| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 57,363 | T→C | 9.1% | intergenic (‑254/‑1) | imp ← / → djlA | exported protein required for envelope biosynthesis and integrity/DnaJ‑like protein, membrane anchored |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 57,363 | 0 | T | C | 9.1% | 143.0 / 6.5 | 55 | intergenic (‑254/‑1) | imp/djlA | exported protein required for envelope biosynthesis and integrity/DnaJ‑like protein, membrane anchored |
| Reads supporting (aligned to +/- strand): ref base T (16/34); new base C (2/3); total (18/37) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.37e-01 | |||||||||||
AAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGT > W3110S.gb/57297‑57431 | aaaCGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGc < 1:3438750/71‑1 (MQ=255) aaaGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTgg > 1:1309475/1‑71 (MQ=255) ccTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaaa < 1:2780163/71‑1 (MQ=255) ttATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt > 1:1726293/1‑70 (MQ=255) ttATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt > 1:2910655/1‑70 (MQ=255) ttATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAAt > 1:1338035/1‑70 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATc < 1:1571185/70‑1 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa > 1:2128308/1‑71 (MQ=255) tataTTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCa > 1:2183788/1‑71 (MQ=255) ttGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGt < 1:2340471/52‑1 (MQ=255) ttGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTgg < 1:1752307/71‑1 (MQ=255) ttGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTgg < 1:668312/71‑1 (MQ=255) gtgGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCAt > 1:686473/1‑66 (MQ=255) tgGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcg < 1:594033/70‑1 (MQ=255) ggAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaa < 1:73921/57‑1 (MQ=255) ggAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaa < 1:3563137/57‑1 (MQ=255) ggAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaa < 1:122036/57‑1 (MQ=255) ggAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaa < 1:1010578/57‑1 (MQ=255) gagaTAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggc < 1:2051480/71‑1 (MQ=255) aGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTa > 1:362249/1‑42 (MQ=255) aGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTa > 1:3245462/1‑42 (MQ=255) aGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTgg > 1:3552853/1‑46 (MQ=255) gCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggccgtggc < 1:184431/71‑1 (MQ=255) tGATATCCGTGTGTCGATTTGGGGAATATATGCAg < 1:2670324/35‑1 (MQ=255) tGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTa > 1:804546/1‑71 (MQ=255) atatCCGTGTGTCGATTTGGGGAATATATGCAGTa > 1:2414714/1‑35 (MQ=255) atatCCGTGTGTCGATTTGGGGAATATATGCAGTa > 1:1319023/1‑35 (MQ=255) atatCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTAc > 1:420011/1‑70 (MQ=255) tatCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtg < 1:160975/56‑1 (MQ=255) tatCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtg < 1:831107/56‑1 (MQ=255) tatCCGTGTGTCGATTTGGGGAATACATGCAGTATTGGGGAAAAAt < 1:4150928/46‑1 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGaaaa < 1:4569781/42‑1 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCt < 1:2879381/64‑1 (MQ=255) tCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACt < 1:4398908/68‑1 (MQ=255) tgtgtCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggc > 1:1076771/1‑71 (MQ=255) tgtCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcg < 1:1123518/70‑1 (MQ=255) tgtCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcg < 1:262185/70‑1 (MQ=255) gtCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGa < 1:409548/63‑1 (MQ=255) tCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTgg < 1:2988195/42‑1 (MQ=255) tCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACt < 1:457636/60‑1 (MQ=255) tCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGa < 1:2332731/62‑1 (MQ=255) tCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACt < 1:2669417/60‑1 (MQ=255) tCGATTTGGGGAATACATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcg > 1:154341/1‑71 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcggc < 1:437564/71‑1 (MQ=255) gATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGggcggcgg > 1:2833206/1‑70 (MQ=255) aTTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGcgtggc < 1:3025540/45‑1 (MQ=255) tttGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCtt < 1:1754725/71‑1 (MQ=255) tttGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCtt < 1:978338/71‑1 (MQ=255) gggAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTg < 1:1462473/70‑1 (MQ=255) gggAATACATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTg < 1:3812498/70‑1 (MQ=255) ggAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTggg < 1:2584217/71‑1 (MQ=255) taaTATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCt < 1:1926450/45‑1 (MQ=255) gAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACt < 1:2503101/50‑1 (MQ=255) aaTATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCt < 1:3413714/45‑1 (MQ=255) aaTATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGG‑GGCTTTTGGGGCg > 1:1934138/1‑71 (MQ=255) tatatGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGt < 1:3944590/71‑1 (MQ=255) | AAACGGTAAAAGCACCTTTATATTGTGGGAGATAGCCCTGATATCCGTGTGTCGATTTGGGGAATATATGCAGTATTGGGGAAAAATCATTGGCGTGGCCGTGGCCTTACTGATGGGCGGCGGCTTTTGGGGCGT > W3110S.gb/57297‑57431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |