| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,272,348 | C→T | 40.5% | G89S (GGT→AGT) | garR ← | tartronate semialdehyde reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,272,348 | 0 | C | T | 40.5% | 49.6 / 84.5 | 74 | G89S (GGT→AGT) | garR | tartronate semialdehyde reductase |
| Reads supporting (aligned to +/- strand): ref base C (20/24); new base T (14/16); total (34/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01 | |||||||||||
AGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGG > W3110S.gb/3272284‑3272414 | aGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCtt < 1:3831791/71‑1 (MQ=255) gcgcTTCGCTGATTTCACGGCTTGCCAGCGGTGCGA‑A‑AACTCATATCGATCAATACCGTACCTGGCTTcgc < 1:3414188/71‑1 (MQ=255) cgcTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcg > 1:1563862/1‑71 (MQ=255) gcTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTcgc < 1:955133/71‑1 (MQ=255) gcTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTcgc < 1:1937233/71‑1 (MQ=255) gcTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcg > 1:3009037/1‑70 (MQ=255) cGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCgcgc > 1:2799022/1‑69 (MQ=255) cGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCt > 1:4235784/1‑71 (MQ=255) cTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCt < 1:1855581/61‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGc > 1:3623648/1‑57 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTcgcg < 1:2025281/63‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:4286505/71‑1 (MQ=255) aTTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaat < 1:3846851/71‑1 (MQ=255) tttCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaata < 1:2084455/71‑1 (MQ=255) aCGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGc < 1:24885/71‑1 (MQ=255) cGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGcc < 1:942345/71‑1 (MQ=255) gCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCAt > 1:4464487/1‑71 (MQ=255) tGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCAtt < 1:4191695/69‑1 (MQ=255) tGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCAtt < 1:2853476/69‑1 (MQ=255) tGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCAtt < 1:4403066/69‑1 (MQ=255) gCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATtct < 1:303/70‑1 (MQ=255) ccAGCGGTGCGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATtctc < 1:2340183/70‑1 (MQ=255) ggTGCGATAGAACTCATATCGATCAATACCGTACCTGGCtt > 1:1428847/1‑41 (MQ=255) ggTGCGATAGAACTCATATCGATCAATACCGTACCTGGCtt > 1:3534902/1‑41 (MQ=255) cGATAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGcc < 1:980483/71‑1 (MQ=255) cGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCa < 1:4228658/56‑1 (MQ=255) cGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGcc < 1:1365391/71‑1 (MQ=255) aTAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGc < 1:542907/52‑1 (MQ=255) aTACAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCAtt > 1:4455537/1‑56 (MQ=255) tAGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAcc < 1:3164881/71‑1 (MQ=255) tAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaata > 1:590898/1‑47 (MQ=255) tAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaaaa > 1:2971525/1‑45 (MQ=255) tAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCaa > 1:3871551/1‑45 (MQ=255) aGAATTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACct > 1:425994/1‑71 (MQ=255) aGAACTCATATCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACct > 1:213590/1‑71 (MQ=255) tCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCTATAATGCCATTCTCACCCAGCGCCAc < 1:2629840/64‑1 (MQ=255) atatCGATCAATACCGTACTTGGCTTCGCGCCTTCaataa > 1:2307593/1‑40 (MQ=255) atatCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAgcg > 1:3701452/1‑58 (MQ=255) atatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:4469871/70‑1 (MQ=255) tatCGATCAATACCGTACTTGGCTTCGCGCCTTCa < 1:766385/35‑1 (MQ=255) tatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:3359703/69‑1 (MQ=255) tatCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTca < 1:2733210/69‑1 (MQ=255) atCGATCAATACCGTACCTGGCTTCGCGCCTTCaa > 1:531623/1‑35 (MQ=255) atCGATCAATACCGTACCTGGCTTCGCGCCTTCaa < 1:677157/35‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTcac > 1:2763330/1‑68 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:1917008/1‑70 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:4073580/1‑70 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:1231451/70‑1 (MQ=255) tCGATCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:220741/70‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCAt > 1:180334/1‑43 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:3926279/70‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:327459/1‑70 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:1260960/1‑70 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:4135641/70‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:443558/70‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:1036198/71‑1 (MQ=255) tCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATg < 1:2546328/71‑1 (MQ=255) cGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt > 1:3867814/1‑69 (MQ=255) gATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCttt < 1:2201046/63‑1 (MQ=255) aTCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:856454/67‑1 (MQ=255) aTCAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACAt < 1:818781/67‑1 (MQ=255) aTCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAgg < 1:1801794/71‑1 (MQ=255) cAATACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCAACCAGCGCAACCTCTTTCACATGAggg > 1:950629/1‑70 (MQ=255) cAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCa > 1:4607606/1‑53 (MQ=255) aaaTACCGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAggg > 1:3011130/2‑70 (MQ=255) tACCGTACTTGGCTTCGCGCCTTTAATAATGCCATTCTCACCCAGCGCCAc > 1:1572644/1‑51 (MQ=255) ccGTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAc > 1:2120231/1‑49 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCa > 1:2809553/1‑48 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCAc > 1:2931494/1‑49 (MQ=255) ccGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGttt < 1:4029368/71‑1 (MQ=255) gTACTTGGCTTCGCGCCTTCAATAATGCCATTCTCa > 1:1246207/1‑36 (MQ=255) gTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTgg < 1:4083854/71‑1 (MQ=255) gTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTg > 1:4021327/1‑70 (MQ=255) tACTTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTgg > 1:847428/1‑70 (MQ=255) | AGCGCTTCGCTGATTTCACGGCTTGCCAGCGGTGCGATAGAACTCATATCGATCAATACCGTACCTGGCTTCGCGCCTTCAATAATGCCATTCTCACCCAGCGCCACCTCTTTCACATGAGGGGAGTTTGG > W3110S.gb/3272284‑3272414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |