| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,892,483 | A→G | 17.7% | K391E (AAA→GAA) | ygcN → | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,892,483 | 0 | A | G | 17.7% | 141.3 / 24.9 | 62 | K391E (AAA→GAA) | ygcN | predicted oxidoreductase, FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base A (29/22); new base G (4/7); total (33/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGG > W3110S.gb/2892419‑2892553 | aTGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTg < 1:1054594/71‑1 (MQ=255) tGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGt > 1:1811645/1‑71 (MQ=255) gTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTc < 1:206307/71‑1 (MQ=255) tACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATaa < 1:3222729/62‑1 (MQ=255) tACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTcc < 1:1733329/71‑1 (MQ=255) aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCa > 1:898134/1‑71 (MQ=255) aCCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCa > 1:4569289/1‑71 (MQ=255) ccGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGt < 1:1635256/66‑1 (MQ=255) ccGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCa < 1:572722/70‑1 (MQ=255) ccGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc > 1:1244204/1‑71 (MQ=255) cGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCt > 1:3811865/1‑70 (MQ=255) cGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTg > 1:767824/1‑71 (MQ=255) cGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTg > 1:404370/1‑71 (MQ=255) gCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGcc < 1:1482374/71‑1 (MQ=255) cTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAg > 1:1586026/1‑71 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACATGTTCCACCGCTGCGCCAGTTa < 1:4405630/71‑1 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGt < 1:1831731/69‑1 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGt < 1:1158901/69‑1 (MQ=255) cGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTa < 1:3548142/71‑1 (MQ=255) gTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTc > 1:832457/1‑53 (MQ=255) aaTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCt > 1:365571/1‑71 (MQ=255) aaTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCa > 1:1106177/1‑52 (MQ=255) aaTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTc > 1:3791895/1‑70 (MQ=255) aTGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTg > 1:4080515/1‑71 (MQ=255) tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAAc < 1:2516791/42‑1 (MQ=255) tGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTg < 1:2633603/45‑1 (MQ=255) cAGGATATTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTc > 1:3571077/1‑66 (MQ=255) cAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAAc > 1:3597742/1‑40 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTgg < 1:2126331/66‑1 (MQ=255) gATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatca > 1:2866988/1‑71 (MQ=255) atatTTCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatcat < 1:2044963/71‑1 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTcc > 1:2729720/1‑42 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatcat < 1:3119019/70‑1 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt < 1:1748417/71‑1 (MQ=255) tatTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt > 1:4515121/1‑71 (MQ=255) tttCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCTCCAGTTATTCTGGca < 1:2336657/64‑1 (MQ=255) ttCCCGCGATTTATGTGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTgg < 1:1533881/61‑1 (MQ=255) tCCCGCGATTTATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAc < 1:1127636/70‑1 (MQ=255) cccGCGATTTATGGGATCAGGGTGATAAACCTGTTCCa > 1:739866/1‑38 (MQ=255) cccGCGATTTATGGGATCAGGGTGATAAACCTGTTCCAc > 1:745082/1‑39 (MQ=255) gcgATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCt > 1:2341398/1‑54 (MQ=255) gATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt < 1:2792409/61‑1 (MQ=255) tATGGGATCAGGGTGATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAttt < 1:2816457/58‑1 (MQ=255) tGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCa > 1:752620/1‑65 (MQ=255) ggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgt < 1:3839318/71‑1 (MQ=255) ggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgt < 1:2046457/71‑1 (MQ=255) ggATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtg < 1:4645410/70‑1 (MQ=255) aTCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatcat > 1:1069095/1‑50 (MQ=255) aTCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAtt < 1:2335458/51‑1 (MQ=255) tCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGc < 1:2264449/71‑1 (MQ=255) cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTAtt > 1:1208187/1‑38 (MQ=255) cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTAtt > 1:4555071/1‑38 (MQ=255) cAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTg > 1:4462774/1‑41 (MQ=255) aGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCa > 1:3360403/1‑70 (MQ=255) aGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCAt > 1:2864845/1‑71 (MQ=255) gTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTg > 1:3051578/1‑71 (MQ=255) gTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGACTGTGGCATCTg > 1:4417646/1‑71 (MQ=255) gATGAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCAttt < 1:1317922/44‑1 (MQ=255) gATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTggc > 1:814758/1‑71 (MQ=255) aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGcatca < 1:749461/40‑1 (MQ=255) aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTAcg > 1:2304883/1‑46 (MQ=255) aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgtg > 1:3879548/1‑61 (MQ=255) aTAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCtgt < 1:3129487/60‑1 (MQ=255) aaaCCTGTTCCACCGCTGCGCCAGTTATTCTGGcatc > 1:1080794/1‑37 (MQ=255) aaaCCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTggcggg > 1:500490/1‑71 (MQ=255) aaaCCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTggcggg > 1:4454749/1‑71 (MQ=255) | ATGGTACCGTACGTGGCCTGCGTTAATGCAGGATATTTCCCGCGATTTATGGGATCAGGGTGATAAACCTGTTCCACCGCTGCGCCAGTTATTCTGGCATCATTTACGTCGTCACGGCCTGTGGCATCTGGCGGG > W3110S.gb/2892419‑2892553 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |