| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,128,408 | T→C | 25.7% | D155G (GAC→GGC) | yghO ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,128,408 | 0 | T | C | 25.7% | 122.1 / 49.5 | 74 | D155G (GAC→GGC) | yghO | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (21/34); new base C (5/14); total (26/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.14e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.55e-01 | |||||||||||
TAACCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGC > W3110S.gb/3128339‑3128478 | tAACCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTc < 1:2546889/71‑1 (MQ=255) aCCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaa < 1:896080/71‑1 (MQ=255) aCCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaa < 1:1644942/71‑1 (MQ=255) aCCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCa > 1:447401/1‑70 (MQ=255) ccAGTTATTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaaaa > 1:3140074/1‑71 (MQ=255) gttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc > 1:2716371/1‑70 (MQ=255) gttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc > 1:3042197/1‑70 (MQ=255) gttCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc > 1:1511797/1‑70 (MQ=255) tCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:454333/1‑71 (MQ=255) cAATATGCGCGGCATTCCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:2396814/70‑1 (MQ=255) cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:1410784/1‑70 (MQ=255) cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:3026025/1‑70 (MQ=255) cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:1010685/1‑70 (MQ=255) cAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:1170702/1‑70 (MQ=255) atatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCaaa < 1:3021170/59‑1 (MQ=255) atatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:1547825/71‑1 (MQ=255) atatGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAg < 1:3134353/71‑1 (MQ=255) tGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTa > 1:2877669/1‑70 (MQ=255) gcgGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATcc < 1:268364/71‑1 (MQ=255) ggCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAACCCTTCAATCAGTAATCCGc < 1:1847164/71‑1 (MQ=255) ggCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGc < 1:1423599/71‑1 (MQ=255) tACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTc < 1:3416303/53‑1 (MQ=255) tACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:2267246/56‑1 (MQ=255) tACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:1253135/56‑1 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:4606467/71‑1 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:3801991/1‑71 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:1260114/1‑71 (MQ=255) ccACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt > 1:3928427/1‑71 (MQ=255) aCGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt > 1:2590085/1‑52 (MQ=255) ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:486226/53‑1 (MQ=255) ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:605958/53‑1 (MQ=255) ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:256894/53‑1 (MQ=255) ggTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:316068/1‑71 (MQ=255) gctttGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAg < 1:2223768/51‑1 (MQ=255) gTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgattg < 1:4591705/71‑1 (MQ=255) gTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:4442749/1‑70 (MQ=255) gTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCt < 1:3857442/66‑1 (MQ=255) gCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGata < 1:3258283/70‑1 (MQ=255) ccGTGTGGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATAtt < 1:223352/71‑1 (MQ=255) gtgtGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga < 1:536490/65‑1 (MQ=255) tgtgGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAAt < 1:2117457/41‑1 (MQ=255) tgGCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga > 1:619582/1‑62 (MQ=255) gCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtga > 1:2553946/1‑56 (MQ=255) gCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCt > 1:827862/1‑70 (MQ=255) gCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTg < 1:422615/71‑1 (MQ=255) gCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTg < 1:3286354/71‑1 (MQ=255) gCATCATCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTg < 1:2172188/71‑1 (MQ=255) catcatCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:2528472/71‑1 (MQ=255) catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:1992104/71‑1 (MQ=255) catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:3239354/71‑1 (MQ=255) catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:2279645/71‑1 (MQ=255) catcatCGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:947044/71‑1 (MQ=255) atcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:1652881/71‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTaa < 1:909812/40‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTa < 1:2494456/39‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCt < 1:1390788/46‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:1200295/70‑1 (MQ=255) tcatcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:4439608/70‑1 (MQ=255) catcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga < 1:2388035/56‑1 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgat < 1:4067205/52‑1 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCtgat < 1:4106111/52‑1 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGata > 1:2815054/1‑57 (MQ=255) atcGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg > 1:4006463/1‑70 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTa < 1:3163705/37‑1 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTa < 1:2053007/37‑1 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga > 1:1333370/1‑55 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATAtt > 1:4082219/1‑59 (MQ=255) atcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg < 1:4407743/70‑1 (MQ=255) atcGCACAGGGGGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAgg < 1:3357568/71‑1 (MQ=255) tcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTaa < 1:481859/37‑1 (MQ=255) tcGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGa < 1:736359/66‑1 (MQ=255) cGCACAGGGTGGTGTGCCAAAACCTTCAATCAGTAAt > 1:4447979/1‑37 (MQ=255) aGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTg < 1:995930/71‑1 (MQ=255) ggtggtGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGAt > 1:4334674/1‑71 (MQ=255) gtgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGaa < 1:3402387/55‑1 (MQ=255) tgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAgg < 1:3450088/57‑1 (MQ=255) tgtgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTAc < 1:3082554/71‑1 (MQ=255) tgtCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAg < 1:2834470/54‑1 (MQ=255) tCAAAACCTTCAATCAGTAATCCGCTTTCCtgattga < 1:837537/37‑1 (MQ=255) tCAAAACCTTCAATCAGTAATCCGCTTTCCtgatt > 1:209960/1‑35 (MQ=255) tCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACtt > 1:739633/1‑69 (MQ=255) tCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTg > 1:333334/1‑70 (MQ=255) tCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGc < 1:1843229/71‑1 (MQ=255) | TAACCCAGTTGTTCAATATGCGCGGCATACCACGGTTTGCCGTGTGGCATCATCGCACAGGGTGGTGTGTCAAAACCTTCAATCAGTAATCCGCTTTCCTGATTGATATTCAGGCTGAAAGGACCGCTGATCTTACTTGC > W3110S.gb/3128339‑3128478 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |