| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 192,898 | T→C | 25.9% | A9A (GCT→GCC) | frr → | ribosome recycling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 192,898 | 0 | T | C | 25.9% | 88.7 / 34.1 | 54 | A9A (GCT→GCC) | frr | ribosome recycling factor |
| Reads supporting (aligned to +/- strand): ref base T (19/21); new base C (4/10); total (23/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.47e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01 | |||||||||||
GACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTG > W3110S.gb/192829‑192965 | gACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCt > 1:2491511/1‑70 (MQ=255) aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg > 1:971488/1‑70 (MQ=255) aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg < 1:83701/70‑1 (MQ=255) aCTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTg < 1:1198933/70‑1 (MQ=255) gtctggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAg < 1:3541528/70‑1 (MQ=255) tctggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAg < 1:526258/69‑1 (MQ=255) tggtctgACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACg > 1:141028/1‑71 (MQ=255) gtctgACTGAGACAAGTTTTCAAGGATACGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACg < 1:3410076/69‑1 (MQ=255) tgactgaGACAAGTTTTCAATGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAg < 1:1779154/62‑1 (MQ=255) tgactgaGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAg > 1:3644489/1‑62 (MQ=255) tgactgaGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACg < 1:4414413/66‑1 (MQ=255) tgactgaAACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATg > 1:1832831/1‑70 (MQ=255) ctgaGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATg < 1:2146117/67‑1 (MQ=255) agaCAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAAt > 1:2752484/1‑71 (MQ=255) aCAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACAAATGc < 1:1476151/71‑1 (MQ=255) cAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCg < 1:2010665/71‑1 (MQ=255) cAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCg < 1:382479/71‑1 (MQ=255) aaGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGa < 1:4080533/50‑1 (MQ=255) aaGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACg < 1:3807868/56‑1 (MQ=255) aaGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAg > 1:1062750/1‑52 (MQ=255) aaGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAg > 1:894089/1‑52 (MQ=255) aGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATgag > 1:1769555/1‑67 (MQ=255) aGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTa < 1:2552439/71‑1 (MQ=255) ttttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAg > 1:101443/1‑70 (MQ=255) ttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAg > 1:4170230/1‑47 (MQ=255) ttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGaa > 1:1750202/1‑70 (MQ=255) ttCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAg > 1:2328034/1‑71 (MQ=255) gATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACa < 1:648651/53‑1 (MQ=255) gATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCa < 1:3309649/71‑1 (MQ=255) aTTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTa < 1:889526/60‑1 (MQ=255) ttCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCaa > 1:4163289/1‑70 (MQ=255) cGTAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCaaaa < 1:1629616/70‑1 (MQ=255) tAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACa < 1:3331243/47‑1 (MQ=255) tAACGTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACa < 1:4159213/47‑1 (MQ=255) gTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATg > 1:557803/1‑47 (MQ=255) gTGATTAGCGATATCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAAt < 1:3293195/71‑1 (MQ=255) tGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATc < 1:2744160/71‑1 (MQ=255) ttAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAAt < 1:110168/67‑1 (MQ=255) tAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCaaa > 1:686269/1‑65 (MQ=255) tAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAg > 1:1962367/1‑69 (MQ=255) aGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCaa > 1:3491868/1‑56 (MQ=255) cGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaaaa < 1:3434962/71‑1 (MQ=255) cGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCaaaa < 1:4193651/71‑1 (MQ=255) atCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACg > 1:2368869/1‑71 (MQ=255) tCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTa < 1:3449361/38‑1 (MQ=255) tCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACg > 1:3657474/1‑70 (MQ=255) tCAGAAAAGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACg < 1:3509761/70‑1 (MQ=255) cAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTa > 1:3576827/1‑37 (MQ=255) cAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATc < 1:2246943/58‑1 (MQ=255) aGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAAt > 1:1662604/1‑56 (MQ=255) aaGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAg > 1:4394623/1‑55 (MQ=255) aaGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGt > 1:886711/1‑71 (MQ=255) aaGATGCCGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGt < 1:2471707/71‑1 (MQ=255) aTGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGt < 1:316217/71‑1 (MQ=255) gCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTg < 1:4388973/70‑1 (MQ=255) | GACTGTCTGGTCTGACTGAGACAAGTTTTCAAGGATTCGTAACGTGATTAGCGATATCAGAAAAGATGCTGAAGTACGCATGGACAAATGCGTAGAAGCGTTCAAAACCCAAATCAGCAAAATACGCACGGGTCGTG > W3110S.gb/192829‑192965 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |