| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,332,723 | A→G | 22.2% | A84A (GCT→GCC) | rpmA ← | 50S ribosomal subunit protein L27 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,332,723 | 0 | A | G | 22.2% | 86.0 / 22.7 | 45 | A84A (GCT→GCC) | rpmA | 50S ribosomal subunit protein L27 |
| Reads supporting (aligned to +/- strand): ref base A (17/18); new base G (7/3); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
GAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAG > W3110S.gb/3332656‑3332793 | gAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCt > 1:604647/1‑71 (MQ=255) aTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc < 1:3727992/71‑1 (MQ=255) tAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCGGCTTCGAt > 1:4296272/1‑71 (MQ=255) tAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCGGCTTCGAt > 1:4301697/1‑71 (MQ=255) gCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGAt < 1:405992/71‑1 (MQ=255) ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGc < 1:1831564/56‑1 (MQ=255) ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaaa > 1:1676833/1‑71 (MQ=255) cGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAt > 1:1485348/1‑71 (MQ=255) gCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAtt < 1:1622716/71‑1 (MQ=255) cAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGAt > 1:627777/1‑59 (MQ=255) cGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCGGCTTCGa > 1:3114532/1‑55 (MQ=255) cGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCGGCTTCGa > 1:3551592/1‑55 (MQ=255) ttGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc < 1:7663/49‑1 (MQ=255) ttGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaaa < 1:241965/61‑1 (MQ=255) ttGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGttt < 1:1678597/71‑1 (MQ=255) gCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACgg > 1:2871815/1‑66 (MQ=255) cGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGttttt < 1:1211454/70‑1 (MQ=255) cGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTc < 1:1412522/71‑1 (MQ=255) gggCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc < 1:1565689/44‑1 (MQ=255) cTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGATTCGATGCTGa > 1:3287550/1‑49 (MQ=255) tttCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTa > 1:4394239/1‑56 (MQ=255) ttCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc < 1:2818303/39‑1 (MQ=255) ttCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc < 1:619328/39‑1 (MQ=255) tCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt > 1:1349629/1‑71 (MQ=255) cATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGa > 1:3464036/1‑39 (MQ=255) cATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt > 1:3043283/1‑70 (MQ=255) ccGTTACCGGGACGCGAAAAACTTATTCAGCTTCGa > 1:1535593/1‑36 (MQ=255) ccGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACg > 1:2158847/1‑52 (MQ=255) ccGGGACGCGAAAAACTTATTCGGCTTCGATGCTGATaa < 1:896515/39‑1 (MQ=255) ggACGCGAAAAACTTATTCGGCTTCGATGCTGATAAATTTACg < 1:1376232/43‑1 (MQ=255) aaaaaCTTATTCGGCTTCGATGCTGATAAATTTACGGttttt > 1:292751/1‑42 (MQ=255) aaaaaCTTATTCGGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGa < 1:4157505/59‑1 (MQ=255) aaaaaCTTATTCAGCTTCGATGCTGATAAATTTACGGttt < 1:4217132/40‑1 (MQ=255) aaaaaCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTc < 1:3894809/43‑1 (MQ=255) aaaaaCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCg < 1:3403187/58‑1 (MQ=255) aaaaaCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTa < 1:1831396/70‑1 (MQ=255) aaaCTTATTCAGCTTCGATGCTGATAAATTTACgg > 1:2197119/1‑35 (MQ=255) aaaCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc > 1:4053679/1‑45 (MQ=255) aaaCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc > 1:3420049/1‑45 (MQ=255) aaaCTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCa > 1:4413652/1‑63 (MQ=255) aCTTATTCGGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTc > 1:1265792/1‑71 (MQ=255) aCTTATTCGGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTc > 1:464354/1‑71 (MQ=255) ttCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTcttctt < 1:3534122/71‑1 (MQ=255) ttCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCtt < 1:58421/71‑1 (MQ=255) cAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTAc > 1:2773041/1‑60 (MQ=255) aGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCtt > 1:315919/1‑68 (MQ=255) aGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAg > 1:3790464/1‑71 (MQ=255) | GAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAG > W3110S.gb/3332656‑3332793 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |