/1‑44 (MQ=255) tcatTTCTGACAGAGGCGTTTAATTTGTCCGGCAATAtt < 1:3820293/39‑1 (MQ=255) atTTCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGc > 1:1076977/1‑71 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTAc > 1:4511878/1‑40 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCgtgt > 1:4008992/1‑49 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACAtt > 1:1036508/1‑59 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGc > 1:3913269/1‑71 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGc > 1:4520072/1‑71 (MQ=255) atTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGc > 1:4605929/1‑71 (MQ=255) tttCTGGCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGcc < 1:1583728/71‑1 (MQ=255) tCTGACAGAGGCGTTTAATTTGTCCTGCAATATTTACCCTTCgt > 1:2211520/1‑44 (MQ=255) tCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCgt > 1:4478861/1‑44 (MQ=255) cTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCa > 1:1317160/1‑69 (MQ=255) aCAGAGGCGTTTAATTTGTCCGGCGATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGa < 1:1536127/70‑1 (MQ=255) aCAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGa < 1:3685983/70‑1 (MQ=255) | CTTCCCGCGGCGTTCACGTTTAAAATCAGGAATGCTGCTCGCAAAATAAAAACGCCCCCATCATTTCTGACAGAGGCGTTTAATTTGTCCGGCAATATTTACCCTTCGTGTAACCCACATTCCCTTTTTAAGCCAAAGA > W3110S.gb/2886136‑2886274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |