| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,141,409 | C→T | 12.6% | A79A (GCG→GCA) | hybC ← | hydrogenase 2, large subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,141,409 | 0 | C | T | 12.6% | 266.7 / 24.1 | 95 | A79A (GCG→GCA) | hybC | hydrogenase 2, large subunit |
| Reads supporting (aligned to +/- strand): ref base C (46/37); new base T (6/6); total (52/43) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.64e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
ATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCAC > W3110S.gb/3141340‑3141477 | aTGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAgcg < 1:3068077/71‑1 (MQ=255) tGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAgtgc < 1:1756499/71‑1 (MQ=255) cGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCAc > 1:2382887/1‑71 (MQ=255) cGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCAc > 1:69265/1‑71 (MQ=255) gtggtgTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACt < 1:3958026/71‑1 (MQ=255) gtgtGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTc < 1:4387133/71‑1 (MQ=255) gtgtGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTc < 1:4168451/71‑1 (MQ=255) gtgtGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTc < 1:3355649/71‑1 (MQ=255) tgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTg > 1:3671260/1‑70 (MQ=255) tgCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGc > 1:2610042/1‑71 (MQ=255) cgcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCg > 1:4600842/1‑71 (MQ=255) cgcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGcc > 1:3225444/1‑70 (MQ=255) cgcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCg > 1:1489630/1‑71 (MQ=255) cgcAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCg > 1:3855898/1‑71 (MQ=255) ccAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTg < 1:2412401/63‑1 (MQ=255) cAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGaa > 1:4087333/1‑71 (MQ=255) cAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGa < 1:2233004/70‑1 (MQ=255) aGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGcc > 1:4325088/1‑63 (MQ=255) aGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAAc > 1:664196/1‑71 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg < 1:3149561/71‑1 (MQ=255) aaTGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACg > 1:196520/1‑70 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGcc > 1:2953691/1‑60 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGcc > 1:2921319/1‑60 (MQ=255) atgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:422513/1‑70 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCATCGCACTTTCTGCCGCACGAACgg > 1:4642513/1‑69 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:2981939/1‑69 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:1000434/1‑69 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:3279507/71‑1 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:2375389/71‑1 (MQ=255) tgatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:176875/71‑1 (MQ=255) gatgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACgg > 1:28657/1‑68 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAgagc > 1:1999933/1‑47 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACttt > 1:3501417/1‑52 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACttt > 1:907104/1‑52 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGa > 1:3806878/1‑71 (MQ=255) atgTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGa < 1:2446318/71‑1 (MQ=255) ttACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:3468759/66‑1 (MQ=255) aCGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACt > 1:3312786/1‑45 (MQ=255) cGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAgcgc > 1:4513776/1‑71 (MQ=255) gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt < 1:2218711/71‑1 (MQ=255) gATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt < 1:2135509/71‑1 (MQ=255) aTGTATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:3318218/71‑1 (MQ=255) tGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACg < 1:868928/52‑1 (MQ=255) tGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt < 1:2988867/69‑1 (MQ=255) gTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACt > 1:3540421/1‑39 (MQ=255) gTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGa < 1:802615/60‑1 (MQ=255) tATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACg > 1:3673716/1‑50 (MQ=255) tATTGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACg > 1:328522/1‑50 (MQ=255) aTTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTg > 1:1472606/1‑71 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg < 1:3810600/71‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg < 1:4096009/71‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCa > 1:3364863/1‑46 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGaa < 1:2247739/55‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt < 1:1051011/65‑1 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:158492/1‑71 (MQ=255) ttGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg < 1:3492300/71‑1 (MQ=255) tGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGcgcg > 1:2646189/1‑63 (MQ=255) tGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGcgcg > 1:699328/1‑63 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg > 1:828054/1‑66 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:4058378/1‑69 (MQ=255) gcgcGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:1353090/1‑69 (MQ=255) gcgcGTTAACCGGAACGTCGATATCCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg > 1:1749274/1‑66 (MQ=255) cgcgTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgta < 1:3197250/70‑1 (MQ=255) gTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTAc < 1:523455/71‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtagta < 1:4535210/69‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa < 1:2873408/71‑1 (MQ=255) ttAACCGGAACGTCGATATTCAGCGCACTTTCTGACGCACGAACGGAAGACAGCGCGTGAGTGgt < 1:3645211/65‑1 (MQ=255) tAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGaa > 1:148393/1‑42 (MQ=255) aaCCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGaa > 1:937665/1‑41 (MQ=255) aaCCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACAt < 1:4449104/70‑1 (MQ=255) aaCCGGAACGTCGATATTCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATa > 1:1031575/1‑71 (MQ=255) aaCCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACAt < 1:3690190/70‑1 (MQ=255) aaCCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATa > 1:3269936/1‑71 (MQ=255) aaCCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATa > 1:3264396/1‑71 (MQ=255) ccGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGaa > 1:2527896/1‑39 (MQ=255) ccGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGaa > 1:4120282/1‑39 (MQ=255) ccGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg < 1:1680862/57‑1 (MQ=255) ccGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt < 1:3193595/61‑1 (MQ=255) cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:3429517/54‑1 (MQ=255) cGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc < 1:1000796/71‑1 (MQ=255) ggAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTg < 1:1326966/53‑1 (MQ=255) ggAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc > 1:1073989/1‑70 (MQ=255) ggAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGc > 1:3269958/1‑70 (MQ=255) aCGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACa > 1:999912/1‑43 (MQ=255) aCGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGt > 1:72254/1‑49 (MQ=255) gTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGa < 1:4035859/71‑1 (MQ=255) tCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtagta < 1:1826006/57‑1 (MQ=255) gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGa > 1:4070688/1‑46 (MQ=255) gATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtag < 1:788438/53‑1 (MQ=255) atatTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgt < 1:868900/50‑1 (MQ=255) ttCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAg < 1:2120232/43‑1 (MQ=255) ttCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGa < 1:1925730/64‑1 (MQ=255) tCAGTGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTgg > 1:2760775/1‑45 (MQ=255) cAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgta > 1:3644145/1‑46 (MQ=255) cAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACa > 1:3134581/1‑51 (MQ=255) cAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGca > 1:2589706/1‑71 (MQ=255) aGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGcac < 1:2571726/71‑1 (MQ=255) aGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGcac < 1:1770267/71‑1 (MQ=255) cgcACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGgtagt < 1:3021071/45‑1 (MQ=255) cgcACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCcaca < 1:2578560/57‑1 (MQ=255) | ATGCGTGGTGTGCGCAGCCAGAATGATGTTACGGATGTATTGCGCGTTAACCGGAACGTCGATATTCAGCGCACTTTCTGCCGCACGAACGGAAGACAGCGCGTGAGTGGTAGTACATACGCCACAGATACGTTGCAC > W3110S.gb/3141340‑3141477 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |