| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,902,330 | A→T | 36.4% | intergenic (+300/+1073) | ygcE → / ← ygcF | predicted kinase/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,902,330 | 0 | A | T | 36.4% | 51.1 / 57.2 | 55 | intergenic (+300/+1073) | ygcE/ygcF | predicted kinase/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (18/17); new base T (10/10); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
AATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATT > W3110S.gb/2902267‑2902390 | aaTTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCgg > 1:1530304/1‑71 (MQ=255) ttttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGt > 1:1352776/1‑70 (MQ=255) ttttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGt > 1:3088489/1‑70 (MQ=255) ttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCgg > 1:4432188/1‑67 (MQ=255) ttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTAt > 1:1402591/1‑71 (MQ=255) cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATaga > 1:1960486/1‑60 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGt < 1:2669737/64‑1 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaa < 1:3578172/71‑1 (MQ=255) cTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaa < 1:2859153/71‑1 (MQ=255) aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAAc > 1:1786813/1‑71 (MQ=255) aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTaa < 1:3727513/68‑1 (MQ=255) aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAc > 1:895482/1‑71 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata > 1:501137/1‑47 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata > 1:698911/1‑47 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGtata < 1:269141/47‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTc < 1:2234410/70‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCa < 1:21216/71‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCa < 1:1302075/71‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCa < 1:2762660/71‑1 (MQ=255) tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACACCGtata < 1:601445/47‑1 (MQ=255) cTGGATATCTTTATTAATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTaa < 1:2146992/58‑1 (MQ=255) gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATaga > 1:3344365/1‑42 (MQ=255) gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGc < 1:1482786/69‑1 (MQ=255) gATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCt > 1:2886008/1‑71 (MQ=255) tatCTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTaa < 1:1387081/53‑1 (MQ=255) atCTTTATTCATAAGAACTATTCATCAACAGCGTATtga > 1:3718111/1‑39 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa < 1:93296/70‑1 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa < 1:954002/70‑1 (MQ=255) tCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa > 1:32129/1‑70 (MQ=255) cTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaaa > 1:4518697/1‑71 (MQ=255) cTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaaa > 1:2532206/1‑71 (MQ=255) cTTTATTCATAAGAACTATTCATCAACAGCGTATTGAGGAGGTTATGTAAAACCACTCATTAGCCTCaaaa > 1:3032692/1‑71 (MQ=255) cTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaaaa > 1:2764734/1‑71 (MQ=255) ttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTa > 1:3523175/1‑43 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCAt > 1:638494/1‑57 (MQ=255) tATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa < 1:3146199/66‑1 (MQ=255) ttCATAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCAtt > 1:1575210/1‑56 (MQ=255) cATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTa > 1:1253695/1‑42 (MQ=255) aTAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACa < 1:4314271/71‑1 (MQ=255) tAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTaaa > 1:1732440/1‑42 (MQ=255) tAAGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACa < 1:2991657/70‑1 (MQ=255) aGAACTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAAc < 1:2508392/71‑1 (MQ=255) aGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaaa < 1:631564/70‑1 (MQ=255) aCTATTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTc < 1:2875807/71‑1 (MQ=255) aTTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaaa > 1:3480796/1‑64 (MQ=255) aTTCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTc > 1:4328188/1‑68 (MQ=255) ttCATCAACAGCGTATTGAGGCGGTTATGTAAAACCACTCa < 1:4219699/41‑1 (MQ=255) ttCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTc > 1:155281/1‑67 (MQ=255) tcatcaACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACaaa < 1:1622163/56‑1 (MQ=255) tcatcaACAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTcc > 1:4302472/1‑67 (MQ=255) tcatcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAt > 1:2085051/1‑71 (MQ=255) catcaACAGCGTATATAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAtt > 1:3998782/1‑71 (MQ=255) atcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGAttt < 1:1174674/71‑1 (MQ=255) tcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCaa < 1:1952979/47‑1 (MQ=255) tcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGa < 1:3057458/55‑1 (MQ=255) tcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACaa < 1:537629/58‑1 (MQ=255) aaCAGCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttat > 1:3857647/1‑70 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttatt < 1:4232928/71‑1 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttat > 1:4534203/1‑70 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCAAGAt > 1:3852384/1‑66 (MQ=255) aaCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCAAGATttat > 1:4396570/1‑70 (MQ=255) gCGTATTGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATttat < 1:1090097/66‑1 (MQ=255) | AATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTAGCCTCAAAACAAAGACAAACTTCCAGATTTATT > W3110S.gb/2902267‑2902390 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |