| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,053,087 | T→A | 15.9% | Y137F (TAT→TTT) | pepP ← | proline aminopeptidase P II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,053,087 | 0 | T | A | 15.9% | 106.5 / 13.9 | 44 | Y137F (TAT→TTT) | pepP | proline aminopeptidase P II |
| Reads supporting (aligned to +/- strand): ref base T (20/17); new base A (5/2); total (25/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.80e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTG > W3110S.gb/3053020‑3053155 | tgcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg < 1:648281/71‑1 (MQ=255)tgcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg < 1:339613/71‑1 (MQ=255) gcggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg > 1:3608201/1‑70 (MQ=255) gcggtgAGATTTTGCCGCGAAACTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg > 1:1687020/1‑70 (MQ=255) cggtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatg > 1:3391320/1‑69 (MQ=255) gtgAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGTGCACTGTTCACGATTACATCAgcatatgcata < 1:3774349/71‑1 (MQ=255) gagaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATAtt > 1:974043/1‑71 (MQ=255) gagaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATAtt > 1:1125616/1‑71 (MQ=255) gagaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCAAATGCATAtt > 1:3321427/1‑71 (MQ=255) agaTTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTc > 1:1583754/1‑71 (MQ=255) ttGCCGCGAACCTTTACGCAGTTTTTCCAGCGCCCTGTTCACGATTACATCAGCATATGCATATTCGCCCt > 1:4385350/1‑71 (MQ=255) gCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgcata < 1:4159911/61‑1 (MQ=255) gCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgcata < 1:372857/61‑1 (MQ=255) ccGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTggg < 1:555483/71‑1 (MQ=255) ccGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTggg < 1:1437428/71‑1 (MQ=255) cgcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATAtt > 1:1702330/1‑61 (MQ=255) gcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa < 1:363029/71‑1 (MQ=255) gcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa < 1:3626338/71‑1 (MQ=255) gcgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa < 1:3620865/71‑1 (MQ=255) cgAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCAt < 1:3178368/71‑1 (MQ=255) aaCCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTg > 1:4052118/1‑64 (MQ=255) aaCCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTg > 1:2869085/1‑64 (MQ=255) ccTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGt > 1:1734596/1‑70 (MQ=255) tttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTaaa > 1:3969297/1‑71 (MQ=255) ttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcatatgcatataa > 1:1039785/1‑51 (MQ=255) ttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcata > 1:2737221/1‑44 (MQ=255) ttACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAgcata > 1:265313/1‑44 (MQ=255) gTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:1295966/71‑1 (MQ=255) gTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATcc < 1:1425400/71‑1 (MQ=255) tttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGcc < 1:418464/48‑1 (MQ=255) ttttCCAGCGCACTGTTCACGATTACATCAGCATATGCATAtt > 1:478437/1‑43 (MQ=255) ttttCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATgg > 1:4283628/1‑57 (MQ=255) tttCCAGCGCACTGTTCACGATTACATCAgcatatgcata < 1:3390546/40‑1 (MQ=255) tttCCAGCGCACTGCTCACGATTACATCAgcatat > 1:1913771/1‑35 (MQ=255) ttCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGc > 1:4551421/1‑71 (MQ=255) tCCAGCGCACTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGcc > 1:534642/1‑71 (MQ=255) gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:2707233/1‑45 (MQ=255) gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:2632394/1‑45 (MQ=255) gcACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCa > 1:3956584/1‑45 (MQ=255) cACTGTTCACGATTACATCAGCAAATGCATATTCg < 1:1025665/35‑1 (MQ=255) aCTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagta < 1:1329319/71‑1 (MQ=255) cTGTTCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAagtag < 1:3058667/71‑1 (MQ=255) tGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTaa < 1:947230/66‑1 (MQ=255) tGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAag < 1:3722568/67‑1 (MQ=255) gTTCACGATTACATCAGCATATGCATATTCGCCCTgg > 1:3453347/1‑37 (MQ=255) gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACcac > 1:1401480/1‑51 (MQ=255) gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACcac > 1:2436232/1‑51 (MQ=255) gTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACcac > 1:1871224/1‑51 (MQ=255) ttCACGATTACATCAGCAAATGCATATTCGCCCTGGGCATGGTAAACCACATc > 1:1093529/1‑53 (MQ=255) cACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGa > 1:3405693/1‑70 (MQ=255) aCGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGa > 1:3930616/1‑69 (MQ=255) cGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATaa < 1:2021065/71‑1 (MQ=255) cGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATaa < 1:3350181/71‑1 (MQ=255) tACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGtt > 1:2000718/1‑71 (MQ=255) cATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTg < 1:816415/70‑1 (MQ=255) tCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCt < 1:216673/70‑1 (MQ=255) aGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgat < 1:2055908/71‑1 (MQ=255) gcatatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgatt < 1:3833810/71‑1 (MQ=255) gcaaatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgatt < 1:1409754/71‑1 (MQ=255) catatgcatatTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCtgattg < 1:2135518/71‑1 (MQ=255) | TGCGGTGAGATTTTGCCGCGAACCTTTACGCAGTTTTTCCAGCGCACTGTTCACGATTACATCAGCATATGCATATTCGCCCTGGGCATGGTAAACCACATCCAGGCCGTTAAGTAGTTGATAAAGTTGCTGATTG > W3110S.gb/3053020‑3053155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |