| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | W3110S.gb | 3,348,085 | G→A | 38.6% | E89K (GAA→AAA) | npr → | phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 3,348,085 | 0 | G | A | 38.6% | 35.8 / 44.0 | 44 | E89K (GAA→AAA) | npr | phosphohistidinoprotein‑hexose phosphotransferase component of N‑regulated PTS system (Npr) |
| Reads supporting (aligned to +/- strand): ref base G (12/15); new base A (5/12); total (17/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAAGCGACCGGTCCACAGGAAGAGGAAGCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTAC > W3110S.gb/3348016‑3348155 | gaagCGACCGGTCCACAGGAAGAGGAAGCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTtgatg < 1:3177806/70‑1 (MQ=255) cGACCGGTCCACAGGAAGAGGAAGCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGAt < 1:3021145/71‑1 (MQ=255) ccGGTCCACAGGAAGAGGAAGCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTaa < 1:3681246/71‑1 (MQ=255) cacaGGAAGAGGAAGCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTtca > 1:1401496/1‑71 (MQ=255) ggaagaggaagCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCAc < 1:1690297/71‑1 (MQ=255) agaggaagCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTtcatca > 1:4044519/1‑67 (MQ=255) agaggaagCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACttt < 1:530683/71‑1 (MQ=255) agaggaagCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACttt < 1:392791/71‑1 (MQ=255) agaggaagCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACttt < 1:387500/71‑1 (MQ=255) gaggaagCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTg < 1:481201/71‑1 (MQ=255) agCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATa > 1:2580726/1‑71 (MQ=255) gCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATAc < 1:484355/71‑1 (MQ=255) tGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGaa < 1:167620/41‑1 (MQ=255) tGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGa < 1:4136553/43‑1 (MQ=255) tGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTaa < 1:754313/47‑1 (MQ=255) tGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACTTTGa < 1:1194265/62‑1 (MQ=255) tGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACTTTGACATACAACt > 1:4383592/1‑71 (MQ=255) ggCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTaa > 1:785506/1‑46 (MQ=255) gccgTTATCGCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACttt < 1:314910/55‑1 (MQ=255) cgTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCa < 1:2475534/71‑1 (MQ=255) ttATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTa < 1:652008/37‑1 (MQ=255) ttATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATAc < 1:1110173/58‑1 (MQ=255) tATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCAc > 1:2506021/1‑47 (MQ=255) tATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCAc > 1:562776/1‑47 (MQ=255) tATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCaaa > 1:1388036/1‑70 (MQ=255) aTCTCCCTCTTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACt > 1:317115/1‑71 (MQ=255) aTCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTtcatc > 1:51794/1‑44 (MQ=255) aTCGCCCTCTTTAATTCTGGGTTTGATGAAGATTAATCTTCATCACTTTGACATACAACt < 1:4456388/60‑1 (MQ=255) ctctTTAATTCTGGTTTTGATAAAGATTAATCTTCATCACTTTGACa < 1:2913938/47‑1 (MQ=255) tctTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCa < 1:1888672/60‑1 (MQ=255) ctTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCaaa > 1:1227151/1‑61 (MQ=255) tttAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCaaa > 1:1367787/1‑60 (MQ=255) tttAATTCTGGTTTTGATAAAGATTAATCTTCATCACttt < 1:1490812/40‑1 (MQ=255) tttAATTCTGGTTTTGATAAAGATTAATCTTCATCACttt < 1:3372185/40‑1 (MQ=255) ttAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACt > 1:4428723/1‑61 (MQ=255) ttAATTCTGGTTTTGATAAAGATTAATCTtcatca > 1:1907901/1‑35 (MQ=255) tAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACaa > 1:1562613/1‑47 (MQ=255) tAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACaa > 1:3567184/1‑71 (MQ=255) ttCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATaa < 1:717983/42‑2 (MQ=255) ttCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATaa < 1:2042774/71‑1 (MQ=255) cTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATAc < 1:4213071/40‑1 (MQ=255) cTGGTTTTGATAAAGATTAATCTTCATCACTTTGa > 1:3046139/1‑35 (MQ=255) ggTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACaa < 1:3510120/64‑1 (MQ=255) gTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGaaa < 1:2137730/70‑1 (MQ=255) gTTTTGATAAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGaa < 1:52463/69‑1 (MQ=255) gatgaAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCAc < 1:3913297/70‑1 (MQ=255) tgaAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTa < 1:481409/71‑1 (MQ=255) tgaAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTa < 1:2837537/71‑1 (MQ=255) gaAGATTAATCTTCATCACTTTGACATACAACTCCCTTCaaa > 1:3163823/1‑42 (MQ=255) gaAGATTAATCTTCATCACTTTGACATACAACTCCCTTCaaa > 1:1728611/1‑42 (MQ=255) gaAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTAc > 1:3668509/1‑71 (MQ=255) | GAAGCGACCGGTCCACAGGAAGAGGAAGCACTGGCCGCCGTTATCGCCCTCTTTAATTCTGGTTTTGATGAAGATTAATCTTCATCACTTTGACATACAACTCCCTTCAAACTCCCCCCGACAATAAGAAAATCACGTAC > W3110S.gb/3348016‑3348155 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |