| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,123,192 | A→C | 8.1% | C308W (TGT→TGG) | glcF ← | glycolate oxidase iron‑sulfur subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,123,192 | 0 | A | C | 8.1% | 144.6 / 7.4 | 61 | C308W (TGT→TGG) | glcF | glycolate oxidase iron‑sulfur subunit |
| Reads supporting (aligned to +/- strand): ref base A (23/33); new base C (3/2); total (26/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGA > W3110S.gb/3123128‑3123261 | tAAATCCAAGACGAAGCAACACTTTTACCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACggg > 1:3791145/1‑70 (MQ=255) aaaTCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTaca > 1:1586312/1‑66 (MQ=255) aaTCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGa > 1:765401/1‑69 (MQ=255) aaTCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGa > 1:770843/1‑69 (MQ=255) aaTCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGAc < 1:1965512/70‑1 (MQ=255) aaTCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGAc < 1:3474093/70‑1 (MQ=255) tCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGt > 1:2994082/1‑71 (MQ=255) cAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTg > 1:1764279/1‑70 (MQ=255) aaGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTgg < 1:3455857/70‑1 (MQ=255) gACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGa > 1:799402/1‑62 (MQ=255) cGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGc > 1:3481691/1‑71 (MQ=255) aaGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTa < 1:1889642/52‑1 (MQ=255) aaGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTCCACGGACAGTGGAAGGc > 1:1028321/1‑69 (MQ=255) aaGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAg < 1:132112/61‑1 (MQ=255) aaGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTT‑TAGGGTACACGGACAg < 1:4575590/60‑1 (MQ=255) aGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAg > 1:3915086/1‑60 (MQ=255) cAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGa < 1:1241887/55‑1 (MQ=255) cacTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGaa < 1:4471999/60‑1 (MQ=255) cacTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCtttt < 1:3317475/71‑1 (MQ=255) cacTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCtttt < 1:3137949/71‑1 (MQ=255) cacTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCtt > 1:2775284/1‑69 (MQ=255) acTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCttttt < 1:2767647/71‑1 (MQ=255) acTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCttttt < 1:3929068/71‑1 (MQ=255) cTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTa < 1:2719567/44‑1 (MQ=255) tttCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTa > 1:4293137/1‑41 (MQ=255) aCTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTCCACGGACAGTGGAAGGCCAGCTTTTTATCGCcgcg < 1:4465355/71‑1 (MQ=255) aCTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTg < 1:2615024/47‑1 (MQ=255) aCTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATcgccggg > 1:732858/1‑69 (MQ=255) aCTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCcgcg < 1:3711031/71‑1 (MQ=255) ttCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACg < 1:3956885/38‑1 (MQ=255) ttCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGAc < 1:4525865/41‑1 (MQ=255) tCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTCCACGGACa > 1:3687796/1‑41 (MQ=255) cGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGaa < 1:582888/69‑1 (MQ=255) cGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAAt < 1:4335685/70‑1 (MQ=255) cGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAAt < 1:4509323/70‑1 (MQ=255) ccGTTCAGCTTTTGCGCATGTTGTAGGGTCCACGGACAGTGGAAGGCCAGCttt < 1:3993614/54‑1 (MQ=255) ccGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCtt < 1:4558495/53‑1 (MQ=255) cGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGa < 1:2640381/35‑1 (MQ=255) cGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGa < 1:2167049/35‑1 (MQ=255) gTTCAGCTTTTGCGCATGTTGTAGGGTCCACGGACAGTGGAAGGCCAGCtttt > 1:3428480/1‑53 (MQ=255) ttCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGcc < 1:4153304/45‑1 (MQ=255) ttCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCtt < 1:1796826/50‑1 (MQ=255) cAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCtttt > 1:1810465/1‑50 (MQ=255) cAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGtt > 1:3151627/1‑71 (MQ=255) aGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGttt < 1:1037212/71‑1 (MQ=255) ttttGCGCATGTTGTAGGGTACACGGACAGTGGaa > 1:1060619/1‑35 (MQ=255) tttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCttttt > 1:1911902/1‑46 (MQ=255) ttGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTc < 1:3207269/69‑1 (MQ=255) cgcATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGc < 1:1442418/70‑1 (MQ=255) gcATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCgg < 1:2956589/71‑1 (MQ=255) ttgtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGaa > 1:4212770/1‑47 (MQ=255) ttgtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCt > 1:4512171/1‑71 (MQ=255) ttgtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCt > 1:4375267/1‑71 (MQ=255) tgtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCcgcg < 1:4486960/44‑1 (MQ=255) gtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAAt > 1:3199527/1‑46 (MQ=255) gtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAAt > 1:2070668/1‑46 (MQ=255) gtAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAAt > 1:3954917/1‑46 (MQ=255) tAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGaa > 1:3021456/1‑44 (MQ=255) tAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGaa > 1:701167/1‑44 (MQ=255) ggTACACGGACAGTGGAAGGCCAGCTTTTTATCGCcgcg < 1:2898964/39‑1 (MQ=255) gTACACGGACAGTGGAAGGCCAGCTTTTTATCGCcgcg < 1:1829827/38‑1 (MQ=255) tACACGGACTGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGa < 1:700302/71‑1 (MQ=255) tACACGGACAGTGGAAGGCCAGCTTTTTATCGCcgcg < 1:875435/37‑1 (MQ=255) tACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGaa > 1:3236029/1‑39 (MQ=255) tACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGa < 1:402882/38‑1 (MQ=255) | TAAATCCAAGACGAAGCAACACTTTTTCCACTTCGCCGTTCAGCTTTTGCGCATGTTGTAGGGTACACGGACAGTGGAAGGCCAGCTTTTTATCGCCGCGAATTGCCAGTTTTTCCAGCGGTTCCTCGCGCAGA > W3110S.gb/3123128‑3123261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |