| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,062,130 | A→C | 32.7% | E163A (GAA→GCA) | argK → | membrane ATPase/protein kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,062,130 | 0 | A | C | 32.7% | 23.4 / 52.6 | 49 | E163A (GAA→GCA) | argK | membrane ATPase/protein kinase |
| Reads supporting (aligned to +/- strand): ref base A (17/16); new base C (10/6); total (27/22) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.29e-01 | |||||||||||
TTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCAT > W3110S.gb/3062063‑3062199 | ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACATaa < 1:377833/69‑1 (MQ=255)ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGaa < 1:2460665/69‑1 (MQ=255)ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAg < 1:912405/70‑1 (MQ=255)ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAg < 1:1177928/70‑1 (MQ=255)ttATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAg < 1:37691/70‑1 (MQ=255) gAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTAGCCCg < 1:2048450/71‑1 (MQ=255) gAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCg < 1:4269628/71‑1 (MQ=255) gAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCg < 1:1556613/71‑1 (MQ=255) agcagcGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGaa < 1:3480494/61‑1 (MQ=255) gcatcGGGTTATGACGTAGTGACTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAt < 1:2819667/71‑1 (MQ=255) gcagcGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCAt > 1:4227289/1‑71 (MQ=255) gggTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtggtg > 1:704144/1‑70 (MQ=255) gTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGc < 1:3009733/58‑1 (MQ=255) gTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGAc < 1:3008465/71‑1 (MQ=255) tGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGtt > 1:4188920/1‑71 (MQ=255) gACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGttt > 1:2739332/1‑71 (MQ=255) cGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGt > 1:3545655/1‑48 (MQ=255) gTAGTGATTGTCGAAACGGTTGGCGTCGGGCTGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTAt < 1:2488859/71‑1 (MQ=255) tAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAg < 1:2993573/45‑1 (MQ=255) tAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCg < 1:3876731/52‑1 (MQ=255) aGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGt > 1:130919/1‑45 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGaa > 1:1988052/1‑42 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTc > 1:1262335/1‑45 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTc > 1:36492/1‑45 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCa > 1:3338153/1‑52 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCa > 1:3636095/1‑52 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTAtctc > 1:1475108/1‑71 (MQ=255) gTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTAt > 1:2668877/1‑68 (MQ=255) tGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAAcagcag < 1:3915160/42‑1 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtgg > 1:4297305/1‑52 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtgg > 1:60674/1‑52 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCAtgg > 1:1994006/1‑52 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGtt > 1:1270788/1‑71 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGtt > 1:2611717/1‑71 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGt < 1:1091782/70‑1 (MQ=255) aTTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGt < 1:1442840/70‑1 (MQ=255) gTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGcc > 1:3831647/1‑42 (MQ=255) tCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCa < 1:2768926/70‑1 (MQ=255) aaaCGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAAtt < 1:540354/71‑1 (MQ=255) cGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGcc < 1:1057290/71‑1 (MQ=255) gTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCgg > 1:4588203/1‑71 (MQ=255) tCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCaaa > 1:2190195/1‑57 (MQ=255) tCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCaa > 1:2195476/1‑56 (MQ=255) cAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCgatga < 1:3330586/71‑1 (MQ=255) gTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATc < 1:2463058/71‑1 (MQ=255) tCGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTggcggc < 1:1182223/63‑1 (MQ=255) cGGAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTg > 1:642564/1‑71 (MQ=255) ggAAACAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCga > 1:1112508/1‑63 (MQ=255) gAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGc < 1:2570818/70‑1 (MQ=255) aaCAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAgg > 1:2623441/1‑71 (MQ=255) aCAGCAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAggg < 1:4296134/71‑1 (MQ=255) aCAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTgg > 1:1787433/1‑53 (MQ=255) aCAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAggg > 1:2036848/1‑71 (MQ=255) gAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCAt > 1:2884660/1‑71 (MQ=255) | TTATGCGAAGCAGCGGGTTATGACGTAGTGATTGTCGAAACGGTTGGCGTCGGGCAGTCGGAAACAGAAGTCGCCCGCATGGTGGACTGTTTTATCTCGTTGCAAATTGCCGGTGGCGGCGATGATCTGCAGGGCAT > W3110S.gb/3062063‑3062199 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |