| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,960,805 | T→C | 27.3% | K94E (AAA→GAA) | recC ← | exonuclease V (RecBCD complex), gamma chain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,960,805 | 0 | T | C | 27.3% | 84.8 / 37.9 | 55 | K94E (AAA→GAA) | recC | exonuclease V (RecBCD complex), gamma chain |
| Reads supporting (aligned to +/- strand): ref base T (18/22); new base C (8/7); total (26/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCGGACGAACATATC > W3110S.gb/2960741‑2960874 | agATAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCt > 1:1102879/1‑71 (MQ=255)agATAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCt < 1:2907066/71‑1 (MQ=255)agATAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCt < 1:216056/71‑1 (MQ=255) aTAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCa > 1:2291301/1‑71 (MQ=255) tAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCAt > 1:1621556/1‑71 (MQ=255) aTGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATg < 1:983534/70‑1 (MQ=255) aTGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATg < 1:4222699/70‑1 (MQ=255) aTGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATg < 1:3731430/70‑1 (MQ=255) aTGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATg < 1:3044466/70‑1 (MQ=255) aTGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGc > 1:848954/1‑71 (MQ=255) aTGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGc > 1:1123110/1‑71 (MQ=255) tGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGct > 1:2504413/1‑71 (MQ=255) cGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCtg < 1:497561/71‑1 (MQ=255) cGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCtg < 1:2350377/71‑1 (MQ=255) aaCAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCt < 1:2838695/59‑1 (MQ=255) aCAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCtgtttg < 1:283698/71‑1 (MQ=255) aCAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCtgtt < 1:3230851/69‑1 (MQ=255) gggTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTa < 1:3527651/71‑1 (MQ=255) ggTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGct < 1:2000782/60‑1 (MQ=255) tAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAg > 1:1472283/1‑71 (MQ=255) tAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAg < 1:2071806/71‑1 (MQ=255) aaGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGgc > 1:1422639/1‑71 (MQ=255) gTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTcc < 1:647683/44‑1 (MQ=255) tCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCtgtttgtt < 1:3720998/62‑1 (MQ=255) cTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAg > 1:3931594/1‑44 (MQ=255) ttCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGctct > 1:3202061/1‑71 (MQ=255) ttCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGct > 1:2535083/1‑69 (MQ=255) ttCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTAAAGGCGctct > 1:182061/1‑71 (MQ=255) gcgcTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCtt < 1:2327206/71‑1 (MQ=255) cTCCAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTgg > 1:253633/1‑71 (MQ=255) cAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTgggg < 1:3731541/70‑1 (MQ=255) cAGCAATTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGa > 1:1362950/1‑71 (MQ=255) aGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCtt < 1:2613722/64‑1 (MQ=255) gCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTc < 1:2630177/35‑1 (MQ=255) gCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCa > 1:1639464/1‑36 (MQ=255) gCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTaa > 1:3672798/1‑51 (MQ=255) aaTTGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTc > 1:1097933/1‑71 (MQ=255) ttGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTa < 1:4177685/46‑1 (MQ=255) ttGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCtt < 1:2535469/59‑1 (MQ=255) ttGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTcc < 1:2032855/70‑1 (MQ=255) ttGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTcc < 1:4093739/70‑1 (MQ=255) ttGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCg < 1:4322629/71‑1 (MQ=255) tGCGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGAt > 1:2781745/1‑65 (MQ=255) gCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCgg > 1:2342219/1‑70 (MQ=255) gCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCgg > 1:1341533/1‑70 (MQ=255) gCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCgg > 1:2678727/1‑70 (MQ=255) gCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTATGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCgg > 1:1358633/1‑70 (MQ=255) cGGCAGCAGAGTCATCAGTTCCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGt < 1:3848397/70‑1 (MQ=255) ggCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTaa < 1:4268373/71‑1 (MQ=255) agagTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCg < 1:4413587/71‑1 (MQ=255) agagTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCg > 1:1635231/1‑71 (MQ=255) tcatcaGTTTCCAGCTCATGCTCTGTTTGTTAAAgg > 1:2382233/1‑36 (MQ=255) atcaGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCGGACGaa > 1:3277495/1‑71 (MQ=255) atcaGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCGGACGaa < 1:2059709/71‑1 (MQ=255) atcaGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCGGACGaa > 1:1560633/1‑71 (MQ=255) tttCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCg < 1:2114868/60‑1 (MQ=255) ttCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCGGACGAACATATc > 1:130544/1‑71 (MQ=255) | AGATAATGCCGCAACAGGGTAAAGTCTTCGCGCTCCAGCAATTGCGGCAGCAGAGTCATCAGTTTCCAGCTCATGCTCTGTTTGTTAAAGGCGCTCTCTTTGGGGATTTCCGGTAACACCCGGACGAACATATC > W3110S.gb/2960741‑2960874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |