Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,053,087 T→A 15.9% Y137F (TAT→TTT)  pepP ← proline aminopeptidase P II

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,053,0870TA15.9% 106.5 / 13.9 44Y137F (TAT→TTT) pepPproline aminopeptidase P II
Reads supporting (aligned to +/- strand):  ref base T (20/17);  new base A (5/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTG  >  W3110S.gb/3053020‑3053155
                                                                   |                                                                    
tgcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg                                                                   <  1:648281/71‑1 (MQ=255)
tgcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg                                                                   <  1:339613/71‑1 (MQ=255)
 gcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg                                                                   >  1:3608201/1‑70 (MQ=255)
 gcggtgAGATTTTGCCGCGAAACTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg                                                                   >  1:1687020/1‑70 (MQ=255)
  cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg                                                                   >  1:3391320/1‑69 (MQ=255)
    gtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGTGCACTGTTCACGATTACATCAgcatatgcata                                                               <  1:3774349/71‑1 (MQ=255)
      gagaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATAtt                                                             >  1:974043/1‑71 (MQ=255)
      gagaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATAtt                                                             >  1:1125616/1‑71 (MQ=255)
      gagaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATAtt                                                             >  1:3321427/1‑71 (MQ=255)
       agaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTc                                                            >  1:1583754/1‑71 (MQ=255)
            ttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCCCTGTTCACGATTACATCAGCATATGCATATTCGCCCt                                                       >  1:4385350/1‑71 (MQ=255)
              gCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgcata                                                               <  1:4159911/61‑1 (MQ=255)
              gCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgcata                                                               <  1:372857/61‑1 (MQ=255)
               ccGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTggg                                                    <  1:555483/71‑1 (MQ=255)
               ccGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTggg                                                    <  1:1437428/71‑1 (MQ=255)
                cgcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATAtt                                                             >  1:1702330/1‑61 (MQ=255)
                 gcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa                                                  <  1:363029/71‑1 (MQ=255)
                 gcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa                                                  <  1:3626338/71‑1 (MQ=255)
                 gcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa                                                  <  1:3620865/71‑1 (MQ=255)
                  cgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCAt                                                 <  1:3178368/71‑1 (MQ=255)
                    aaCCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTg                                                      >  1:4052118/1‑64 (MQ=255)
                    aaCCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTg                                                      >  1:2869085/1‑64 (MQ=255)
                      ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGt                                              >  1:1734596/1‑70 (MQ=255)
                        tttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTaaa                                           >  1:3969297/1‑71 (MQ=255)
                         ttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgcatataa                                                            >  1:1039785/1‑51 (MQ=255)
                         ttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcata                                                                     >  1:2737221/1‑44 (MQ=255)
                         ttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcata                                                                     >  1:265313/1‑44 (MQ=255)
                                gTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc                                   <  1:1295966/71‑1 (MQ=255)
                                gTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc                                   <  1:1425400/71‑1 (MQ=255)
                                 tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGcc                                                         <  1:418464/48‑1 (MQ=255)
                                  ttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATAtt                                                             >  1:478437/1‑43 (MQ=255)
                                  ttttCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATgg                                               >  1:4283628/1‑57 (MQ=255)
                                   tttCCAGCGCACTGTTCACGATTACATCAgcatatgcata                                                               <  1:3390546/40‑1 (MQ=255)
                                   tttCCAGCGCACTGCTCACGATTACATCAgcatat                                                                    >  1:1913771/1‑35 (MQ=255)
                                    ttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGc                               >  1:4551421/1‑71 (MQ=255)
                                     tCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGcc                              >  1:534642/1‑71 (MQ=255)
                                           gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa                                                  >  1:2707233/1‑45 (MQ=255)
                                           gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa                                                  >  1:2632394/1‑45 (MQ=255)
                                           gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa                                                  >  1:3956584/1‑45 (MQ=255)
                                            cACTGTTCACGATTACATCAGCAAATGCATATTCg                                                           <  1:1025665/35‑1 (MQ=255)
                                             aCTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagta                      <  1:1329319/71‑1 (MQ=255)
                                              cTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagtag                     <  1:3058667/71‑1 (MQ=255)
                                               tGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa                         <  1:947230/66‑1 (MQ=255)
                                               tGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAag                        <  1:3722568/67‑1 (MQ=255)
                                                gTTCACGATTACATCAGCATATGCATATTCGCCCTgg                                                     >  1:3453347/1‑37 (MQ=255)
                                                gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACcac                                       >  1:1401480/1‑51 (MQ=255)
                                                gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACcac                                       >  1:2436232/1‑51 (MQ=255)
                                                gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACcac                                       >  1:1871224/1‑51 (MQ=255)
                                                 ttCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATc                                    >  1:1093529/1‑53 (MQ=255)
                                                   cACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGa                 >  1:3405693/1‑70 (MQ=255)
                                                    aCGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGa                 >  1:3930616/1‑69 (MQ=255)
                                                     cGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATaa              <  1:2021065/71‑1 (MQ=255)
                                                     cGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATaa              <  1:3350181/71‑1 (MQ=255)
                                                         tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGtt          >  1:2000718/1‑71 (MQ=255)
                                                           cATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTg         <  1:816415/70‑1 (MQ=255)
                                                             tCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt       <  1:216673/70‑1 (MQ=255)
                                                               aGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgat    <  1:2055908/71‑1 (MQ=255)
                                                                gcatatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgatt   <  1:3833810/71‑1 (MQ=255)
                                                                gcaaatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgatt   <  1:1409754/71‑1 (MQ=255)
                                                                 catatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattg  <  1:2135518/71‑1 (MQ=255)
                                                                   |                                                                    
TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTG  >  W3110S.gb/3053020‑3053155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: