| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,204,990 | T→C | 28.1% | intergenic (‑78/‑129) | ygiP ← / → ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,204,990 | 0 | T | C | 28.1% | 145.7 / 74.2 | 96 | intergenic (‑78/‑129) | ygiP/ttdA | predicted DNA‑binding transcriptional regulator/L‑tartrate dehydratase, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base T (23/46); new base C (13/14); total (36/60) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
ATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAT > W3110S.gb/3204921‑3205059 | aTCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATattcatt > 1:3315003/1‑71 (MQ=255) aGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAtt < 1:1070100/71‑1 (MQ=255) gtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGGTCTGGCGCAATATTCATTCTTATTTTc > 1:1068606/1‑70 (MQ=255) gtgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTcg > 1:2762092/1‑71 (MQ=255) tgATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAt < 1:4105959/65‑1 (MQ=255) aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTcgcg < 1:2564894/70‑1 (MQ=255) aTAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGt < 1:2555/71‑1 (MQ=255) tAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGtt < 1:3910863/71‑1 (MQ=255) aGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTg < 1:2862226/70‑1 (MQ=255) tgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAt < 1:3826727/53‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAtt < 1:3984246/52‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAtt < 1:4184410/52‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTAtt < 1:158345/52‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAc > 1:419175/1‑71 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTAtttt < 1:250676/54‑1 (MQ=255) tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGa > 1:3895861/1‑62 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTa < 1:3391822/49‑1 (MQ=255) aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTa < 1:3735780/49‑1 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCtt < 1:77249/47‑1 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCtt < 1:2243074/47‑1 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGa < 1:3844138/67‑1 (MQ=255) tttCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTg > 1:437458/1‑71 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGt < 1:1856793/70‑1 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGt > 1:3865045/1‑70 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAAATGt > 1:4290499/1‑70 (MQ=255) tCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTAtt > 1:424171/1‑48 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:2575902/70‑1 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:3606802/70‑1 (MQ=255) cGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:671826/70‑1 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTa < 1:1266773/70‑1 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTa < 1:3538963/70‑1 (MQ=255) gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTa < 1:3533754/70‑1 (MQ=255) cAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGAtt < 1:832055/57‑1 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTa < 1:605852/42‑1 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTa < 1:3387070/42‑1 (MQ=255) aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:626083/1‑69 (MQ=255) aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:2553532/1‑68 (MQ=255) aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCa > 1:859696/1‑57 (MQ=255) aaaCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAAtt > 1:2499018/1‑70 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCtt < 1:2109398/39‑1 (MQ=255) aaCATTGCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTa > 1:4527543/1‑70 (MQ=255) aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAc > 1:3588475/1‑56 (MQ=255) aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACa < 1:4086989/71‑1 (MQ=255) cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGtt < 1:2540618/48‑1 (MQ=255) cATTGCCATTCCCTG‑‑CTGGCGCAATATTCATTCTTATTTTc > 1:1997603/1‑41 (MQ=37) ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGtt < 1:1056168/46‑1 (MQ=255) ttGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACatat < 1:1720521/71‑1 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAtt < 1:265862/70‑1 (MQ=255) gCCATTCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATAtt < 1:2683793/70‑1 (MQ=255) gCCATTCACTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:292364/1‑61 (MQ=255) ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACata < 1:27783/67‑1 (MQ=255) aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGaa > 1:4036025/1‑51 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGtt < 1:2986257/40‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa < 1:4565132/42‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGAtt < 1:1580816/44‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCa < 1:4319254/46‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCAc < 1:249551/47‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGa < 1:4336186/49‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:47405/55‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCACTCTTATTTTcg < 1:4367566/36‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGa < 1:611384/42‑1 (MQ=255) ttCCCTGCTCTGGCGCAATATTCACTCTTATTTTCGCCTTGa < 1:2319123/42‑1 (MQ=38) tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa > 1:3624110/1‑41 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGa > 1:3290725/1‑40 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCa > 1:1166128/1‑44 (MQ=255) cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGt > 1:2112168/1‑70 (MQ=255) cccTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGa > 1:1328321/1‑40 (MQ=255) cccTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTa < 1:3477407/58‑1 (MQ=255) cccTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACg > 1:1902536/1‑68 (MQ=255) cacTGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACgg > 1:1683480/3‑69 (MQ=255) ccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGtt < 1:2403342/37‑1 (MQ=255) tGCTCTGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:3009178/1‑52 (MQ=255) ctctGGCGCAATATTCACTCTTATTTTCGCGTTGATTCACGAACTg > 1:432383/1‑46 (MQ=255) tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaaa > 1:3769796/1‑70 (MQ=255) tatGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaaa > 1:333563/3‑70 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCa > 1:1101500/1‑37 (MQ=255) ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTAc < 1:63636/52‑1 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGaa > 1:3208475/1‑37 (MQ=255) cgcAATATTCATTCTTATTTTCGCGTTGATTCACGaa > 1:1020277/1‑37 (MQ=255) cAATATTCACTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:2082253/71‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCCCGAACTGtt < 1:2457692/38‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTg < 1:1395452/36‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGtt < 1:2642234/38‑1 (MQ=255) atatTCATTCTTATTTTCGCGTTGATTCACGAACTGt < 1:2467342/37‑1 (MQ=255) atatTCACTCTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:1908221/1‑40 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAAt < 1:195633/40‑1 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACa < 1:1634313/44‑1 (MQ=255) tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACg < 1:1294662/52‑1 (MQ=255) tatTCATTCTTATTTTCGAGTTGATTCACGAACTGTTAATTACATAtt < 1:1488928/48‑1 (MQ=255) tatTCATTCTTATTATCGCGTTGATTCACGAAATGTTAATTa < 1:4102041/42‑1 (MQ=38) attcattcTTATTTTCGCGTTGATTCACGAACTGTTaa > 1:2589631/1‑38 (MQ=255) attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATaa < 1:3682147/67‑1 (MQ=255) ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAATCca < 1:386206/71‑1 (MQ=255) ttcactcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACca > 1:808243/1‑71 (MQ=255) tcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCaaaaaa > 1:1435265/1‑60 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACcaca > 1:1081827/1‑71 (MQ=255) cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACcac < 1:1307258/70‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACcac < 1:208867/69‑1 (MQ=255) attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAt < 1:4269479/71‑1 (MQ=255) attcTTATTTTAGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAt < 1:1038915/71‑1 (MQ=255) | ATCCGCAGTTGTGATAAGCGCAGTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACAT > W3110S.gb/3204921‑3205059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |