| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,163,647 | T→A | 9.4% | N328I (AAC→ATC) | parC ← | DNA topoisomerase IV, subunit A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,163,647 | 0 | T | A | 9.4% | 139.4 / 7.3 | 53 | N328I (AAC→ATC) | parC | DNA topoisomerase IV, subunit A |
| Reads supporting (aligned to +/- strand): ref base T (28/20); new base A (2/3); total (30/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.25e-01 | |||||||||||
AGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCA > W3110S.gb/3163578‑3163713 | aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGtt < 1:240062/71‑1 (MQ=255)aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGtt < 1:3572971/71‑1 (MQ=255)aGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGtt < 1:2111358/71‑1 (MQ=255) cGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTaa > 1:1741878/1‑66 (MQ=255) aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATAc < 1:603657/61‑1 (MQ=255) aTTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATAc < 1:3132098/61‑1 (MQ=255) ttCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCtttt > 1:3940733/1‑71 (MQ=255) ccAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTc < 1:2948356/70‑1 (MQ=255) ccAGCAAGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTc > 1:601052/1‑70 (MQ=255) gcagGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTaa > 1:1217351/1‑51 (MQ=255) gTTTTTCACCGCCTGACGACCATCCAGACCGATCATATTAAGGTTaa < 1:3279923/47‑1 (MQ=255) ttttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGtt < 1:3521735/43‑1 (MQ=255) tttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGa > 1:4230556/1‑49 (MQ=255) ttCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCggtgg > 1:3869093/1‑70 (MQ=255) ccgccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCg < 1:1345291/71‑1 (MQ=255) gccgGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAg < 1:1030178/67‑1 (MQ=255) ggACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAagag > 1:1887595/1‑71 (MQ=255) ggACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAagag > 1:1734348/1‑71 (MQ=255) gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTc < 1:2321597/49‑1 (MQ=255) gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 1:2907652/71‑1 (MQ=255) gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 1:3476689/71‑1 (MQ=255) gacgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg < 1:4614939/71‑1 (MQ=255) cgacCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggtg < 1:2494672/71‑1 (MQ=255) ccATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCgg > 1:2130404/1‑52 (MQ=255) cATCCAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCgg < 1:2896073/51‑1 (MQ=255) aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAt < 1:1793697/47‑1 (MQ=255) aTCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGAt < 1:2030519/47‑1 (MQ=255) tCCAGACCGATCATATTAAGGTTAATACGATAGctc < 1:1721569/36‑1 (MQ=255) ccAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcat > 1:4514695/1‑70 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCa > 1:2060961/1‑41 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCa > 1:3429944/1‑41 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAggt > 1:667994/1‑62 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:1339804/1‑71 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:3458393/1‑71 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatca > 1:196477/1‑71 (MQ=255) cAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatc < 1:2664572/70‑1 (MQ=255) cAGACCGATCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTtcatc < 1:780806/70‑1 (MQ=255) aGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa < 1:2920888/56‑1 (MQ=255) ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGaa > 1:2273836/1‑53 (MQ=255) ccGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGtt > 1:171737/1‑62 (MQ=255) cGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCt > 1:517920/1‑69 (MQ=255) aTCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAg < 1:2729506/46‑1 (MQ=255) aTCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc < 1:255075/71‑1 (MQ=255) aTCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc < 1:1264329/71‑1 (MQ=255) aTCATATTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAgg > 1:4392046/1‑54 (MQ=255) tCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATc > 1:4479026/1‑70 (MQ=255) cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAc > 1:792141/1‑63 (MQ=255) cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCAc > 1:2066141/1‑63 (MQ=255) cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg > 1:4427880/1‑66 (MQ=255) cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg > 1:2561815/1‑66 (MQ=255) cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg > 1:255050/1‑66 (MQ=255) cATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa > 1:4432024/1‑71 (MQ=255) atatTAAGGATAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCa > 1:2082587/1‑70 (MQ=255) aaGGTTAATACGATAGCTCTTTTCCAGATCggtgg > 1:2232639/1‑35 (MQ=255) aaGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCa > 1:3793510/1‑71 (MQ=255) gTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCg < 1:1164410/37‑1 (MQ=255) gTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTg < 1:2570382/57‑1 (MQ=255) ttAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAag < 1:529742/39‑1 (MQ=255) | AGCCATTCGGAGAGGATTTCCAGCAGGTTTTTCACCGCCGGACGACCATCCAGACCGATCATATTAAGGTTAATACGATAGCTCTTTTCCAGATCGGTGGTAGCGAAGAGGTGGTTCATCACCTGATCCATATCCA > W3110S.gb/3163578‑3163713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |