| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,983,818 | A→G | 17.3% | V144A (GTG→GCG) | yqeF ← | predicted acyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,983,818 | 0 | A | G | 17.3% | 241.7 / 43.2 | 104 | V144A (GTG→GCG) | yqeF | predicted acyltransferase |
| Reads supporting (aligned to +/- strand): ref base A (53/33); new base G (11/7); total (64/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.04e-01 | |||||||||||
Only 100 of 111 total aligned reads displayed.
TCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGC > W3110S.gb/2983749‑2983888 | gcgAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa < 1:242674/71‑1 (MQ=255) cgAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAg < 1:2101353/71‑1 (MQ=255) cAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa > 1:2051603/1‑65 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGt > 1:3640228/1‑70 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGt > 1:3652152/1‑70 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:3158304/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:668137/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:1216594/71‑1 (MQ=255) aGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:1211314/71‑1 (MQ=255) aTTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaa > 1:1606698/1‑71 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaac > 1:2649923/1‑70 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc > 1:1960068/1‑67 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc > 1:3371030/1‑67 (MQ=255) tttCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc > 1:4217504/1‑67 (MQ=255) tCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGa < 1:2364988/60‑1 (MQ=255) cggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaaccaac > 1:3477293/1‑71 (MQ=255) ggcggTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGcaca > 1:917461/1‑55 (MQ=255) gTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGc < 1:171545/70‑1 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa > 1:1530368/1‑50 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:4572231/1‑71 (MQ=255) gACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:1430659/1‑71 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa < 1:4588041/49‑1 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAtt > 1:1846723/1‑71 (MQ=255) acacCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAtt > 1:2919571/1‑71 (MQ=255) acCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGAGCAAGa > 1:1095778/1‑49 (MQ=255) ccAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacca < 1:1432582/58‑1 (MQ=255) aaTATGATAATCATTGAAGGCATCCCACAACCCATCATGCACaa > 1:3417690/1‑44 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTc < 1:3680570/49‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc < 1:2134161/49‑1 (MQ=255) tatGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTa < 1:2359410/62‑1 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGca > 1:1091949/1‑37 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGca > 1:2339131/1‑37 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTc > 1:3539268/1‑47 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtg > 1:4207138/1‑71 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtg < 1:3735881/71‑1 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCt < 1:2201151/70‑1 (MQ=255) tGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCt < 1:452520/70‑1 (MQ=255) tAATCATTGAAGGCATCCAACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:2329266/1‑58 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTcaacc < 1:2812903/45‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGcc < 1:3627148/59‑1 (MQ=255) tCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAcc < 1:1519548/71‑1 (MQ=255) cATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTa > 1:2988129/1‑53 (MQ=255) cATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTa > 1:249996/1‑53 (MQ=255) aTTGAAGGCATCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCgg > 1:161028/1‑71 (MQ=255) ttGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGt > 1:344316/1‑71 (MQ=255) ggCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGc > 1:2221122/1‑71 (MQ=255) aTCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAt < 1:4493252/71‑1 (MQ=255) tCCCACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc < 1:122465/71‑1 (MQ=255) tCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGc < 1:2429197/46‑1 (MQ=255) cccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAt > 1:118005/1‑69 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTa > 1:2272558/1‑40 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGcc > 1:2741674/1‑45 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGcc > 1:2169480/1‑45 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca < 1:1571562/70‑1 (MQ=255) ccACAACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca < 1:3874910/70‑1 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTa > 1:3095586/1‑40 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:1718379/1‑41 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTAt > 1:2221035/1‑41 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:3379077/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:3437016/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:4336855/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcag > 1:2691329/1‑71 (MQ=255) ccACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCAAGGCTGTGCACCGGTGCGGCTAtcag > 1:4280192/1‑71 (MQ=255) ccACAACCCATCATGCACAACACTGTCAACCAACTGGCTATTGCCAAGCTGTGc > 1:77691/1‑54 (MQ=255) ccACAACACATCATGCGCAAGACTGTCAACCAACTGGc > 1:876685/1‑38 (MQ=38) acacaACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGcc > 1:4309935/2‑45 (MQ=255) acaACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtc < 1:3392266/71‑1 (MQ=255) caACCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCa < 1:4007133/43‑1 (MQ=255) aaCCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTg > 1:3277626/1‑57 (MQ=255) aaCCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagt > 1:38640/1‑68 (MQ=255) aCCCATCATGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag > 1:3547547/1‑70 (MQ=255) aCCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtcagtcag > 1:12271/1‑70 (MQ=255) aCCCATCATCCACAACACTGTCAACCAACTCGCTATTGCCAAGCtgt > 1:1251127/1‑47 (MQ=255) ccATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGaa > 1:3615912/1‑70 (MQ=255) ccATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAAc < 1:1861271/71‑1 (MQ=255) catcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCtg < 1:3431128/43‑1 (MQ=255) catcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAc > 1:1504793/1‑48 (MQ=255) catcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAc > 1:1864109/1‑48 (MQ=255) catcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAc > 1:4371319/1‑48 (MQ=255) catcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCAc > 1:1900519/1‑48 (MQ=255) atcatGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAt > 1:1729770/1‑71 (MQ=255) tcatGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCa > 1:1125548/1‑45 (MQ=255) tcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCt < 1:368229/40‑1 (MQ=255) tcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtg < 1:2819595/71‑1 (MQ=255) tcatGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAt < 1:3983482/70‑1 (MQ=255) atGCGCAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:3694270/1‑71 (MQ=255) atGCACAAGACTGTCGACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCgg > 1:2607907/1‑52 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGc > 1:904285/1‑37 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGc > 1:1854217/1‑50 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCg > 1:2470942/1‑51 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtca > 1:1749151/1‑58 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAAc > 1:4060992/1‑66 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtgtg > 1:864119/1‑71 (MQ=255) atGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACAtg < 1:38802/69‑1 (MQ=255) atGCACAAGACTGTCAACCAAATGGCTATTGCCAAGCTGTGCACCGGGGCGGCTATCAGTCAGAACAtg < 1:1401986/69‑1 (MQ=255) atGCACAAGAATGTCAACCAACTGGATATTGCCAAga > 1:4310900/1‑36 (MQ=37) gCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTAtc < 1:113661/55‑1 (MQ=255) cacaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCgg > 1:921025/1‑44 (MQ=255) cacaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATgtggtg > 1:869296/1‑71 (MQ=255) acaAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTgc > 1:873712/1‑71 (MQ=255) | TCGCGAGCCAGATTTTCGGCGGTGACACCAATATGATAATCATTGAAGGCATCCCACAACCCATCATGCACAAGACTGTCAACCAACTGGCTATTGCCAAGCTGTGCACCGGTGCGGCTATCAGTCAGAACATGTGGTGC > W3110S.gb/2983749‑2983888 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |