| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,104,160 | C→T | 36.6% | R358C (CGC→TGC) | mltC → | membrane‑bound lytic murein transglycosylase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,104,160 | 0 | C | T | 36.6% | 37.2 / 38.3 | 41 | R358C (CGC→TGC) | mltC | membrane‑bound lytic murein transglycosylase C |
| Reads supporting (aligned to +/- strand): ref base C (13/13); new base T (11/4); total (24/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.95e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
CGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCAC > W3110S.gb/3104094‑3104216 | cGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTAccgccgccg < 1:104172/71‑1 (MQ=255) gCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGa > 1:1310548/1‑71 (MQ=255) ccATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGAt < 1:4597256/71‑1 (MQ=255) ccTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTc > 1:4161377/1‑71 (MQ=255) ctGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCa > 1:229592/1‑70 (MQ=255) tGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCAt > 1:3795566/1‑70 (MQ=255) cGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCAtt < 1:141901/69‑1 (MQ=255) cGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTaa > 1:1584939/1‑71 (MQ=255) ggAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCAtt < 1:3199261/68‑1 (MQ=255) aaTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAAc < 1:2502685/69‑1 (MQ=255) aTCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGc > 1:4417123/1‑71 (MQ=255) gCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCt > 1:3516852/1‑70 (MQ=255) gCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCt > 1:774501/1‑70 (MQ=255) gCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCt < 1:3237309/70‑1 (MQ=255) ttATCTTTATATAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTc < 1:4169049/52‑1 (MQ=255) aTCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATg > 1:1067588/1‑71 (MQ=255) tCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTcc > 1:2173656/1‑50 (MQ=255) tttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGAt < 1:2018863/42‑1 (MQ=255) ttATAAAGTGAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATGCt > 1:3655945/1‑69 (MQ=255) ttATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCt > 1:3728589/1‑69 (MQ=255) tataAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTc < 1:3676703/69‑1 (MQ=255) tGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaa < 1:2601301/71‑1 (MQ=255) gAATACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGa < 1:431216/53‑1 (MQ=255) aaTACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCaaaaa > 1:1494050/1‑71 (MQ=255) aaTACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCg > 1:2509055/1‑45 (MQ=255) aTACCGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACg > 1:719016/1‑53 (MQ=255) tACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGa < 1:725517/53‑1 (MQ=255) aCCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGt > 1:2788112/1‑71 (MQ=255) ccGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATGCTc > 1:3253355/1‑56 (MQ=255) ccGCGCAAAAATCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGACGATGCTc > 1:1257866/1‑56 (MQ=255) ccGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGc > 1:812004/1‑42 (MQ=255) ccGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATg > 1:987568/1‑69 (MQ=255) ccGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGt > 1:4312130/1‑70 (MQ=255) ccGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGt < 1:3177339/70‑1 (MQ=255) cgcgCAAAAATCCTACCGCTGCCGATAATTGCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTa > 1:4242914/1‑71 (MQ=255) gcgcAAAAATCCTACCGCTGCCGATAATTCCATTAAc > 1:2014856/1‑37 (MQ=255) cgcAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATc > 1:551889/1‑71 (MQ=255) aaaaaTCCTACCGCTGCCGATAATTCCATTAACCGCCCCTGAc < 1:558571/43‑1 (MQ=255) aaaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCcac < 1:2995153/71‑1 (MQ=255) aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAc < 1:4318647/42‑1 (MQ=255) aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGAc < 1:1004997/42‑1 (MQ=255) aaaaTCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTc < 1:3347018/49‑1 (MQ=255) | CGCCATCCCTCTGCGGAATCTCGCCGTTATCTTTATAAAGTGAATACCGCGCAAAAATCCTACCGCCGCCGATAATTCCATTAACCGCCCCTGACGATGCTCAGGGGCAAAAATGTTATCCAC > W3110S.gb/3104094‑3104216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |