| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,328,359 | T→C | 20.8% | N71S (AAC→AGC) | greA ← | transcription elongation factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,328,359 | 0 | T | C | 20.8% | 103.0 / 25.7 | 53 | N71S (AAC→AGC) | greA | transcription elongation factor |
| Reads supporting (aligned to +/- strand): ref base T (23/19); new base C (6/5); total (29/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-01 | |||||||||||
CGTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACGAGCTGCG > W3110S.gb/3328292‑3328424 | cGTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc < 1:3299325/70‑1 (MQ=255)cGTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc < 1:2749629/70‑1 (MQ=255) gTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGa > 1:1734321/1‑71 (MQ=255) aCGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAg > 1:664656/1‑71 (MQ=255) gTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCtt < 1:1517250/71‑1 (MQ=255) aGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGa > 1:4578708/1‑63 (MQ=255) aGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGa > 1:3199649/1‑63 (MQ=255) gCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGc > 1:4297463/1‑71 (MQ=255) ccAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTc < 1:1669837/71‑1 (MQ=255) aaaaaTAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGa > 1:1579385/1‑71 (MQ=255) aaaaTAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGAt < 1:2668055/71‑1 (MQ=255) aaTAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCTCGTTCGACAGCTTGGCTTCGATg > 1:4001338/1‑70 (MQ=255) aTAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTc < 1:4331016/71‑1 (MQ=255) cgcgCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTc > 1:3539101/1‑47 (MQ=255) cgcgCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTa < 1:2856202/71‑1 (MQ=255) gcgcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTaa < 1:3216632/71‑1 (MQ=255) gcgcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTaa < 1:3393572/71‑1 (MQ=255) cgcCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTa < 1:2051134/69‑1 (MQ=255) cATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCtt < 1:1093155/49‑1 (MQ=255) cATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCt > 1:2825553/1‑62 (MQ=255) aTTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGCTCGa < 1:1253069/42‑1 (MQ=255) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTgg > 1:4311312/1‑49 (MQ=255) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTc > 1:4338137/1‑53 (MQ=255) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTc > 1:3273744/1‑53 (MQ=255) ttgttgGGCATTTTGGTGACATCAATCACCTGCGCGCTCGACAGCtt < 1:2264425/47‑1 (MQ=255) tgttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCt > 1:3256930/1‑71 (MQ=255) gttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGc > 1:2440248/1‑43 (MQ=255) gttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTgg > 1:3927719/1‑47 (MQ=255) gttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTgg > 1:2289339/1‑47 (MQ=255) gttgGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATg > 1:3630914/1‑55 (MQ=255) gggCATTTTGGTGACATCAATCACCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCg < 1:2693327/70‑1 (MQ=255) ttttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTc < 1:2669606/43‑1 (MQ=255) ttttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGaaa < 1:997233/71‑1 (MQ=255) ttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGa > 1:4009064/1‑43 (MQ=255) ttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAAcc > 1:1636236/1‑71 (MQ=255) ttGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAAc < 1:2642275/70‑1 (MQ=255) gTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAAACctg < 1:2799834/71‑1 (MQ=255) cATCAATCACCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGt > 1:2667231/1‑71 (MQ=255) atcaatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGtt > 1:4428749/1‑71 (MQ=255) atcaatcaCCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGGCTTCGCAGAAACCCTGCTGtt > 1:1906662/1‑71 (MQ=255) atcaatcaCCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGtt > 1:4174132/1‑71 (MQ=255) atcaatcaCCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGtt > 1:4273094/1‑71 (MQ=255) atcaatcaCCTGCGCGCTCGACAGCTTGGCTGCGATGTCttt < 1:4026544/42‑1 (MQ=37) caatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCa > 1:702435/1‑71 (MQ=255) aatcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATAc > 1:773308/1‑44 (MQ=255) aatcaCCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCAc > 1:1675646/1‑71 (MQ=255) atcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCttt < 1:3514150/38‑1 (MQ=255) atcaCCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACg < 1:2903715/71‑1 (MQ=255) tcaCCTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACg < 1:2365722/70‑1 (MQ=255) cTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACGAGCTg < 1:2990003/71‑1 (MQ=255) cTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACGAGCTg < 1:1440013/71‑1 (MQ=255) cTGCGCGCTCGACAGCTTGGCTTCGATGTCTTTAATa > 1:1260680/1‑37 (MQ=255) gcgcGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACGAGCTGCg > 1:2425807/1‑71 (MQ=255) | CGTTACGGTAGCACCAAAAATAACGCGCCCATTGTTGGGCATTTTGGTGACATCAATCACCTGCGCGTTCGACAGCTTGGCTTCGATGTCTTTAATACGGCCTTCGCAGAAACCCTGCTGTTCACGAGCTGCG > W3110S.gb/3328292‑3328424 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |