Predicted mutation
evidence seq id position mutation freq annotation gene description
RA W3110S.gb 3,359,906 T→C 28.8% M285T (ATG→ACG)  gltD → glutamate synthase, 4Fe‑4S protein, small subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb3,359,9060TC28.8% 96.6 / 49.0 66M285T (ATG→ACG) gltDglutamate synthase, 4Fe‑4S protein, small subunit
Reads supporting (aligned to +/- strand):  ref base T (20/27);  new base C (8/11);  total (28/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTA  >  W3110S.gb/3359836‑3359965
                                                                      |                                                           
ccGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCAt                                                             >  1:1038056/1‑71 (MQ=255)
 cGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATg                                                            >  1:2271936/1‑71 (MQ=255)
  gTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATg                                                            <  1:2614029/70‑1 (MQ=255)
       tCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGaa                                                         <  1:4247388/68‑1 (MQ=255)
        cATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGc                                                      >  1:1176295/1‑70 (MQ=255)
             ccAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAAcgc                                                >  1:256508/1‑71 (MQ=255)
              cAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAAcgcg                                               <  1:1928044/71‑1 (MQ=255)
                acacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCgtg                                             <  1:4404987/71‑1 (MQ=255)
                acacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCgtg                                             <  1:943930/71‑1 (MQ=255)
                acacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCgtg                                             <  1:2375154/71‑1 (MQ=255)
                acacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCgtg                                             <  1:2008556/71‑1 (MQ=255)
                 cacCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAg                                                        >  1:4210147/1‑59 (MQ=255)
                  acaaaaCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCaaa                                                   >  1:2990798/4‑63 (MQ=255)
                  acCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCgtggt                                           >  1:4214245/1‑71 (MQ=255)
                  acCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCgtggt                                           >  1:2305725/1‑71 (MQ=255)
                  acCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTAGTCAGCACGGAAGGCAAACGCgtggt                                           >  1:668858/1‑71 (MQ=255)
                   ccAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCgtggtg                                          >  1:4014540/1‑71 (MQ=255)
                     aaaCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGtggtgg                                         <  1:317458/70‑1 (MQ=255)
                       aCAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTcc                                      <  1:2176280/71‑1 (MQ=255)
                        cAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCt                                     <  1:3545667/71‑1 (MQ=255)
                         aGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCtt                                    <  1:4006399/71‑1 (MQ=255)
                          gTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAgg                                                       <  1:3957459/51‑1 (MQ=255)
                            tAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGa                                                          >  1:146508/1‑46 (MQ=255)
                            tAATGGGCTTTGGTGAAACCCGCGAAGAACCGTTCGTCAGCACGGaa                                                         >  1:1268793/1‑47 (MQ=255)
                             aaTGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                 <  1:3774926/70‑1 (MQ=255)
                             aaTGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                 <  1:3717020/70‑1 (MQ=255)
                               tGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCa                                                     <  1:1657424/48‑1 (MQ=255)
                               tGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCa                                                     <  1:372532/48‑1 (MQ=255)
                               tGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCt                                     >  1:71409/1‑64 (MQ=255)
                                 ggCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCa                                                     <  1:4549553/46‑1 (MQ=255)
                                  gCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                           >  1:999905/1‑71 (MQ=255)
                                  gCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                           <  1:4529076/71‑1 (MQ=255)
                                    tttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGt                              <  1:3191985/66‑1 (MQ=255)
                                    tttGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGa                         >  1:3844414/1‑71 (MQ=255)
                                      tGGTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGa                         <  1:1709769/69‑1 (MQ=255)
                                       ggTGAAACCCGCGACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGc                           <  1:4164237/66‑1 (MQ=255)
                                           aaaCCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                   >  1:970443/1‑70 (MQ=255)
                                               ccGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGa                 >  1:3680530/1‑68 (MQ=255)
                                               ccGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGa                 >  1:1553173/1‑68 (MQ=255)
                                               ccGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATg               <  1:2836347/70‑1 (MQ=255)
                                                cgcgACGAACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGa             >  1:196306/1‑71 (MQ=255)
                                                 gcgACGAACCGTTCGTCATCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc            >  1:393283/1‑71 (MQ=255)
                                                 gcgACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc            >  1:3832757/1‑71 (MQ=255)
                                                  cgacgaACCGTTCGTCAGCACGGAAGGCAAACGCgtggt                                           >  1:3853501/1‑39 (MQ=255)
                                                  cgacgaACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGAc            >  1:2194718/1‑70 (MQ=255)
                                                    acgaACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgc         <  1:3787093/71‑1 (MQ=255)
                                                      gaACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                 <  1:1917874/45‑1 (MQ=255)
                                                      gaACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                 <  1:82898/45‑1 (MQ=255)
                                                      gaACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                   <  1:4021197/59‑1 (MQ=255)
                                                      gaACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                   <  1:2640517/59‑1 (MQ=255)
                                                      gaACCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGc                   <  1:2014167/59‑1 (MQ=255)
                                                        aCCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgcgtgc     >  1:4482060/1‑71 (MQ=255)
                                                        aCCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgcgtgc     >  1:3239664/1‑71 (MQ=255)
                                                        aCCGTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGa                 >  1:606664/1‑59 (MQ=255)
                                                         ccGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGa                         >  1:2768365/1‑50 (MQ=255)
                                                         ccGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgcgtgcg    >  1:1579626/1‑71 (MQ=255)
                                                           gTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACTCTGCGATGGACTGCGTGCGTa  <  1:2907387/71‑1 (MQ=255)
                                                           gTTCGTCAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTa  <  1:737639/71‑1 (MQ=255)
                                                              cGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGac                        <  1:2278435/46‑1 (MQ=255)
                                                              cGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACt                     >  1:1084726/1‑49 (MQ=255)
                                                               gTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgcg        <  1:4087412/61‑1 (MQ=255)
                                                               gTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtgcg        <  1:284451/61‑1 (MQ=255)
                                                                tCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGc                                 <  1:1669338/35‑1 (MQ=255)
                                                                tCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtg          <  1:665519/58‑1 (MQ=255)
                                                                 cAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACtg          <  1:2864597/57‑1 (MQ=255)
                                                                 cAGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATg               <  1:3396196/52‑1 (MQ=255)
                                                                  aGCACGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTgcgtgcgt   <  1:3801288/63‑1 (MQ=255)
                                                                      |                                                           
CCGTTCCTCATCGCCAACACCAAACAGTTAATGGGCTTTGGTGAAACCCGCGACGAACCGTTCGTCAGCATGGAAGGCAAACGCGTGGTGGTCCTTGGCGGTGGCGACACTGCGATGGACTGCGTGCGTA  >  W3110S.gb/3359836‑3359965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: