| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,031,910 | A→G | 18.2% | N64D (AAC→GAC) | idi → | isopentenyl diphosphate isomerase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,031,910 | 0 | A | G | 18.2% | 97.1 / 16.5 | 44 | N64D (AAC→GAC) | idi | isopentenyl diphosphate isomerase |
| Reads supporting (aligned to +/- strand): ref base A (24/12); new base G (4/4); total (28/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
CCAAAGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTA > W3110S.gb/3031845‑3031977 | ccAAAGGACAATTATTAGTTACCCGCCGCGCACTTAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCg > 1:1665252/1‑71 (MQ=255)ccAAAGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCg > 1:2188761/1‑71 (MQ=255) aaaGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGt > 1:3951815/1‑71 (MQ=255) aGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGttt > 1:3771279/1‑71 (MQ=255) aGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGttt < 1:3116004/71‑1 (MQ=255) ttattaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCg > 1:3474295/1‑60 (MQ=255) ttaGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCAcc > 1:1684758/1‑70 (MQ=255) aCCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTtg > 1:4397973/1‑56 (MQ=255) ccgccgCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTgg < 1:2347566/71‑1 (MQ=255) gcgcACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGaa < 1:2428023/71‑1 (MQ=255) cACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTTGTGGGCACCcac < 1:2658174/57‑1 (MQ=255) aCTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGtt > 1:1379528/1‑43 (MQ=255) cTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTtgtg < 1:2005285/46‑1 (MQ=255) tGAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCgg < 1:2674625/39‑1 (MQ=255) gAGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTTGTGGTCACCCACAACTGGGAGAAAGCAAc > 1:4482476/1‑70 (MQ=255) gAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACaa > 1:1070818/1‑55 (MQ=255) gAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAAc > 1:4564760/1‑70 (MQ=255) aGCAAAAAAGCATGGCCTGGCGTGTGGACTGACTCGGTTTGTGGCCACCCACAACTGGGAGAAAGCAACg < 1:163381/70‑1 (MQ=255) aGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGc > 1:2748191/1‑46 (MQ=255) gCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGaa < 1:3947251/71‑1 (MQ=255) aaaaaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTgg > 1:1367806/1‑40 (MQ=255) aaaaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACg < 1:1013180/71‑1 (MQ=255) aaaGCATGGCCTGGCGTGTGGACTGACTCGGTTtg > 1:708342/1‑35 (MQ=255) aaaGCATGGCCTGGCGTGTGGACTAACTCGGTTtg > 1:677325/1‑35 (MQ=255) aaaGCATGGCCTGGCGTGTGGACTAACTCGGTTtg > 1:4575011/1‑35 (MQ=255) aaaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACg > 1:2056354/1‑70 (MQ=255) aaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGTCACCCACAACTGGGAGAAAGCAACGAAGACg > 1:789833/1‑69 (MQ=255) aaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCa > 1:1417890/1‑40 (MQ=255) aaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAAc > 1:3212379/1‑48 (MQ=255) aaGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACt < 1:2197909/49‑1 (MQ=255) aGCATGACCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAg > 1:596042/1‑71 (MQ=255) aTGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTg < 1:698827/70‑1 (MQ=255) aTGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGa < 1:2597967/71‑1 (MQ=255) gCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATcc > 1:2761236/1‑71 (MQ=255) ccTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATccg < 1:82780/71‑1 (MQ=255) tgtgGACTGACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATccgc < 1:186088/65‑1 (MQ=255) tgtgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGaa > 1:2344049/1‑50 (MQ=255) tgGACTGACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgttgccg > 1:4473645/1‑71 (MQ=255) tgGACTGACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgttgccg > 1:2121115/1‑71 (MQ=255) tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgttgccg > 1:817681/1‑71 (MQ=255) tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgttgcc > 1:2639617/1‑70 (MQ=255) tgGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgt < 1:2724659/66‑1 (MQ=255) gactgactCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgttgccgt > 1:2217666/1‑70 (MQ=255) gACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCgccgttgccgt < 1:1695768/70‑1 (MQ=255) aCTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTa > 1:3365833/1‑71 (MQ=255) | CCAAAGGACAATTATTAGTTACCCGCCGCGCACTGAGCAAAAAAGCATGGCCTGGCGTGTGGACTAACTCGGTTTGTGGGCACCCACAACTGGGAGAAAGCAACGAAGACGCAGTGATCCGCCGTTGCCGTTA > W3110S.gb/3031845‑3031977 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |