| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,211,677 | C→T | 19.0% | intergenic (+169/‑26) | dnaG → / → rpoD | DNA primase/RNA polymerase, sigma 70 (sigma D) factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,211,677 | 0 | C | T | 19.0% | 195.3 / 37.1 | 84 | intergenic (+169/‑26) | dnaG/rpoD | DNA primase/RNA polymerase, sigma 70 (sigma D) factor |
| Reads supporting (aligned to +/- strand): ref base C (43/25); new base T (6/10); total (49/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.00e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01 | |||||||||||
AAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACC > W3110S.gb/3211612‑3211744 | aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGTGTCAATCGCCCAACACCAACCTCATGa > 1:4004438/1‑70 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACTTCATGa < 1:41/70‑1 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGa > 1:10340/1‑70 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGa > 1:2186508/1‑70 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGa > 1:103517/1‑70 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGa > 1:630460/1‑70 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGa > 1:404289/1‑70 (MQ=255)aaaCAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGa > 1:2645385/1‑70 (MQ=255) aacaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGaaa > 1:256292/1‑71 (MQ=255) acaacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAAt < 1:3537912/71‑1 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGAAAt > 1:1122829/1‑70 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAAt > 1:1111623/1‑70 (MQ=255) caacCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAAt > 1:4298343/1‑70 (MQ=255) acCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAg < 1:3434394/71‑1 (MQ=255) gTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATg < 1:1946055/61‑1 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATCGTGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg > 1:2372331/1‑71 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGtgtg < 1:623426/71‑1 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg > 1:160372/1‑71 (MQ=255) ttGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:2089523/71‑1 (MQ=255) tGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGaa > 1:3078345/1‑61 (MQ=255) ggATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGaaa > 1:3910011/1‑61 (MQ=255) ggATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGa > 1:3759871/1‑71 (MQ=255) cGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCt > 1:2118160/1‑70 (MQ=255) gTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTa < 1:3562721/71‑1 (MQ=255) ttAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTAt < 1:1154904/71‑1 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAgtg > 1:1777960/1‑54 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAgtg > 1:2683374/1‑54 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg > 1:3854571/1‑70 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg > 1:3125219/1‑70 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg > 1:1021749/1‑70 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg > 1:1071723/1‑70 (MQ=255) aaCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACAGTCTTATg > 1:1900165/1‑70 (MQ=255) aCGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg > 1:4102107/1‑69 (MQ=255) gCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGaa > 1:1277471/1‑43 (MQ=255) gAAGGACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGtgtg < 1:3301776/49‑1 (MQ=255) gAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaaa > 1:2500399/1‑71 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGtgtg < 1:3980064/48‑1 (MQ=255) aaGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:4525229/1‑71 (MQ=255) aGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATaa > 1:180514/1‑42 (MQ=255) ggACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAccc < 1:3628485/71‑1 (MQ=255) ggACATCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAccc < 1:2156505/71‑1 (MQ=255) cATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGAt < 1:3775847/46‑1 (MQ=255) tCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTTTGGATACCGTCt < 1:4533987/51‑1 (MQ=255) tCGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCt < 1:4539491/51‑1 (MQ=255) cGGGTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATAc > 1:3746052/1‑45 (MQ=255) cGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATgg > 1:2854469/1‑55 (MQ=255) gggTCAATCGCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTca > 1:3764437/1‑71 (MQ=255) gggTCAATCGCCCAACACCAACCTCATGAAATAAGtgtg < 1:2912551/39‑1 (MQ=255) gggTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGa > 1:3726855/1‑41 (MQ=255) gggTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTc < 1:2642218/70‑1 (MQ=255) gTCAATCGCCCAACACCAACCTTTTGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcaca < 1:4406729/71‑1 (MQ=255) gTCAATCGCCCAACACCAACCTCATGAAATAAGTGTgg > 1:2156874/1‑38 (MQ=255) gTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcaca < 1:3713253/71‑1 (MQ=255) tCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcaca < 1:2740273/70‑1 (MQ=255) aTCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:920734/1‑56 (MQ=255) aTCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:3703639/1‑56 (MQ=255) tCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATg < 1:4649443/46‑1 (MQ=255) gCCCAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:2138351/1‑71 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGTATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaa < 1:1004460/70‑1 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:446826/1‑71 (MQ=255) gCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGaaa > 1:1681160/1‑71 (MQ=255) cAACACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTcaca < 1:531864/61‑1 (MQ=255) aaCACCAACCTTATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGa < 1:1527347/65‑1 (MQ=255) aaCACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtc < 1:2648076/71‑1 (MQ=255) aaCACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtc < 1:622215/71‑1 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCtt < 1:2849961/36‑1 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCt < 1:1463787/35‑1 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCt < 1:229674/35‑1 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCTTa < 1:4606669/37‑1 (MQ=255) acacCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAAct < 1:2372523/68‑1 (MQ=255) cacCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAActtctt > 1:560630/1‑71 (MQ=255) acCAACCTCATGAAATAAGTGTGGATACCGTCTTATg > 1:4631932/1‑37 (MQ=255) acCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc > 1:1258450/1‑46 (MQ=255) acCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTg > 1:2041155/1‑71 (MQ=255) ccAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGt > 1:4010102/1‑71 (MQ=255) aaCCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCa > 1:1775415/1‑71 (MQ=255) aaCCTCATGAAATAAGTGTGGATACAGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCa > 1:828551/1‑71 (MQ=255) ccTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 1:2502407/39‑1 (MQ=255) ccTCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaa < 1:564980/39‑1 (MQ=255) ccTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCt < 1:2537905/55‑1 (MQ=255) ccTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCAcc > 1:3130797/1‑71 (MQ=255) tCATGAAATAAGTGTGGATACCGTCTTATGGAGCaaaa > 1:1445521/1‑38 (MQ=255) tCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAAc < 1:167637/39‑1 (MQ=255) tCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGa > 1:3011743/1‑55 (MQ=255) cATCAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTCaaa > 1:3086267/1‑56 (MQ=255) | AAACAACCGTTGGATTTCAGCGTTAACGGCTGAAGGACATCGGGTCAATCGCCCAACACCAACCTCATGAAATAAGTGTGGATACCGTCTTATGGAGCAAAACCCGCAGTCACAGCTGAAACTTCTTGTCACC > W3110S.gb/3211612‑3211744 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |