| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,966,285 | T→C | 21.2% | E269G (GAG→GGG) | ptsP ← | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,966,285 | 0 | T | C | 21.2% | 63.2 / 14.4 | 33 | E269G (GAG→GGG) | ptsP | fused PEP‑protein phosphotransferase (enzyme I) of PTS system |
| Reads supporting (aligned to +/- strand): ref base T (11/15); new base C (3/4); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAG > W3110S.gb/2966217‑2966346 | tGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCActct > 1:2114498/1‑71 (MQ=255)tGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAct < 1:4470276/69‑1 (MQ=255) tAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCAc > 1:4491620/1‑71 (MQ=255) aGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCAcc > 1:4015731/1‑71 (MQ=255) gTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCAct > 1:868419/1‑62 (MQ=255) gTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCAcc < 1:4002295/70‑1 (MQ=255) tATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGc < 1:4581940/64‑1 (MQ=255) aTCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAg > 1:2119916/1‑70 (MQ=255) aTCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGc > 1:1449984/1‑71 (MQ=255) tCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCActc > 1:301444/1‑59 (MQ=255) aCTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCtt < 1:1264046/71‑1 (MQ=255) aCTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCtt > 1:3595462/1‑71 (MQ=255) tAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCACCGAGCCTTTAt > 1:346319/1‑71 (MQ=255) aGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTatat > 1:2469741/1‑70 (MQ=255) gcgcGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATc < 1:3015051/70‑1 (MQ=255) tCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACaa > 1:819216/1‑71 (MQ=255) cGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAAt > 1:1853701/1‑71 (MQ=255) ggCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAAtt < 1:4051661/71‑1 (MQ=255) gCAAATTTTTCAATGACCGTTTTTACCGCCCACCCTGCCACCGAGCCTTTATc < 1:4594297/53‑1 (MQ=255) gCAAATTTTTCAATGACAGTTTTTACCGCCCACCCTGCCACCGAGCCTTTATCAACCTCGGCAAACAAtt < 1:2819224/70‑1 (MQ=255) cAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCtt < 1:222976/48‑1 (MQ=255) aaaTTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTcgc < 1:2873604/71‑1 (MQ=255) aaaTTTTTCAATGACCGCTTTTACCGCCCACCCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTcgc < 1:2178333/71‑1 (MQ=255) aTTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTAt < 1:3676792/48‑1 (MQ=255) tttttCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGAc < 1:76397/71‑1 (MQ=255) tttttCAATGACCGTTTTTACCGCCCACCCTGCCACCGAGCCTTTATCaa > 1:4328975/1‑50 (MQ=255) tttttCAATGACCGTTTTTACCGCCCACCCTGCCACCGAGCCTTTATCa < 1:2824688/49‑1 (MQ=255) ttttCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTa > 1:1420772/1‑45 (MQ=255) aaTGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGcc < 1:4412355/71‑1 (MQ=255) aTGACCGTTTTTACCGCCCACTCTGCCACCGAGCCtt < 1:3331263/37‑1 (MQ=255) tGACCGTTTTTACCGCCCACTCTGCCACCGAGCCtt < 1:2463189/36‑1 (MQ=255) tGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATc < 1:517060/40‑1 (MQ=255) aCCGTTTTTACCGCCCACCCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGc > 1:118011/1‑66 (MQ=255) cGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATc > 1:426775/1‑36 (MQ=255) cGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCaa > 1:2072823/1‑38 (MQ=255) tttttACCGCCCACTCTGCCACCGAGCCTTTATCAACCt < 1:2947527/39‑1 (MQ=255) tACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGaa < 1:1185424/70‑1 (MQ=255) ctctGCCACCGAGCCTTTATCAACCTCGGCAAACAATTcgc < 1:1012789/41‑1 (MQ=255) ctctGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAg < 1:2543656/63‑1 (MQ=255) | TGAGATAGTTATCACTTAGCGCGGCAAACTGTTCGGCAAATTTTTCAATGACCGTTTTTACCGCCCACTCTGCCACCGAGCCTTTATCAACCTCGGCAAACAATTCGCGACGCAGCCGGGTGTCCGAAAG > W3110S.gb/2966217‑2966346 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |