| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 2,989,906 | A→G | 21.1% | intergenic (+250/‑18) | ygeF → / → ygeG | hypothetical protein/predicted chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,989,906 | 0 | A | G | 21.1% | 139.0 / 36.5 | 71 | intergenic (+250/‑18) | ygeF/ygeG | hypothetical protein/predicted chaperone |
| Reads supporting (aligned to +/- strand): ref base A (22/34); new base G (6/9); total (28/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.91e-01 | |||||||||||
ATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAA > W3110S.gb/2989839‑2989973 | aTAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGacac < 1:3916941/69‑1 (MQ=255) tAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACAt < 1:3363979/70‑1 (MQ=255) tttAAAAATGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATcaca < 1:894740/71‑1 (MQ=255) aaaGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGaa < 1:2511448/71‑1 (MQ=255) aTAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGaa < 1:3204180/67‑1 (MQ=255) aaaaTATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGcaca < 1:4303496/71‑1 (MQ=255) atatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGa > 1:1114230/1‑70 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGaaa < 1:271432/71‑1 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGaaa > 1:1351914/1‑71 (MQ=255) tatCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGaaa > 1:4226260/1‑71 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACa < 1:685771/71‑1 (MQ=255) tCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACa < 1:3693080/71‑1 (MQ=255) cAATGAGTCAATCAATAATAATTTGGCATCACGAGacac > 1:4547428/1‑39 (MQ=255) tGAGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTg < 1:403425/71‑1 (MQ=255) tGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAatat < 1:1419566/51‑1 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAata > 1:120233/1‑48 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGa > 1:1795079/1‑70 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGa < 1:4491745/70‑1 (MQ=255) aGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAAtatta > 1:4129888/1‑51 (MQ=255) tcaatcaaTAATAATTTGGCATCACGAGACACATcac > 1:369433/1‑37 (MQ=255) tcaatcaaTAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGcaca < 1:4381842/57‑1 (MQ=255) caatcaatAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAg < 1:2808799/52‑1 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACagag > 1:3480692/1‑39 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAata > 1:4165056/1‑44 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACaa > 1:4244968/1‑62 (MQ=255) aatcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAt < 1:1701973/63‑1 (MQ=255) atcaatAATAATTTGGCATCACGAGACGCATcaca < 1:1072817/35‑1 (MQ=255) atcaatAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTg < 1:1286726/64‑1 (MQ=255) atcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGa > 1:2538542/1‑49 (MQ=255) tcaatAATAATTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTg < 1:3768331/63‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACaga < 1:4471813/36‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGa < 1:1255650/48‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGa < 1:3157535/64‑1 (MQ=255) tcaatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc < 1:1435928/71‑1 (MQ=255) tcaatAATAATTTGGCATAACGAGACACATCACAGAGGAATATTATGAGcac < 1:1754208/52‑1 (MQ=255) caatAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc > 1:2621703/1‑70 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGaa < 1:3914516/38‑1 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAg > 1:2746852/1‑52 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:1182077/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaa > 1:3637531/1‑71 (MQ=255) aataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCa < 1:2714079/70‑1 (MQ=255) ataataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAAAATTCaat < 1:1865298/71‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAata < 1:1459369/38‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaat < 1:2137825/70‑1 (MQ=255) taataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaat > 1:2195630/1‑70 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa < 1:344749/70‑1 (MQ=255) ataatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa < 1:2577911/70‑1 (MQ=255) aatTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAg > 1:2573337/1‑71 (MQ=255) aatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa < 1:820206/68‑1 (MQ=255) aatTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaataa < 1:1707211/68‑1 (MQ=255) atTTGGCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAata > 1:2724841/1‑59 (MQ=255) atTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAg < 1:3692614/70‑1 (MQ=255) atTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAAAAATAGt < 1:4074839/71‑1 (MQ=255) tGGCATCACGAGACACATCGCAGAGGAATATTATGAg > 1:3103488/1‑37 (MQ=255) gCATGACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:4302279/70‑1 (MQ=255) gCATCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:2808158/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:549620/70‑1 (MQ=255) gCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:4537672/70‑1 (MQ=255) aTCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatga < 1:2087889/69‑1 (MQ=255) aTCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAAt < 1:3198260/71‑1 (MQ=255) tCACGAGACGCATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATg > 1:1338546/1‑71 (MQ=255) tCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCaata < 1:4404357/58‑1 (MQ=255) tCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:1857331/67‑1 (MQ=255) tCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:447811/67‑1 (MQ=255) tCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGtgatg < 1:2051630/67‑1 (MQ=255) cACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAat > 1:3303903/1‑49 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACaa > 1:2860480/1‑41 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAAt > 1:1445409/1‑42 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGa > 1:4602503/1‑45 (MQ=255) aCGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAatat > 1:2705415/1‑50 (MQ=255) cGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGaa > 1:1959849/1‑45 (MQ=255) gagaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAat > 1:717566/1‑46 (MQ=255) gaCACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCaaa > 1:3994921/1‑71 (MQ=255) acacATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTc < 1:662118/47‑1 (MQ=255) | ATAATTTAAAAAAGATAAATATAAAAATATCAATGAGTCAATCAATAATAATTTGGCATCACGAGACACATCACAGAGGAATATTATGAGCACAGAAACAATTGAAATATTCAATAATAGTGATGAATGGGCAAA > W3110S.gb/2989839‑2989973 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |