| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,100,981 | A→G | 15.1% | H176H (CAT→CAC) | yggH ← | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,100,981 | 0 | A | G | 15.1% | 130.5 / 15.7 | 53 | H176H (CAT→CAC) | yggH | tRNA (m7G46) methyltransferase, SAM‑dependent |
| Reads supporting (aligned to +/- strand): ref base A (15/30); new base G (5/3); total (20/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
TTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGA > W3110S.gb/3100913‑3101042 | ttATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCatat < 1:2372139/70‑1 (MQ=255) tataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATg < 1:3586919/70‑1 (MQ=255) tataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATg < 1:1655768/70‑1 (MQ=255) tataACCGTCAATAGAAGACATCACTT‑AAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:4438110/70‑1 (MQ=255) ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTgg < 1:4465611/70‑1 (MQ=255) ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:4113034/70‑1 (MQ=255) ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1194481/70‑1 (MQ=255) ataACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:4130071/70‑1 (MQ=255) taACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa < 1:3868592/71‑1 (MQ=255) gTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1526442/64‑1 (MQ=255) tCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1633505/63‑1 (MQ=255) tCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATg < 1:3822450/62‑1 (MQ=255) tCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGa > 1:2784556/1‑64 (MQ=255) aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcccc > 1:896594/1‑71 (MQ=255) aaTAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGcccc > 1:196106/1‑71 (MQ=255) gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATa > 1:3459576/1‑61 (MQ=255) gaagaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCa > 1:1957175/1‑70 (MQ=255) agaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAg > 1:2209907/1‑69 (MQ=255) gaCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTg < 1:1028174/71‑1 (MQ=255) aCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:4434772/53‑1 (MQ=255) aCATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg < 1:1942163/53‑1 (MQ=255) cATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATgg > 1:2134012/1‑52 (MQ=255) aTCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCGAGCTGCAg < 1:1295796/71‑1 (MQ=255) aTCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAg < 1:3823489/71‑1 (MQ=255) ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGAATa > 1:3430020/1‑50 (MQ=255) ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGAATa > 1:370726/1‑50 (MQ=255) ttCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGAATACGc > 1:56252/1‑53 (MQ=255) tCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCt > 1:2823013/1‑71 (MQ=255) aaGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATGTGGAATACGCCCCCCAGCTGCAGTTTGCtt < 1:1610054/70‑1 (MQ=255) aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGGtttt < 1:4494626/71‑1 (MQ=255) aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCtttt < 1:3444027/71‑1 (MQ=255) aGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCtttt < 1:2201667/71‑1 (MQ=255) gCATATGTTCCGCATAAGGTTCCCAGTCGGGCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCttttt < 1:4103853/71‑1 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATa < 1:1252334/42‑1 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCttttt < 1:2443504/68‑1 (MQ=255) tatGTTCCGCATAAGGTTCCCAGTCGGGCGCCATATGGaa < 1:630372/40‑1 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATTGAATACGCCCCCCAGCTGCAGTTTGc > 1:978578/1‑61 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa > 1:3130218/1‑38 (MQ=255) tGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGaa > 1:2432237/1‑38 (MQ=255) ccGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTc > 1:3340186/1‑70 (MQ=255) ccGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCg > 1:1026731/1‑71 (MQ=255) ccGCATAAGCTTCCCAGTCGGTCGCCATGTGGAATa > 1:4524356/1‑36 (MQ=37) cATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCa < 1:1030012/71‑1 (MQ=255) ccAGTCCGGCGCCATGTGGAATACGCCCCCCAGCTGCAGTTTg < 1:4483750/43‑1 (MQ=37) aGTCGGTCGCCATGTTGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTg > 1:3003371/1‑70 (MQ=255) aGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGc < 1:4381890/58‑1 (MQ=255) gtcggtcgCCATATGGAATACGCCCCACAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGaa > 1:672699/1‑71 (MQ=255) tcggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCtt < 1:721124/42‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:3672944/71‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa > 1:1057507/1‑71 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:449974/71‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:3329166/71‑1 (MQ=255) ggtcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:3925331/71‑1 (MQ=255) tcgCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGa < 1:1024117/69‑1 (MQ=255) atatGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCa < 1:4533274/48‑1 (MQ=255) | TTATAACCGTCAATAGAAGACATCACTTCAAGCATATGTTCCGCATAAGGTTCCCAGTCGGTCGCCATATGGAATACGCCCCCCAGCTGCAGTTTGCTTTTTACCAGTTCGGCAAACGGCACCTGAACGA > W3110S.gb/3100913‑3101042 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |